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We narrowed to 354 results for: HRE

Showing: 21 - 40 of 354 results

  1. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ..., Width, and Area Within FSC and SSC, there are three parameters that are being measured: height (H), ...research fields, for any experiment, your first three gates will likely be: the FSC/SSC gate to identify...to choose either one to use. With our first three gates out of the way, it’s time to move on to my... scattering, you also discovered that there are three parameters — H, W, and A — describing the intensity...

  2. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...their ability to infect non-dividing cells. Thus three different generations of lentiviral vectors have...1st generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector genome...RCL generation because this vector requires only three HIV-1 genes (gag, pol, and rev) for production. ...viral particles to determine their concentration. Three methods are broadly used for virus titration: the...

  3. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...The recent conversations with three lab heads have revealed that a combination of hard work, determination... the PI Interview Series investigates how these three PIs reward their team and manage work-life balance...family reunions in various wildernesses and enjoys “three generations walk[ing] together in the Arboretum ...

  4. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...at least one semester (the majority spend two to three) working in your chosen field. This cooperative ...training process was lined out to span the first three months of my six month employment. In the beginning...that it is possible to work the remainder of the three hours (outside of the core hours) at a time that...

  5. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...about the popular technique incorrectly known as “three-parent babies,” where fused genetic material from... assistive reproductive technique was feasible, three separate things happened nearly simultaneously: ... agree upon a moratorium until another meeting, three branches – a private funder, government regulators...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...mouse and ferret models. Furthermore, they created three affinity variants – A184S (increased affinity for...A184V (medium affinity for glutamate), that came in three different colors – blue (SF-Azurite-iGluSnFR), green...affinity, and ∆F/F0. Introduce the two variants in three different membrane-targeting flavors – PDGFR, GPI...

  7. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...figure 1). While the codon used is often one of the three stop codons ­– most commonly the UAG codon – in ...need to be individually optimized for each of the three factors: the ncAA, the tRNA, and the tRNA synthetases...are the optimization parameters the same for all three components, meaning that the final protocol may ...

  8. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...creating three other photoswitchable kinases: psMEK2, psRaf1, and psCDK5. MEK2 is a serine/threonine kinase...but Raf1 and CDK5 have less similarity. For these three kinases, photoswitchable Dronpa domains were inserted...

  9. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...questions can it help answer? Ramanathan et al. outline three applications of RaPID: Discovery. To discover novel...Ramanathan et al. designed an RNA bait containing three well conserved but not well characterized RNA motifs...iron levels seen in HHCS patients. These are just three examples of how RaPID can be used to study RNA-protein...

  10. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...alter excitability by lowering or raising the threshold for signaling. The two main classes of receptors... The DREADD hM3D(Gq), for example, lowers the threshold for neuronal signaling and causes neuronal burst...DREADDs, such as the KORD receptor, increase this threshold and silence neurons. DREADD-activated GPCR signaling...

  11. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ... Toolbox and commercially available reagents in three major steps - all without the need to find a llama...antigen. The Seeger lab can directly provide the three sybody libraries in the form of mRNA for academic...SB_convex. These scaffolds contain serines and threonines at positions that can be randomized for creating...

  12. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...homology arms. MMEJ: An Introduction There are three primary methods for repairing DNA after a double-stranded...insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary for knock-in...-locus-specific gRNA cleaves genomic DNA. These three double stranded breaks are repaired by two rounds...

  13. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ... We can think of the human genome as a map with three coordinates: chromosome, start, and stop. For instance...One hypothesis: the enhancers! This leads us to a three-step approach for designing an experiment to identify...are wrong. In reality, cellular DNA is a complex three-dimensional globule of chromatin. Chromatin is a...

  14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ... DNA-targeting module together with the crRNA.  Three transposase proteins (TnsA, TnsB, TnsC). The donor... but instead employ a Cas12k protein instead of three Cas proteins (Fig. 1A, bottom), and require only...the Sternberg group’s initial report of VchINT, three separate plasmids and multiple promoters were used...

  16. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...viral vectors have changed the scope and power of three commonly used behavioral assays - locomotion, anxiety...associated) compared to an animal running to escape a threat. Locomotion can thus be used as a readout for associated...associated with psychiatric diseases such as schizophrenia or bipolar disorder. In both diseases, animals...

  17. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...different codons that encode 20 amino acids and three stop codons, meaning that the same amino acid can...One part of the tRNA contains the anticodon,  a three nucleotide sequence complementary to the mRNA sequence...the 64 codons that encodes the 20 amino acids and three stop codons. (B) Example of codon usage among different...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...content_id":null,"href":"https://www.addgene.org/collections/mammalianrnai/","href_with_scheme":"https://www.addgene.org... plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence Choosing...

  19. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...have been a staple in molecular biology for over three decades. Widely used across various research applications...Specifically for our knowledge here, the Pol gene encodes three separate enzymes that are proteolytically processed... entry into the host cell.  Transfecting these three plasmids into cells enables the production of lentiviral...

  20. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...proteins (5). Optimization of this system defined three components required for translation (Figure 1): ...translation of the pSer-incorporated polypeptide. These three components were shown to effectively incorporate...that wasn’t originally a serine (e.g. mutating a threonine residue to a phosphoserine) to control for effects...
Showing: 21 - 40 of 354 results