...Physical titer (particle count, rAdV genomes/mL) can be measured by: Optical density: particle concentration...260nm corresponds to 1.1 x 1012 particles/mL. To calculate the particle number: OD260 reading x dilution...dilution factor x 1.1 x 1012 particles = number of particles per mL of sample. The purity of the sample can...vectors The adenovirus virion is a non-enveloped particle of ~100 nm, consisting of a capsid surrounding...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...Expanding the optogenetics toolbox with CRY2clust Article contributed by Brook Pyhtila  Listen to the CRY2clust...novel opsin that enables single-cell optogenetics Article contributed by Meghan Rego  Listen to the soCoChR...29184208.        Synthetic decaffeination operon Article contributed by Angela Kaczmarczyk  Listen to the... for live-cell imaging of biochemical activity Article contributed by Angela Holmes Jin Zhang’s lab is...Listen to the FLIM-FRET acceptor podcast segment Article contributed by Christina Mork The Murakoshi lab...

.... Physical titers measure the amount of viral particles in a sample and are usually based on the presence...infectious titers, measure how many of the viral particles produced can actually infect cells. These assays...whether or not it is incorporated into a viral particle. Consequently, titers based off of p24 quantification...they can include free p24 and defective viral particles. Direct measurement of lentiviral RNA is one alternative...prep as the measurement will include defective particles. Studies have found that values can be 10-1000...quantify virus as it only measures infectious viral particles. One of the si mplest methods of measuring functional...distinguish between a cell that has had one viral particle integrated into its genome and a cell that has...

...a lot of time in PubMed searching for research articles? Well then, my scientist friend, do I have a handy...few quick steps, you can use PubMed to see which articles contain plasmids deposited at Addgene from the... Plasmids” badge, you’ll know that within that article you’ll find new plasmids. Simply click on the badge...site (Side note: I always recommend you read the article to ensure you understand the features of the plasmids...log-in to your PubMed account and are browsing article abstracts you should see the “Find Plasmids” box...the Literature How to Write a Scientific Review Article Tips for Improving Your Next Manuscript Resources...

...to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles in non-... on the average number of citations for recent articles. Of course, a publication in Science or Nature... citation counts for a moment, it’s clear that articles in less-prestigious journals have been gaining...Journals” found that the proportion of highly cited articles appearing in lower-prestige journals has risen...impact of research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649    ...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...MarathonRT: A highly processive reverse transcriptase Article contributed by Will Arnold  Listen to the MarathonRT...monitor retrograde transport at the cell surface Article contributed by Marcy Patrick  Listen to the nanobody...proteins occupying a specific genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex...proteins occupying a specific genomic locus: C-BERST Article contributed by Klaus Wanisch  Listen to the C-...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...here.  Human whole genome sgRNA iBAR library Article contributed by Susanna Bachle Figure 1: Initial...Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein Sparse Predictive...GFP reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a ...TALE-fluorescent protein fusions as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double...

...of virus, not the individual viral particles. A single viral particle is called a virion. Finally, viral...they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not just ...many After a virion infects a cell, the single particle creates many copies of itself, sometimes up to...eliminate their replicative ability. The remaining particle, which retains the protein coat (and envelope)...genetic payload to a cell but cannot create new particles. As a bonus, this increases the carrying capacity...

...will ensure the virus gets produced, and viral particles are packaged, when the vector is expressed. Once...expressed within the production cell line, viral particles will be produced and packaged, resulting in functional...functional virions. These viral particles can infect other cells, but since the majority of the viral ...be collected for the transduction step. Viral particles are secreted into the cellular media, making viral...like to infect with the virus. Then the viral particles do the rest! Downstream expression or integration...1: Transduction to expression process. A viral particle enters a host cell, enters the host nucleus, is...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...microtubule-associated protein to regulate cell growth dynamics Article contributed by Klaus Wanisch  Listen to the optogenetics...for visualizing RNA production and localization Article contributed by Tyler Ford  Listen to the riboglow...tools for developing new FPs - the mCarmine story Article contributed by Caroline LaManna  Listen to the ...quantification of base-editing and Cas9 cleavage activity Article contributed by Angela Kaczmarczyk  Listen to the...

...   Articles with and without plasmids at Addgene To find out whether scientific articles associated...bibliographic information and uses its vast network of articles to track the total number of citations the paper... of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations So what...estimates based on the journal that published the articles.  That is, we re-estimate the citations to the...weights. Table 1: Journal Weighting Example Article Number (A) Year Published (B) Number of Citations...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...   A retroviral mouse CRISPR knockout library Article contributed by Alycia Bittner CRISPR pooled libraries...reporter for measuring ApoB bound lipoproteins Article contributed by Leah Schwiesow A leading cause of...transcription for CRISPR activation in bacteria Article contributed by Will Arnold Baojun Wang’s lab recently...Genetically encoded voltage indicators for use in worms Article contributed by Brook Pyhtila Genetically encoded...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...efficient RNAi construct for C. elegans gene knockdown Article contributed by Alyssa Cecchetelli  Listen to the... proximity labeling with TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID.... Changing the way we detect viral outbreaks Article contributed by Izzy Mueller  Listen to the viral...collection reveals novel peptide ligand motifs Article contributed by Michael Lemieux  Listen to the SH3...

...to as hosts) via viral particles, the functional infection unit. Each particle has a viral envelope, which...the target cell’s membrane, allowing the viral particle access into the cell. The viral envelope and its...cargo – its own genome.   Fig. 1: Viral particle infection into a host cell. Image courtesy of ... measures, like replication incompetent viral particles, are required. We will be covering these safety...localization signals which can traffic their viral particles inside the nucleus, without the need for envelope...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-...using the fluorescent voltage indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage...studies easier with a 2in1 vector cloning system Article contributed by Michelle Cronin  Listen to the 2in1...tools for photoswitchable proteins cPYP and AsLOV2 Article contributed by Angela Abitua  Listen to the photoswitchable...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...here.        AAV delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great ...fluorescent genetically encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded...: 31685893. A MoClo toolkit for cyanobacteria Article contributed by Andrew Hempstead The Modular Cloning...optogenetic tool for precision in cell lineage tracing Article contributed by Erin Sanders CreLite, designed by...

...Didier Trono's lab webpage explains how lentiviral particles are produced by co-transfecting three essential...envelope glycoprotein will be expressed on virus particles? A5: This depends on the envelope plasmid you ...common due to its broad host range, and increased particle stability; however, the protein is cytotoxic precluding...target cells, but cannot produce any new viral particles after the initial infection. SIN is shorthand ...all the components necessary to produce viral particles. The components are divided as follows: Transfer...Meghan Rego contributed to the writing of this article. Additional Resources: Didier Trono Lab: Lentivectors...

...transfected into target cells which produce lentiviral particles.   Why pseudotype? Lentiviruses and retroviruses...4 lab. For more details, check out this review article published in Reviews in Medical Virology (Li et... proteins have been pseudotyped on lentiviral particles. Rabies glycoprotein The rabies glycoprotein targets...suit your needs. Check out some of these review articles to find tables and information on different viral...Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization...Jr (2000) VSV-G Pseudotyped Lentiviral Vector Particles Produced in Human Cells Are Inactivated by Human...

...lab used SNAP-tag to decorate synthetic viral particles with BG-linked antibodies to drive cell type-specific...intracellular targets. Nature Communications, 7(1), Article 1. https://doi.org/10.1038/ncomms10778 Erdmann,...molecules in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765 Lavis, L. (2016...A flexible chemical tag. Nature Methods, 2(4), Article 4. https://doi.org/10.1038/nmeth749 Strebinger,...envelope design. Nature Communications, 14(1), Article 1. https://doi.org/10.1038/s41467-023-40788-8 Ulrichs...capping protein. Nature Communications, 14(1), Article 1. https://doi.org/10.1038/s41467-023-39655-3 Wilhelm...

... Chromatin research articles with plasmids available from Addgene Many articles reporting chromatin-related...plasmids from hundreds of articles in our collection; the following articles are just a small cross-section...jumping off point. The following lists of PIs, articles, genes, and blog posts are not comprehensive, ...