...immobilization for kinetic analysis Proteolytic removal of the fusion protein could provide a more relevant...second immobilization method. One possibility is covalent modification of the purified target protein with...and neutravidin) is for all practical purposes covalent. Therefore, protein immobilization through biotin-avidin...amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids. Unfortunately...

...technology (Yesbolatova et al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods...tagging of proteins with mAID and other tags (Yesbolatova et al., 2019) using CRISPR. This toolkit makes...potential of mining datasets for research and innovation. Find MerMAIDs plasmids at Addgene Opperman J...

...new resource from Jennifer Doudna’s lab at the Innovative Genomics Institute. What is CasPEDIA? CasPEDIA...and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving database, and...Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019)...CRISPR Beyond SpCas9 More resources from the Innovative Genomics Institute      CRISPRpedia IGI’s ...

...switching via a novel optogenetic variant of AcrIIA4 (CASANOVA). As shown in the figure below, in the absence...release of Cas9. A noteworthy application of the CASANOVA system was its use in light-mediated CRISPR labeling...minutes and red foci formed 20-40 min later. The CASANOVA system is versatile in that it can be adapted ...encoded photocaging technique to insert a light-removable protecting group, specifically a nitrobenzyl photocaged...Light-Dependent Anti-CRISPR Bubeck et al., 2018 CASANOVA LOV2 from A. Sativa AcrIIA4 anti-CRISPR protein...

...phosphopantetheinyltransferase) – These enzymes catalyze the covalent attachment of a fluorophore ligand to a peptide...ligands. New fluorophores As a corollary to these innovative labeling strategies, several groups—including...infusion of photons for your imaging experiment. Innovative labeling strategies and improved fluorophores...and manipulating proteins in live cells through covalent labeling." Nature chemical biology 11.12 (2015...

...Biotin is a cofactor that in its active form is covalently attached to the active site of key metabolic ...in cells and is the process by which biotin is covalently bonded to a specific lysine at the active site...the bond between them is one of the strongest noncovalent protein-ligand interactions found in nature. ...in live mammalian cells with biotin ligase and monovalent streptavidin.” Nature Protocols 3.3 (2008): 534...

...modification system and the scarless, Footprint-Free™ removal of cargo with the excision-only piggyBac™ transposase...stimulate homologous recombination and Footprint-Free™ removal of the selection cassette by excision-only piggyBac...site is left behind in the genome following donor removal, which alters the endogenous DNA sequence and has...nuclease thereby preventing re-cutting. Transposase removal of the cassette later restores the target sequence...

...reagents If you want to label intracellular proteins covalently with FRET acceptors, then biarsenicals are a ...The utility of these reagents is that binding is covalent, so free dye can be washed away. In addition, ...experimental design lends itself to creativity and innovation, valuable commodities in any NIH grant application...Stephen R. Adams, and Roger Y. Tsien. "Specific covalent labeling of recombinant protein molecules inside...

...did we get here in the first place? How CRISPR innovation affected software development CRISPR, like the...it, offers an interesting view into how rapid innovation affects the research community. I’ve likened ...That’s just 3 to 4 years ago. It is a bubble of innovation (a piñata), and software development has caught...future of CRISPR software is promising. Rapid innovation in this space has put us in a good position to...

...This time: E. coli genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base ...E. coli genome-wide CRISPR inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed...single-stranded oligodeoxynucleotide HDR template covalently attached to the Cas9-gRNA RNP complex via the...

... wanted to be able to build assemblies through covalent bonds so that the assemblies wouldn’t fall apart...build a highly multivalent assembly connected by covalent bonds, so there were no weak links in the chain...2014 Mar 17. PubMed) Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial...

...fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that HcKCR1 and...photoactivation of HcKCR1. Images adapted from Govorunova et al. 2022.     HcKCRs represent a long-...performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins are natural...

... of bacterial genomes utilize this system (4). Makarova et al. grouped the systems into three distinct...programmable platform well-suited for various innovative biological and translative technologies. Addgene...1709–1712 (2007). CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier...

...was the Head of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development...are less common, they can be very impactful. At Novartis, Stegmeier deposited the ClonTracer Barcoding ...built create inefficiencies that ultimately make innovation and science slow,” Vandenberghe says. “The continuous...

.... The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand and a tyrosyl...different DNA strand comes along, it can attack this covalent bond thus joining the two DNA strands and releasing...overhanging 3´ deoxythymidine (T) residues that are covalently linked to topoisomerase. Vectors in these kits...

...Schwiesow A leading cause of death worldwide, cardiovascular disease is driven by atherogenic lipoproteins...been shown to have a strong correlation with cardiovascular disease and indirect measurement of ApoB-LPs...to aid in the understanding and treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene...

...activity after ischemic brain injury Dr. Gohar Tsakanova, Institute of Molecular Biology of the NationalAcademy...Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular...two-photon laser scanning microscope will allow Tsakanova to image cortical neurons and study changes in...

...She is particularly interested in exploring how innovative tools influence the direction of scientific research...    Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT Sloan School of Management...performance, and randomized control trials in innovation. Visit his website at: http://www.neil-t.com/...

...powerful tool in almost all disciplines. In biology, innovative new imaging approaches are constantly being developed...and disciplinary boundaries and can allow for innovative organizations and companies to provide problem-solving...s41467-020-19447-9 Hohlbein, J., Diederich, B., Marsikova, B. et al. Open microscopy in the life sciences...

...Perez-Pinera et al., 2013). In the years that followed, innovative methods greatly improved CRISPRa, expanding its...E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon...Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J,...