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We narrowed to 639 results for: Rest

Showing: 21 - 40 of 639 results

  1. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...solubility tag GB1. In SunTag nomenclature throughout the rest of this blog post, GFP refers to sfGFP-GB1.   Tanenbaum...fusing a fluorescent protein to your protein of interest, you instead fuse it to the synthetic SunTag scaffold...

  2. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...comprehensive pre-compiled downloads and via a full-featured REST Web Service API. Both options are fully open access...CRISPR results for any gene of interest. In addition, users interested in a particular disease, cell type...literature for a drug, disease, or condition of interest using a simple keyword search. For example, searching...entire CRISPR database for particular screens of interest.   Figure 3: Each publication featured ...access and available without any licensing restrictions.    As CRISPR technology continues to develop and...

  3. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ... cells that contain a specific gene survive. The rest do not grow. While there are many ways to design...You’ve worked hard to purify your gene of interest, get it into your plasmid backbone, and zap the mixture...some now have plasmids that contain your gene of interest, but others will uptake plasmid backbones that...antibiotic resistance cassette along with your gene of interest. In these cases, antibiotic selection will select... if the plasmid backbone contains your gene of interest. Auxotrophy Besides antibiotic selection, you ... select for a successful uptake of your DNA of interest by taking advantage of bacterial characteristics...by recombination sites. To introduce a gene of interest into the destination plasmid, recombination sites...

  5. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...system. The effector is placed downstream of the rest of the SPARC components and expression is controlled...used to specifically manipulate a cell type of interest in an organism, all cells of that type are affected...affected. Previous tools to overcome this restraint include an AAV-based sparse labeling system where a...

  6. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...CRISPR-Cas nucleases, provided important context for the rest of the day. He was followed by three of the biggest... weren't quite sure what to expect in terms of interest, but visitors to the poster were impressed to ...edge science in increasingly important field.  Interested in Addgene's Keystone Poster?  We'll be sharing...

  7. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...from the rest of our panel. Lastly, we want to detect the expression of our protein of interest: CD45. ...It also means we should exclude APC-Cy7 from the rest of our panel. Besides some background cell death...through genetic modification. As your protein of interest will only occur in combination with that reporter...antibody-fluorophore combinations for different markers of interest, you can separately detect these markers in your...

  8. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... now you don’t have to worry about operating the rest of the car as well. Similarly, cell free expression...keeping. Finally, if you are a fellow researcher interested in adapting your research ideas into hands-on...student at MIT in Biological Engineering and is interested in developing synthetic biology based tools for...

  9. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...FPbase data in their own projects and applications, a REST API allows programmatic retrieval of the majority...that serve to further characterize a protein of interest (and we very much appreciate even those small ...

  11. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ... with the virus. Then the viral particles do the rest! Downstream expression or integration resulting ...constructed viral vector containing the cargo of interest is then typically transfected along with viral...

  12. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...This variant exhibits high contrast between its resting state and its calcium-bound state, which was achieved...

  13. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...just go to sessions that you find interesting and not go to the rest.” Having an on-site conference canceled...started yet! @neurosymp will be interesting this year. Everyone interested in #Neuroscience should come ...aspect and I could plan more with regard to my interests.” Use the chat box and upvote If the thought of...

  14. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the Flowtime Technique...

  15. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...the PCR product Isolate your PCR product from the rest of the PCR reaction using a kit, such as the QIAquick...assist with restriction enzyme digestion (usually 3-6bp) Restriction Site: Your chosen restriction site for...plasmid of interest. You can use similar processes to add overhangs to your insert of interest for Gibson...fusing the upstream restriction site to the forward primer and the downstream restriction site to the reverse...discussed in our restriction cloning post. However, even more than with standard restriction cloning, it is...of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that...itself are very similar to those outlined in our restriction cloning post with a few quirks specific to the...

  16. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists Series Learn How... careful not to go too far. Is the assignee interested in this task? Don’t give out tasks – where possible...

  19. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...the meeting and then communicate outcomes to the rest of their team. Here's a template for project meetings...

  20. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...and techniques. If you are interested in reading more, you can find the rest of the Plasmids 101 posts ...candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli ...
Showing: 21 - 40 of 639 results