We narrowed to 29 results for: SKIP

1. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   Published

   Dec. 2, 2014, 7:23 p.m.

   ...engineered DNA-binding molecules even enables us to skip the step of knocking-in. In 2013, we showed that...

2. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ...peptides are thought to function by making the ribosome skip the synthesis of a peptide bond at the C-terminus...

3. Negotiating Work and Life: How to Find the Joy

   Type

   Blog Post

   Published

   March 20, 2014, 3:04 p.m.

   ...put this in print, but I actually let my daughter skip school a couple of times. Be sure to add time with...

4. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ...Drosha to pre-miRNA. miRNA plasmids are engineered to skip this first pri-miRNA step. These RNA duplexes bind...

5. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   Published

   Feb. 18, 2016, 3:42 p.m.

   ...wide gel comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder...

6. RNA Interference in Plant Biology: New Tools for an Old Favorite

   Type

   Blog Post

   Published

   Oct. 27, 2020, 1:15 p.m.

   ...maintain partial function due to the use of exon skipping, alternative start codons, and/or the phenomenon...

7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   Published

   Nov. 2, 2021, 1:15 p.m.

   ...a fair amount of time, and it may be tempting to skip some steps and get to experiments. However, by optimizing...

8. Deep Dive: qPCR

   Type

   Blog Post

   Published

   Sept. 29, 2022, 1:15 p.m.

   ...so pick one and use it! Tempting as it may be to skip this step, remember we here at Addgene frown upon...

9. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

   Type

   Blog Post

   Published

   Jan. 13, 2025, 9:49 p.m.

   ...complementary edits to opposite strands of the DNA and skip the cellular mismatch repair step (Anzalone et al...