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We narrowed to 340 results for: abo.4

Showing: 21 - 40 of 340 results

  1. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...codelivery efficiency at different doses. See Figure 4 in this paper for more information.   Q6 & Q7: We ...organism such as mice, e.g. first at 2 months, then at 4 months? Or do antibodies become a prohibitive problem...: CAP-B10 and CAP-B22 were tested individually in 4 marmosets each. Brain potency for each individual ...labeling in the brain with PHP.eB: A recent CLOVER collaboration injected 1e+13 vg per mouse to get phenotypes...coverage. Please see this paper for more information about PHP.eB brain labeling at various doses.  As always...

  2. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...small molecule derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). DFHBI is... ligands by exponential enrichment (SELEX). After 4-6 rounds of SELEX, the RNA pool was cloned into bacterial... dimer activates the fluorescence of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a...fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908. PubMed Central ...occurring regions in the 5’ UTR of mRNA that bind metabolites, leading to conformational changes that control...Spinach-based riboswitches can be used as stable metabolite sensors for imaging in live cells. By placing...placing Spinach downstream of a riboswitch, metabolite binding to the riboswitch instead activates Spinach ...

  3. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... formation of plasmid multimers. Cell. 1976 Dec;9(4 PT 2):707-16. doi: 10.1016/0092-8674(76)90134-3. PMID...in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13. doi: 10.1073/pnas.61.4.1406. PMID: 4884687...capillary gel electrophoresis. BioProcess Int. 2006 Sep;4(8):38-40. Summers D. Timing, self-control and a sense...plasmid monomerization and stability. Cell. 1984 Apr;36(4):1097-103. doi: 10.1016/0092-8674(84)90060-6. PMID...upstream production. Biotechnol Adv. 2009 Jul-Aug;27(4):353-70. doi: 10.1016/j.biotechadv.2009.02.003. Epub...colonies using one of the detection methods described above can help ensure the isolation of the monomeric version...plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes...

  4. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...expression. When using these constructs to tag the GOLG-4 protein, mScarlet3 was 50% brighter than the original... Figure 3: Expression of fluorophore-tagged GOLG-4 through eight stages of embryonic development. Representative...knockouts limits further cutting and errors (Figure 4). This work marks the first use of CRISPR/Cas9 in ...pathogenicity, and resistance mechanisms.     Figure 4. Overview of the dual-plasmid CRISPR/Cas9 workflow...more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...stepwise, achieving mutation efficiencies of up to about 9% of colonies (102 to 104 times higher than traditional...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS...

  5. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...2in1 Plasmid Toolkit consists of 4 plasmid toolkits generated with 4 different, binary 2in1 vectors with...combinations of tag orientation (NN, NC, CN, and CC). The 4 kits also contain the two entry vectors pUC-L1L4 and... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...were observed binding telomeric DNA. What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9...

  6. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969-4.   Increased neuronal expression using Cre-mediated...Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969-4.   dCas9-SSAP with reduced error rate for long sequence...to be deconvoluted using freeware.    Fig. 4: Images of 12 visual barcodes in use in A375 cell ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. ...

  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...board, Jacqueline N Crawley  [et al]. 2013;4(435):4 35 1-4  20. doi: 10.1002/0471142301.ns0435s65. PMID: ... different variants [1, 2, 4]. And, of course, the best way to learn about the many different opsin variants...animal models. Nature reviews Neuroscience. 2012;13(4):251-66. doi: 10.1038/nrn3171. PMID: 22430017. 2. ....2011.10.003.  PMID: 22055387;  PMCID: PMC3225502. 4. Mattis J, et al. Principles for applying optogenetic... electrophysiology data too but that’s what collaborators are for). For example, if you study neural pathways...intended brain region and/or cell type (I’ll talk more about this in detail later). But what are the different...of your opsin, but keep you eyes open for posts about the next steps in the coming weeks. Expression of...

  8. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...vacuole and emit red and green fluorescence. After 4 hours of nitrogen starvation to induce autophagy, ...turnover of cytosol and organelles in yeast. Autophagy,4(2), 205-213. doi:10.4161/auto.5331. PubMed PMID: 18094608...PMID: 20144757. PubMed Central PMCID: PMC2852113. 4. Klionsky, D. J., Cuervo, A. M., & Seglen, P. O. (...information about the contents of the autophagosome nor does it provide information about what is being...higher pH of the vacuole. Read on to learn more about prior methods for studying autophagy and how Rosella... (as opposed to giving more fine-grained detail about what proteins or organelles are being degraded) ...autophagy, but, like the protein degradation assay above, it’s slow and only measures bulk autophagy. Morphological...

  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...alpha L [IPI-TS2/4] Integrin alpha-L Human IgG1 Rabbit Anti-Integrin alpha L [IPI-M17/4] Integrin alpha-L...alpha L [IPI-TS2/4] Integrin alpha-L Human IgG2a Mouse Anti-Integrin alpha L [IPI-M17/4] Integrin alpha-L...roles of individual integrins. The Ha lab — in collaboration with the Springer lab, the Jaumouillé lab, the...recombinant antibodies and plasmids. To encourage collaboration and education in protein science, IPI hosts ...events and training courses on technologies and laboratory techniques related to antibody discovery. You...the Institute for Protein Innovation. She writes about all things IPI, from the scientists and scientific...

  10. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...component plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating the ...the vector is to your liking, your last step, Step 4, is to give the assembled plasmid a name and generate...assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/j.xgen.2024.100519...Destination vector (dropdown options depending on above selections) Module set (the set of components you...vectors) Target type (drop down options depending on above selections.) CRISPR enzyme (Cas9 [sp], Cas9 [other...

  11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...assembles the final targeting vector (Fig. 1, Step 4), and co-injection of the targeting vector and a Cas9...Modification in Caenorhabditis elegans.” Genetics. 202(4) (2016):1277-88. PubMed PMID: 26837755. PubMed Central...modular base-specific gene tagging using a ligase-4-dependent mechanism.” Nat Commun. 7 (2016):12338. ...tagged gene. Most insertions can only be detected by labor-intensive processes like PCR or by evaluating visual...repair after a CRISPR/Cas9 induced break. Learn more about these constructs and the cell lines they’ve been...Additional Resources on the Addgene Blog Learn about Fluorescent Protein Timers Check out Photoactivatable...

  12. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ... ShadowR, a novel FLIM-FRET acceptor Figure 4: ShadowR is used as a FRET acceptor to mRuby2 or mScarlet...energy transfer (FLIM-FRET) in living cells (Figure 4). They created ShadowR by modifying the surface of...Reports, 9(1). https://doi.org/10.1038/s41598-019-48604-4 Salih A, Larkum A, Cox G, Kühl M, Hoegh-Guldberg O... Fluorescent Protein 101 blog series Learn more about FRET Find protocols and tips for making plasmid ...

  13. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...research more inclusive. CBE Life Sciences Education. 13(4):602-606. Brian, D. 1996, Einstein: A Life. Chapter...attitudes in CUREs. CBE Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko, J., Keene, A. et al. 2022...and neurobiology. CBE Life Sciences Education. 15(4):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship...in a year-long biochemistry laboratory : Part II – research-based laboratory – a semester-long research...are often associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein...had the opportunity to talk with Provost recently about his development of what is now commonly termed a...into one of my own courses, I was excited to hear about how he developed his program. Initially, Provost...

  14. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...system. Knowing that GFP’s  structure stabilizes a 4-hydroxybenzylidene imidazolinone (HBI)-like compound...same thing? Using the HBI derivative 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) with relatively...PMID: 26000751. PubMed Central PMCID: PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology...target metabolite creates an aptamer that will only bind to DFHBI in the presence of that metabolite (Fig...approach less sensitive in most applications. Learn About Fluorescent Biosensors Light-up aptamers (or, fluorescing...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...Tips for Using FRET in Your Experiments Learn more about GFP Controlling Protein Activity with GFP Resources...

  15. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...of the 2A sequence and the next peptide downstream.4 The "cleavage" occurs between the Glycine and Proline...20(8):845-60. doi: 10.1089/hum.2008.188.) PubMed. 4. High cleavage efficiency of a 2A peptide derived ..., zebrafish and mice. Kim et al (PLoS One. 2011;6(4):e18556. doi: 10.1371/journal.pone.0018556. Epub 2011...genes above with your gene or genes of interest. Some of the plasmids listed in the table above (and their... an internal region of the mRNA. In the figure above, you can see that the IRES element acts as another... first discovered in picornaviruses, are short (about 20 amino acids) and produce equimolar levels of ...

  16. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ... and Verkhusha (Nat Methods. 2013 Aug; 10(8): 751-4. doi:10.1038/nmeth.2521) Far-red light photoactivatable...fluorescent proteins. Krumholz et al (Sci Rep. 2014 Feb 3; 4:3939. doi: 10.1038/srep03939.)  ... absorbance of water, which absorbs wavelengths above 900 nm or so. In other words, wavelengths between...

  17. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...periods of time, especially at room temperature or at 4°C. Storing DNA: temperature and longevity You should...be stable for years. Plasmid DNA can be stable at 4°C or even room temperature for a short period, and...to purify DNA without a kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts...

  18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused an estimated total of 380,000...deaths worldwide (statistics provided by Google on 6/4/20). Many countries entered lockdown for weeks to ...during weekends and significant holidays. Figure 4: Plasmid request trajectories suggest an exponential...in his laboratory. Learn more here. Additional resources on the Addgene blog Learn more about plasmid...

  19. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ... for more than making pretty pictures (see figure 4). It also enables single-cell anatomical study and...49 +/- 7% 36 +/- 8% 37 +/- 10% AAV.PHP.eB 69 +/- 4% 55 +/- 7% 76 +/- 5% Table 1: Transduction efficiency...induction of multi-color labeling of neurons (figure 4). These capsids can serve as powerful cell-type-restricted...or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they can be useful...Important considerations when using AAVs Learn more about using AAV as a tool for gene expression in mammals...

  20. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable...methods. 2009. May;6(5):343–345. Pubmed PMID: 19363495. 4. Josse J, Kaiser AD, Kornberg A. Enzymatic synthesis...Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. PubMed PMID: 25909630. PubMed Central PMCID...Resources on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids...
Showing: 21 - 40 of 340 results