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We narrowed to 151 results for: cycs

Showing: 21 - 40 of 151 results

  1. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...2. Viral stocks may be sensitive to freeze thaw cycles. Depending on the researcher and the type of virus... of titer losses from 5% to 50% per freeze thaw cycle (Krajden et al., 1999;  Ugai et al., 2002). If the...However, polybrene is highly sensitive to freeze-thaw cycles and should be stored in single-use aliquots. Never...Krajden, Mel, et al. "Effect of multiple freeze-thaw cycles on hepatitis B virus DNA and hepatitis C virus ...

  2. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ... vector. In the absence of tetracycline or a derivative such as Doxycycline (Dox), the Tet-controlled ...needs to be expressed a certain point in the cell cycle, a transfer vector with an inducible promoter (see...used inducible lentiviral vector system is the tetracycline (Tet)-regulated system. You can choose either...

  3. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...other posts on lab sustainability, check out my Recycling and Innovations posts. Making small changes in...sustainability project. This could be: Setting up a recycling program at your institute. Set your -80 °C freezer...Additional resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene...

  4. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal...genome editing system that could be regulated by doxycycline. Now, they’ve developed another AAV toolkit -...system is regulated not only temporally with doxycycline, but it can also be regulated spatially with ...

  5. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...creating less waste.  Most communities seem to have recycling programs and it seems to me that most Addgenies...fewer people use plastic bags.  We also try to recycle our discards according to the Cambridge, Massachusetts...chipped or imperfect equipment.  We can’t safely recycle these materials either.  Test tubes aren’t cheap...

  6. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...repeated cycles will favor accumulation of the latter products. As noted above, a single cycle of cleavage... Unlike HDR, NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is...

  7. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...these components as part of its natural viral life-cycle, and researchers have adapted the components for...recombination” (although later referred to as the “cyclization recombinase”), is a 38 kDa protein responsible...the Cre inducible (such as with tamoxifen or doxycycline), the Cre recombinase can be expressed only in...

  8. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...there are many places where the PhD application cycle becomes much more complex. “How do I reach out to...respond to your email. During my PhD application cycle, I reached out to around twelve professors and heard...like that! For instance, during my application cycle, two schools on opposite sides of the country had...

  9. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...added to the mastermix that are read after each cycle. The raw data is then normalized to a reference ...dependent on number of samples rather than number of cycles.  Choosing a method  As with all experiments, a...analysis of copy number variants based on real-time LightCycler PCR. Curr Protoc Hum Genet. 2014;80:7.21.1-7.21...

  10. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...exist. The life cycle of AdVs is dependent on modulation of the host cell’s cell cycle; inhibition of ...., 2003).        Figure 1: The adenovirus life cycle. Image reused from Kremer and Nemerow 2015 under...

  11. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...stimulate quiescence, the resting phase of the cell cycle; quiescent cells do not take up nucleic acid or ...lentiviral transduction. The effect of freeze-thaw cycles on lentiviral stability depends on a variety of...produced at pH 6 are resistant to multiple freeze thaw cycles. Consequently, it is best to avoid freeze-thawing...

  12. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...not recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG...T7RNAP-tetA General protein expression  BLR (DE3) Tetracycline RecA-deficient; best for plasmids with repetative...proteins Origami2 (DE3)** Streptomycin and Tetracycline Contains highly active thioredoxin reductase...

  13. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP." Journal of the American Chemical...et al., 2017) and levels of the second messenger cyclic di-AMP in Gram-positive bacteria (Kellenberger ...

  14. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... streptomycin Tn10   Confers resistance to tetracycline Additionally, Table 2 provides a quick reference...galE15 galK16 rpsL(StrR) endA1 λ- XL1 Blue Tetracycline Blue/white screening and routine cloning, nalidixic...lacIq Δ(lacZ)M15] hsdR17(rK- mK+)  XL10 Gold Tetracycline and Chloramphenicol High competency cloning ...

  15. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ... against the Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene...expression by administering an inducing agent like doxycycline (Favre et al., 2002). They are useful when you...

  16. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ... model organism for studying the bacterial cell cycle, asymmetric cell division, and cellular differentiation...repressed ctrA, a gene encoding an essential cell cycle regulator. The lab expressed both dCas9 and the ...

  17. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...dynamics, and how they change in response to the cell cycle or other stimuli.   To create a colorful Cas9 toolbox...technique will be applicable to studies of cell cycle progression, epigenetics and cellular reactions ...

  18. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...and Paul Greengard. "Dopamine-sensitive adenyl cyclase: possible role in synaptic transmission." Science... Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its similarity...

  19. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...techniques: unwanted mutations due to excessive PCR cycles or low fidelity Taq DNA polymerase, the construction...high fidelity DNA polymerases like phusion. An 18-cycle PCR leads to the most colonies after transformation...

  20. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ... of XFPs in the original method, we used the Tetracycline trans-activator system to boost the expression...expression levels, and we named the system Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al.,...
Showing: 21 - 40 of 151 results