pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
Type
Blog Post
Published
Dec. 14, 2017, 2:08 p.m.
...replication, OriP and the EBNA-1 sequence that encodes for the DNA binding protein EBNA-1 [7]. These two sequences...are based on the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA-1) system where each vector contains a viral ...extra plasmid, pCXWB-EBNA-1 is used in the reprogramming cocktail. pCXWB-EBNA-1 lacks oriP and is unable...expressing additional EBNA-1 transiently. The transiently expressed EBNA-1 increases protein expression...pCXLE vectors listed below in Table 1 are available at Addgene.
Table 1: List of pCXLE episomal plasmids,...obtained and the subsequent reprogramming success [1].
Fibroblasts need to be cultured and expanded in ...reprogramming factor cocktail, and viral protein EBNA-1.
Methods for reprogramming hiPSCs from PBMCs
While...