We narrowed to 131 results for: itch

...overexpressed in cells, microtubule growth can be switched off with blue light which causes LOV2 and Zdk1...cells. These tools consist of cobalamin-binding riboswitches that bind to cobalamin-fused fluorescent dyes...dyes; binding of the cobalamin fusion to the riboswitch results in increased fluorescent signal. Prior ...find plasmids containing the cobalamin binding riboswitches used in the Riboglow platform at Addgene. ...

...your idea is viable.  Go to experts you trust and pitch what you hope to build.  Then ask for their honest...honest opinions -- this part might hurt, but if you pitch to enough people, you will find the answer to your...an impossibility at our early stage.  After each pitch we made, we considered the feedback and adjusted...viable.  This is not to say that we aren’t still pitching for feedback.  Your venture should continuously...

...derivatives of violacein. Using dCas9 as an on/off switch The multiple scRNAs all individually utilize the... can be regulated with dCas9 as a master ON/OFF switch. This will likely prove particularly useful in ...distinct programs controlled by each dCas9 regulatory switch. By no means limited to the field of metabolic ...variants can be used as regulatory ON/OFF master switches to induce multiple transcriptional programs. ...

...Xin Zhou greatly expanded that list by using photoswitchable fluorescent proteins to create enzymes that...turned the enzymes back on. Xin termed these photoswitchable enzymes Fluorescent Light-inducible Proteins...various designs, Xin has been able to develop photoswitchable Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5,... You can learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in ...

...infection. Cre or Flp dependent switches control gene expression via switches placed in your cell type of ...specialized promoters, Flp or Cre dependent gene switches, and serotype selection. Promoters and serotype...interest. Careful combination of promoters, gene switches, and/or serotype can also be used to ensure specificity...

...function. Adapting Toehold Switches to Detect Zika Virus The RNA-based toehold switches described in this post...binding, produce reporter proteins. These toehold switches can be used to quickly detect the presence of ...

... discussion on the way to the office or in the kitchen, while sipping an afternoon coffee in between experiments...a coffee, or simply moves away from the desk to switch off a few minutes. The host could also set aside...used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas. Social ...

...beyond stability, adding color and even on-off switches. Stabilizing gRNA modifications  Sugar phosphate...modifications to improve stability.   On-off switches for gRNAs A major drawback of CRISPR-Cas9 technology..., E., Satyapertiwi, D., Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based...

...subclass specific secondary antibodies and subclassed switched R-mAbs, multiple targets could now be visualized...Fig. 2. Multiplex immunolabeling with subclass-switched recombinant antibodies in adult rat brain. Figure...Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...

...to control cells or organisms with the flip of a switch. You may be familiar with popular light-sensitive...activity. Researchers have designed a variety of photoswitchable elements into many other types of proteins....in the Gadella Lab devised a set of optogenetic switches for the GEFs ITSN1, TIAM1, or p63RhoGEF (which...

...were a way to just make one fusion protein that switches to any color or tag you want? It sounds like magic...ticker tape" method for intracellular recordings, switching between different colored ligands at different...increasingly popular. Embrace the flexibility and switch things up for your next experiments!   References...

... or activity can be achieved using switches, including riboswitches and optogenetic and chemogenetic tools...tissue or cell type specificity. An on/off genetic switch can be used for expression control. (c) Viral delivery...Dual expression Promoters, miRNAs, enhancers, and switches all take up precious cargo space in AAV vectors...

...Other ways to make the office more eco-friendly The switch to 100% renewable electricity is certainly not ...sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple streams for composting...

...advance your career, promote career growth, and/or switch your field of work, both in academia and in industry...specialized so it might lend itself more readily to a switch. 4. Do you want more autonomy?  Before my master...

...depolarization. They therefore function as a neuron “on switch.” Neuronal inhibition: Hyperpolarization Conversely...the cell. It therefore functions as a neuron “off switch.” Because of the differing peak absorption wavelengths...

...activates transcription with the flick of a light switch and shuts off just as quickly. The original AraC...bacteriograph was created using 160 individual images stitched together. Image from Romano et al., 2021 with ...

...zebrafish embryo over an 80 minute time course.  By stitching together a stable scaffold and HA-binding regions...protein dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and...

...articles. Think we missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps...Andrews NP,  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...

...we’ll discuss inducible promoters, which can be switched from an OFF to an ON state, and how you might ...The Promoter Region Plasmids 101: Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other...

...everything is conceptual, and the team that packages its pitch the best, wins. He explained that entrepreneurs ...the team works well, the idea is solid, and “the pitch is packaged to perfection”, seed money will hopefully...