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We narrowed to 129 results for: itch

Showing: 21 - 40 of 129 results

  1. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...your idea is viable.  Go to experts you trust and pitch what you hope to build.  Then ask for their honest...honest opinions -- this part might hurt, but if you pitch to enough people, you will find the answer to your...an impossibility at our early stage.  After each pitch we made, we considered the feedback and adjusted...viable.  This is not to say that we aren’t still pitching for feedback.  Your venture should continuously...

  2. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...derivatives of violacein. Using dCas9 as an on/off switch The multiple scRNAs all individually utilize the... can be regulated with dCas9 as a master ON/OFF switch. This will likely prove particularly useful in ...distinct programs controlled by each dCas9 regulatory switch. By no means limited to the field of metabolic ...variants can be used as regulatory ON/OFF master switches to induce multiple transcriptional programs. ...

  3. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...Xin Zhou greatly expanded that list by using photoswitchable fluorescent proteins to create enzymes that...turned the enzymes back on. Xin termed these photoswitchable enzymes Fluorescent Light-inducible Proteins...various designs, Xin has been able to develop photoswitchable Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5,... You can learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in ...

  4. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...infection. Cre or Flp dependent switches control gene expression via switches placed in your cell type of ...specialized promoters, Flp or Cre dependent gene switches, and serotype selection. Promoters and serotype...interest. Careful combination of promoters, gene switches, and/or serotype can also be used to ensure specificity...

  5. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...function. Adapting Toehold Switches to Detect Zika Virus The RNA-based toehold switches described in this post...binding, produce reporter proteins. These toehold switches can be used to quickly detect the presence of ...

  6. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ... discussion on the way to the office or in the kitchen, while sipping an afternoon coffee in between experiments...a coffee, or simply moves away from the desk to switch off a few minutes. The host could also set aside...used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas. Social ...

  7. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...subclass specific secondary antibodies and subclassed switched R-mAbs, multiple targets could now be visualized...Fig. 2. Multiplex immunolabeling with subclass-switched recombinant antibodies in adult rat brain. Figure...Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...

  8. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...beyond stability, adding color and even on-off switches. Stabilizing gRNA modifications  Sugar phosphate...modifications to improve stability.   On-off switches for gRNAs A major drawback of CRISPR-Cas9 technology..., E., Satyapertiwi, D., Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based...

  9. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...to control cells or organisms with the flip of a switch. You may be familiar with popular light-sensitive...activity. Researchers have designed a variety of photoswitchable elements into many other types of proteins....in the Gadella Lab devised a set of optogenetic switches for the GEFs ITSN1, TIAM1, or p63RhoGEF (which...

  10. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...were a way to just make one fusion protein that switches to any color or tag you want? It sounds like magic...ticker tape” method for intracellular recordings, switching between different colored ligands at different...increasingly popular. Embrace the flexibility and switch things up for your next experiments! References...

  11. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ... or activity can be achieved using switches, including riboswitches and optogenetic and chemogenetic tools...tissue or cell type specificity. An on/off genetic switch can be used for expression control. (c) Viral delivery...Dual expression Promoters, miRNAs, enhancers, and switches all take up precious cargo space in AAV vectors...

  12. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...Other ways to make the office more eco-friendly The switch to 100% renewable electricity is certainly not ...sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple streams for composting...

  14. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...depolarization. They therefore function as a neuron “on switch.” Neuronal inhibition: Hyperpolarization Conversely...the cell. It therefore functions as a neuron “off switch.” Because of the differing peak absorption wavelengths...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...articles. Think we missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps...Andrews NP,  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...

  18. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...we’ll discuss inducible promoters, which can be switched from an OFF to an ON state, and how you might ...The Promoter Region Plasmids 101: Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other...

  20. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...At the heart of their method is the RNA toehold switch, a RNA hairpin that can be designed to bind and...interest is a benefit of this method. Find the toehold switch microbiota/biomarker sensors here Studying the ...
Showing: 21 - 40 of 129 results