We narrowed to 31 results for: mcs

...target sequence can be placed in multi-cloning site (MCS) between EGFP fragments. The pX330 plasmid contains...

...the promoter as part of a multiple cloning site (MCS). Having multiple sites allows you to easily orient...

... Addgene.  Lei Y, Zhang X, Su J, Jeong M, Gundry MC, Huang Y-H, Zhou Y, Li W, Goodell MA (2017) Targeted...

...References Canning EA, LaCosse J, Kroeper KM, Murphy MC (2019) Feeling Like an Imposter: The Effect of Perceived...

...tpc.113.112235 Morgens DW, Deans RM, Li A, Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi...

...Monogarov G, O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki M et al. 2018. An efficient ...

...primer containing the desired restriction site (the MCS has several options) and a pUCX-specific reverse ...region. The PCR product is then ligated into the MCS/SacI site in pUCXKT, removing the stop codons during...

...keratinocytes to peripheral blood mononuclear cells (PBMCs). PBMCs isolated from whole blood present many benefits...reprogramming while PBMCs collected from blood are reprogrammed immediately after collection [2]. PBMCs display unique...to derive iPSCs from PBMCs. Most laboratories use Sendai virus to reprogram PBMCs efficiently [5]. However...age-matched fibroblasts [3]. These advantages make PBMCs the ideal source of somatic cells for reprogramming... EBNA-1. Methods for reprogramming hiPSCs from PBMCs While the method of choice for reprogramming often...usage of Sendai virus. Reprogramming efficiency of PBMCs with episomal vectors Episomal vectors are based...reprogramming factors. However, reprogramming of PBMCs by episomal vectors has yielded hiPSCs at a low ...

....” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate student at Duke who has recently....  “The disease was first described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed...expressed specifically in neurons of the brain. McSweeney says they now think that mutations in the gene...

...PBMC) in 3D models of the human lymphatic system. PBMCs consist of various immune cells, and can be used...subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can be further differentiated into dentritic cells...

...populations within a heterogenous tissue type, such as PBMCs or tumors, and moreso than other methods, the identification...