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We narrowed to 336 results for: nam

Showing: 21 - 40 of 336 results

  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...sample or protein of interest to understand the dynamic or working range of a given reagent (usually an...protein concentrations are within the antibody’s dynamic range. Created with biorender.com   Alternatively...recommended dilution. If the antibody dilution’s dynamic range encompasses your protein’s expected concentrations...dilution until your protein dilutions are in the dynamic linear range (i.e., expression decreases as protein....     Figure 3: Testing the antibody’s dynamic range. A) The protein dilutions, representing expected... concentrations, are all within the antibody’s dynamic range. B) The protein dilutions, representing expected... concentrations, are outside of the antibody’s dynamic range. Specifically, the 1:2, 1:1, and No dilution...

  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... creating the SunTag system. The SunTag system, named after the "stellar explosion SUperNova", is a synthetic...contains a nonfunctional truncated version of the kanamycin resistance gene (missing the first two codons)...pUCXKT will therefore have resistance to both kanamycin and ampicillin (from the pUC19 backbone). *False... allows the study of loci proximity and of the dynamic interactions between intra- and inter-chromosomal...LOC8R-PAGFP provides a novel strategy for assaying the dynamics of different mitochondrial subpopulations within... Ed Boyden’s lab. The enhanced sdChr construct, named CheRiff, exhibits high light sensitivity and fast...changes reduced system leakiness and increased the dynamic range, resulting in more user-friendly and tunable...

  3. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...broad dynamic range that typically spans 2–5 orders of magnitude, which includes a wide dynamic range ... may be light-sensitive. They also have a poor dynamic range, meaning that if your blot contains one protein...for multiplexing. They have a ten-fold greater dynamic range than chemiluminescence enzymes, making it...developer. Film is highly sensitive but has a limited dynamic range, which means it may take multiple exposures...background Chemiluminescence reporter Smaller dynamic range; no multiplexing Protein-based blocker...background Chemiluminescence reporter Smaller dynamic range; no multiplexing Wet transfer Increased...

  4. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...to red, or cyan to green). For example, mEosFP (named after the goddess of dawn in Greek mythology) switches...PA-FP is the naturally occurring Dronpa protein (named after dron, a ninja term for vanishing, and pa, ...trafficking, as well as organelle trafficking and dynamics (fission, fusion, etc). For example, by engineering... was able to visualize and quantify the fusion dynamics of mitochondria in healthy and apoptotic cells...at their fingertips the tools to probe cellular dynamics and function with extraordinary molecular detail...Mariusz, et al. "Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows...

  5. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...resistance gene, you’ll find the full name of the gene, the name of its gene product, and a description...cut the given sequence. The table includes enzyme names, the locations where the enzymes will cut (in basepairs...the plasmid sequence. The table includes feature names, locations, sizes, colors used to indicate them ... nucleotide sequence. The table includes primer names, sequences, binding site locations, lengths, and...

  6. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...for dTAG molecules and any questions to Behnam Nabet (behnam_nabet@dfci.harvard.edu), Nathanael Gray (nathanael_gray...This post was contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer ...et al.), and in vivo studies (Nabet et al.).   Behnam Nabet, PhD is a postdoctoral fellow at the Dana-Farber... the dTAG technology. Follow him on twitter @behnamnabet.   References Nabet et al. The dTAG system for...

  7. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... in your database to the effect of `dev_name` and `science_name`. You'll need to decide given the technical...things tracked. Terminology and language are tricky Names for things will trip everyone up at some point, ..., decide early on what to do in a namespace collision. It may even be worth it to have extra columns in... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...

  8. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...sequence or name. You can perform more in-depth analyses on each sample by clicking on the sample name or on...and provide basic information such as your sample names and guide sequences, and ICE will do the rest. There...shows a green checked circle in front of the sample name. If there was a minor error during processing, the...following parameters: Sample - The unique label name that you provided for each sample. Guide Target ...

  9. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...bright Recent series of enhanced monomeric NIR FPs named miRFPs (monomeric infrared FPs) (Matlashov et al...recommend using miRFPs and their enhanced versions named emiRFPs in your applications.  There is a whole ...distinct (e)miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their emission spectra....  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from cyanobacteriochrome... An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat Methods 11...

  10. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... to control the cytoskeleton and cell membrane dynamics. First, a quick refresher.  The tools below use...microtubule-severing protein, Katanin is of course named for the samurai sword called a katana. Opto-katanin...most-important component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, and more, often...proteins support vesicle trafficking and organelle dynamics, but with dozens of family members and many functions... the many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-...

  11. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...MicrobiGals created glow in the dark petri dish ornaments and microbe cookies! 🤗 🤗 🤗 We hope everyone... tree with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria...bacteria, fungi, viruses, and one microbe ornament even dyed with real microbial pigment. Check out the hashtag...hashtag to see the description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6 — Dr...

  12. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...have different names, and the software can only extract gRNAs for genes using the name that is used by...condensed text file containing a full and a short name for each gRNA, as well as the suggested gRNA sequence...one solution is to identify all the alternative names for your gene of interest (that were not found by...Green Listed), and test if any of the alternative names works better. To achieve this you could e.g. copy...

  13. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...Visualization of DA release dynamics during locomotion in freely moving mice Record DA dynamics of optogenetically... single neuron in vivo in Drosophila Detect DA dynamics in response to a threatening looming stimulus ...et al. "Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science...

  14. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...within an organism.  So how did plasmids get their name? In 1952, Joshua Lederberg set out to clarify the...and François Wollman and became the widely adopted name for these elements. At the time, the use of episome...resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102, with EcoRI and selected...store, QC, curate, and distribute them all in the name of making it a little bit easier for scientists ...

  15. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... intensity and wavelength can be used to study dynamic processes. Conversely, varied input patterns may... activity persisted in the inactive state. The dynamic range, defined as the difference between the lowest...They also reduced system leakiness and increased dynamic range to around 100-fold. These improvements will...system. Applications of these systems The large dynamic range of these systems ensures that they can be...

  16. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on to characterize the structural...CoChR), and Scherffelia dubia (SdChR). ShChR, nicknamed Chronos, is blue and green light-excitable and...than those of other channelrhodopsins. CnChR1, nicknamed Chrimson, is the first reported yellow-peaked ...0.4ms from 21.4 ± 1.1 ms); this new variant was named ChrimsonR. Plasmids containing Chronos, Chrimson... overexpression or knock-down, where signaling dynamics are influenced by the relatively long life-cycle...assembly of plant transformation constructs. As the name suggests, GreenGate is based on the Golden Gate ...

  17. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...handy and make sure your name tag is always visible. Some people learn names by seeing them. It is OK ...level. Right away you have something in common. Namely, the scientific question you are interested in ...OK to ask someone to see their name tag if you are one of those people. Meet people in sessions by sitting...

  18. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...probably know as TaqMan) and dsDNA dyes (commercial name: Sybr). However, there are other chemistries available...online tool that uses the  amplicon sequence and thermodynamic and experimental parameters (neighbor stacking...temperature range) to predict PCR melting curves and dynamic melting profiles (Dwight, 2011).  Reference Genes...prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web ...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...

  19. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...infection and to monitor their abundance and clonal dynamics over time using next generation sequencing (NGS...the ClonTracer library and monitored population dynamics after treatment with EGFR inhibitor, erlotinib...using the ClonTracer library to monitor clonal dynamics following drug treatment in cell line and primary... 1. Hyo-eun, C. Bhang, et al. "Studying clonal dynamics in response to cancer therapy using high-complexity...

  20. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...antibodies for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV viral...combined CRISPR / retrotransposon system with the pithy name "site-specific target-primed insertion through targeted...025-08877-4.  Two powerhouse tools combine for dynamic protein labeling By Alyssa Shepard To fully understand... the genomic targeting power of dCas9 with the dynamic labeling power of miniTurboID, a proximity ligation...
Showing: 21 - 40 of 336 results