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We narrowed to 196 results for: p

Showing: 21 - 40 of 196 results

  1. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...., Feliciano, P. R., Arif, A., Bohnuud, T., Fernandez, T. P., Gehrke, J. M., Grayson, P., Lee, K. D., ...updated by Jennifer Tsang in August 2020 and by Emily P. Bentley in February 2025. References and Resources...Grünewald, J., Zhou, R., Lareau, C. A., Garcia, S. P., Iyer, S., Miller, B. R., Langner, L. M., Hsu, J...., Levy, J. M., Tay, T., Newby, G. A., Maianti, J. P., Raguram, A., & Liu, D. R. (2018). Improving cytidine...McElroy, A. N., Pandey, S., Doman, J. L., Huang, T. P., Raguram, A., Banskota, S., Newby, G. A., Tolar, ...Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained genome targeting with near-...

  2. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...than the substance P peptide, but still quite small. However, unlike the substance P tag, they found that...involved a peptide from the neuropeptide substance P. This protein had a highly specific antibody to the... the tag could be quite small. However, substance P has an amide group added to its C-terminus post-translationally...

  3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan... https://doi.org/10.1038/nature14136 Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein...Zhang J, Tang C, Zhang X, Xu H, Shi L, Cheng L, Huang P, Yang H (2018) In vivo simultaneous transcriptional...

  4. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben M,...Merten O-W, Blouin V, François A, Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules..., Penaud-Budloo M, Broucque F, Blouin V, Moullier P, Ayuso E, Vandenberghe LH (2017) AAV-ID: A Rapid and...

  5. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...en.wikipedia.org/wiki/File:Crispr.png Barrangou R, Horvath P. Science. 315, 2007 Jan; 1709–1712 (2007). CRISPRdb..., Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu S, Mojica FJM, Wolf YI, Yakunin JvdO, Koonin... F. Science. 2013 Jan; 339 (6121): 819–23. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville...

  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller... C., Ingelsson, M., Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J. K., Breakefield, X. O., ...

  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...., Thomas, D., Stamnes, S., Holt, K., Sinn, P., May, A. P., & Paulk, N. K. (2020). Methods Matter: Standard... F., Le, T., Prado, J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., Sommer, J. M., Pierce, G. F., & Qu,... https://doi.org/10.1016/j.ymthe.2005.02.021 Zuk, P., Sanchez, C. E., Kostick, K., Torgerson, L., Muñoz...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ... a lentiviral vector. Naldini L, Blömer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. Science...Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau LM, Rebello T. J Gene Med. 2013 Feb...Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros F, Beuzard Y, Gluckman...Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467(7313):318-22. PubMed PMID...

  9. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...and other important information. Tip: A lowercase "p" is often used as the first letter of a plasmid name...and simply denotes that the object is a ‘plasmid’. “p” is for plasmid. pXXXXX-XXXX Step 1: Backbone name...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...which identifies a p value cut off. This method entails arranging all calculated p values from smallest...this by establishing a significance threshold at a p value less than or equal to the alpha value divided...the cut off for significance by finding the largest p value where its rank divided by the total number of...multiplied by the alpha value is less than or equal to the p value. There are drawbacks to these tests as they ...

  11. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... J, Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018...doi.org/10.1080/19420862.2018.1445456  Holzlöhner P, Hanack K (2017) Generation of Murine Monoclonal Antibodies...

  12. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...Fotouhi, M., Bolivar, S. G., Ruiz Moleon, V., Eckmann, P., Worrall, D., McDowell, I., Southern, K., Reintsch...Bandrowski, A., Virk, H., Edwards, A. M., McPherson, P., & Laflamme, C. (2023). Scaling of an antibody validation...10.12688/f1000research.154034.1 Laflamme, C., McKeever, P. M., Kumar, R., Schwartz, J., Kolahdouzan, M., Chen... A. M., Healy, L. M., Robertson, J., & McPherson, P. S. (2019). Implementation of an antibody characterization...McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., & Roach, K. M. (2021). TGFβ1 induces resistance ...Weston, C. A., Chachi, L., Roach, K. M., & Bradding, P. (2019). Validation of antibodies for the specific...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...recognizes a short, linear epitope derived from the P and V proteins of Simian virus 5 (SV5) (Randall et...doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski, P., & Randall, R. E. (1992). Construction of solid matrix-antibody-antigen...reporting of mechanical forces with PILATeS By Emily P. Bentley Time to get flexible! Alex Dunn’s lab and...plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn...

  14. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...10.1016/B978-0-12-801238-3.02523-X. Hofmann, C., Löser, P., Cichon, G., Arnold, W., Both, G. W., & Strauss, ...doi.org/10.1136/jcp.s3-6.1.39. PMID: 4376152. Rowe, W. P., Huebner, R. J., Gilmore, L. K., Parrott, R. H., ...R. L., Garcia, K. Y., Goodrich, J. S., Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan, C. Y., ...Rizzo, K., Bertumen, J. B., Morales, C., Oluniyi, P. E., Nguyen, J., … Chiu, C. Y. (2023). Adeno-associated...

  15. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...References Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner...from this paper at Addgene. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, Sasaki KE, ...Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (2018)...Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin...

  16. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...Y, Chik KK-H, Zhang X, Fung AY-F, Tsoi H-W, Cai J-P, Chan W-M, Ip JD, Chu AW-H, Zhou J, Lung DC, Kok K-H...Jia HP, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S (2006) Lethal Infection of K18...doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z,...

  17. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre! Roodolph (Roo) P. St Pierre is currently ... was contributed by guest bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre, founders of STEAMid...

  18. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert, D. (2020). Fluorescent ligands...78827-9 Breen, C. J., Raverdeau, M., & Voorheis, H. P. (2016). Development of a quantitative fluorescence-based...W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J., Ji, N., & Lavis, L. D. (2017). A general method...., Parisi, M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist...Grondin, Y., Rogers, R., Bousfield, D., & Demokritou, P. (2019). Development & characterization of fluorescently...

  19. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ....org/10.1177/002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding...In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue 6, p. e38313). Public Library of Science (PLoS). https:...

  20. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR, Maguire A, Rex TS...https://doi.org/10.1038/nbt1153 Odom GL, Gregorevic P, Allen JM, Chamberlain JS (2011) Gene Therapy of mdx...://doi.org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco ....201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, ...
Showing: 21 - 40 of 196 results