...Addgene blog? Or why we make certain videos? In this post, we invite you behind the scenes in our content ...uncommon to see requests for fact checks on a blog post or Tweet, followed by very detailed answers from...our incredible scientists. Expert reviews on blog posts are done on a case-by-case basis, as many of our...research and previously written Antibody 101 blog posts were used, as well as consults with microscopy and...designer not shown.   However, for our blog posts, we usually make figures in BioRender or use appropriately...gaps and add to them as techniques evolve. Blog posts get out of date as techniques and knowledge changes...blog topics or even reach out about writing a guest post (email [email protected].) We are here to support...

... Most proteins are then modified via an array of post-translational modifications including protein folding...synthesis of proteins from mRNA and the addition of post-translational modifications Learn more molecular... plans for downstream applications In this blog post we will summarize some of the more common expression...expression of mammalian proteins that require multiple post-translation modifications for proper protein function...promoters (SV40, CMV, and RSV) for robust expression post-transfection. Mammalian systems can express proteins...produce complex eukaryotic proteins with the correct post-translational modifications. There are two types...bacterial cells are not equipped to add appropriate post-translational modifications. In addition many proteins...

...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia... example). This blog post is about how to get the most out of your postdoctoral training experience. ... successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your postdoctoral experience,...your postdoc At the end of grad school, you searched for and eventually identified a postdoctoral mentor...the Howard Hughes Medical Institute. Today's postdoctoral fellows (PFs) face a number of challenges ranging...academia - the main reason most people enter postdoctoral training. Similarly, there are several factors...factors to consider when selecting a postdoc mentor and lab. These topics have been addressed in numerous...

...better communicated the science in my blog posts. This post highlights several of these free tools which...Researcher Toolbox blog post on social media for scientists Find science career blog posts Learn about other...usually the last thing I thought about when writing posts. Since then I’ve realized my figures are just as...options for drawing your own figure discussed in this post. It’s open source and available for desktop use ...

...What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest bloggers. Check... tools more in-depth (such as these recent blog posts on prime editing, IgnaviCas9, and Nanoblades), but...Check out this blog post for more information. Additional resources on the Addgene blog Download the...the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

...this blog post includes data until November 9, 2020. Between then and the posting of the blog post, we just...: Depositor features (plasmid deposits and blog posts showcasing the work of depositors) Wednesday: Why...

... facing students today is that many graduate and post-doctoral programs lack any formal or mandatory professional...employer’s career pages to look for current job postings. You may then reference the connection you made...fair when you formally apply for the position. This post on Business Insider nicely summarizes good job fair...monster.com, where you’ll find a variety of listings. Post your CV and make it visible, which will sometimes...well-groomed LinkedIn page. Here you can search for jobs, post your CV, and, most importantly, manage your professional...that you should follow, nicely summarized in this post on Forbes.com, and here on LinkedIn.com. Mentors...in your success. If you are a graduate student or post-doc in a traditional training program, and you hope...

...blog post about genome-wide screening using CRISPR Find all of our CRISPR pooled library blog posts Check...This post was contributed by the Open Repository of CRISPR Screens. Imagine you’ve just discovered that...guest bloggers from the BioGRIDS ORCS team. This post is a joint effort by the BioGRID ORCS team. For ...Check out all of our CRISPR blog posts Resources on Addgene.org Read our CRISPR guide Learn more about...

...other posts on scientific communication Find more tips for scientific networking Read this blog post for...This post was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps...standing at your poster – it’s a different experience to practice while seated or with your poster on your computer... to hold up your poster, and pack a few business cards to tack up next to your poster. Arrive early: You...before your poster session, or it may be held at an unfamiliar venue. Finding your poster board well ahead... your audience. Use your poster as an aid: Have you ever gone up to a poster where the presenter spends...time talking to the poster, as opposed to engaging with you? Be sure to use your poster as a visual aid, ...

...detecting SNPs. Learn more from this Addgene blog post Read the paper in Science Find the Cas14 plasmids...now possible. Learn more from this Addgene blog post Read the paper in Nature Methods Find the anti-CRISPR...be included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the...Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

...This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University....University. In this second post in our quick guide to working with Drosophila, you’ll learn how to maniupate expression...your favorite gene (YFG) in flies. Read the first post here. Once you’ve identified some fly stocks and...more details on making a fly line in the next blog post). For example, the hedgehog gene can be overexpressed...sometimes you’ll have to make your own. In my next post, I’ll explain the relatively straightforward process...publication references and web links from my first post and start designing your first fly experiment! ...

...feedback 15:38 - 17:06 : Post Grad Plans 17:07 - 18:29 : How did you hear about post grad opportunities and...them? 18:30 - 19:54 : How to think about picking a post-doc project 19:55 - 22:40 : Improvements that can...Improve the Research Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids...

...FOR Boston 2015 Blog Post Read Our Career Blog Posts Read Our Networking Blog Posts ...information about the postdoc work force, problems start with the question of “What is a Post-doc?” The National...biological research enterprise going forward, in this post I’ll point out some of the highlights and takeaways...during the symposium was “How many graduate students/post-docs?” You can find some of the more detailed questions...wanted to touch on in organizer David Riglar’s blog post. Essentially the goal was to determine what data... there is not particularly good data on how many post-docs there are in the U.S., their makeup (in terms...commonly thought of as postdocs. Unfortunately, even when universities have simple postdoc numbers sorted out...

...the Addgene blog Check out this blog post on navigating the poster session Find tips to make the most of...your conference attendance Read all of our blog posts about conferences ...crafting your own poster. What sets a scientific poster apart? First, a good poster should catch the ...this type of poster using Powerpoint. “Better Poster.” This new take on scientific poster design was conceived...conference, you need to first make your poster. While a poster generally contains the same sections as... how different posters can be. You have also probably thought about why certain posters resonate with ...tips to help you build a great poster.  Tips for designing your poster Check conference guidelines To...

...This post was contributed by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold...them overtime accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor...However when the ruling was issued, a joint blog post by Director of the NIH Francis Collins and Secretary...New Career in Science with Our Career Readiness Posts Learn Good Management for Scientists Resources ...get rid of postdocs; that current postdoctoral appointments may shorten in length; that postdoc salaries...slides here) there are many postdocs paid at much lower salaries). Postdocs (who are not in a primarily...exactly how postdoctoral salaries are changing. Transparent salary information for postdoctoral positions...

...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well...pack it in. I had just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge... other job descriptions for marketing positions posted on recruitment websites, this job specifically ...was a PhD student at University College Dublin & a post-doc a Cambridge University where he studied mechanisms...Macfhearraigh.    Read more posts about Scientific Career Development ...Studying for interviews is really important. For my Postdoc position, I tried to read at least 50 papers on... postdoc...

...1722491115. Addgene blog posts related to chromatin This is not the first Addgene blog post about chromatin--...very much 3-dimensional, as you can see by this post’s chromatin illustrations from Leah Bury of Microscopic...following lists of PIs, articles, genes, and blog posts are not comprehensive, so just because you do not...Addgene colleagues and guest bloggers have written posts describing new methods and tools for studying chromatin... have been considering some new techniques, the posts below may steer you in the right direction! CAPTURE-ing...are partially curated from the presentations and posters I saw at the 2018 Keystone Chromosome Architecture...

...time meeting with and talking to graduate students, post-docs, and PIs. I won’t pretend that that there aren...process of writing, editing, and publishing blog posts runs its course more quickly than writing scientific...warned me, the swift gratification of publishing posts can be addicting. Even more satisfying, because ... enjoyed: travel. I’m actually finishing up this post on a plane back to Boston from Kentucky where I ... PhD. Resources at Addgene Read Other Careers Posts Visit the Addgene Careers Page Learn about Management...

... Look at master's-level job postings  Look at master’s-level job postings on a job search website like...see yourself enjoying?  To find master’s-level postings, try adding “master’s” to your favorite search...technical writer,” among others. Don’t discount job postings looking for PhD applicants since many will also...decision, but hopefully the questions in this blog post can help you start to figure out if a master’s is...

...information that I decided to write a few blog posts. This first post will be based on recycling! With the popularity...Sign up for blog posts and updates from Labconscious. If you’re inspired by this post then I challenge...Find lab tips for your research Read another blog post about conservation in the lab Resources on Addgene.org...the box at any location where the United States Postal Service picks up mail. NEB actually started this...box, flip the flaps of the box so the pre-paid postage stamp is on the outside of the container, and drop...the box at any location where the United States Postal Service picks up mail. Recycle plastic packaging...