...how will it be sent to me? Description: This describes how the plasmid or other product (kit, pooled ...vector’s plasmid number here. Gene/insert: This describes what has been inserted into the backbone, and ...they’ll end up on the N or C terminus of the transcribed and translated insert. If it is an empty vector... sequencing. Growth in Bacteria: This section describes how to grow the bacterial strain in which the ...

...worked extensively with enChIP systems. Can you describe this technology and its advantages? Fujii: In ...enChIP system using CRISPR technology. Can you describe this technology and some of its potential applications... any other technologies that you would like to describe?  Fujii: I believe that "Biology is Method", i.e...Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts box. ...

...this technological gap, a trio of recent papers describe the development of CRISPR activation (CRISPRa)...packaging capacity than AAV. Such an approach was described recently by the Day Lab, who developed neuron-...High-throughput screening strategies Although the tools described above make CRISPRa more accessible to neuroscientists... in vitro and in vivo applications, the tools described above make CRISPRa in the brain relatively easy...

...experience and skills would help you do the job as described. Let’s imagine you’re applying to a job that ... your cover letter, you can expand on that by describing how you developed and used a ddPCR assay to answer...exactly what you would want to talk about! You could describe your organizational system, your rationale for...company/organization]. I am a [adjective that describes you] scientist with over [number] years of experience...

...that the term ‘antibiotic’ can also be used to describe anti-parasitic drugs and anti-fungal drugs, because...not finishing the whole course of antibiotics prescribed to them This may seem counter-intuitive considering...The take-home? Always take your medication as prescribed. How do we know which bacteria are resistant ...infection is still effectively treated. We can prescribe antibiotics for the shortest time period possible...

... Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment process used to evaluate oligonucleotides... of colors, so do aptamers! Corn was recently described in 2017 (Song et al., 2017). Corn naturally dimerizes...Corn under promoters for transcripts uniquely transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring... Red and orange fluorescent aptamers Corn was described in the same study as Red Broccoli and Orange Broccoli...

...update, we have added new sections to share and describe some incredible new CRISPR technologies developed...much-anticipated prime editing section and additions describing CRISPR’s ability to make large genome edits using...transposases or the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements...

...minimizing confounding secondary effects? An approach described as “knocksideways” (a British idiom for “taking...two-color optical excitation of neurons. The paper describes five previously unknown channelrhodopsins from...Nature 2012 article by Jinny Kim and colleagues describes their efforts to map the location and distribution...mGRASP plasmids. Additionally, the lab recently described the use of another set of mGRASP plasmids in their...after SLIC cloning. The general cloning strategy described in the associated publication can be used to generate...

... discusses some of the programs SITN runs and describes some of the more popular topics from the Science...telling. Chapter 5: The SITN Blog - Vini and Amy describe how the SITN blog is a source of scientific information...underwater? Chapter 8: Work-Life Balance - Vini describes how she balances working in the lab and working...

...These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5, ...former lab at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide variety...3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. ...

...CRISPR/Cas inspired genome editing was initially described by Cong et al. and Mali et al. in January 2013...Nine months later, more than 1500 publications describe work to improve the tool’s specificity, orthogonality...orthogonality, and multiplexibility in various species and describe an assortment of applications. Despite the ubiquity...

...transcription of downstream genes. Nihongaki et al describe the targeted activation of endogenous genes and...64543or 64542) When the reporter mRNA is first transcribed, it is bound by both the GFP and the RFP proteins...activation or repression. The TCS light sensors described in ACS Synthetic Biology (2014) represent the ...recent work in Nature Methods, Georgieva et al describe the rational production of a new series of epitope...several ways. The laboratory of Samie Jaffrey describes using these tags in an in-gel RNA visualization...articles every quarter so be sure to check in or subscribe to the blog to recieve the updates in our quarterly...

...retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed into mRNA before being...depending on the class of virus. RNA genomes can be transcribed into mRNA, directly translated into proteins,...

...RTs in this regard and is even able to reverse transcribe the extremely long, highly structured HCV genome...structured template!). MarathonRT also faithfully transcribes RNA to DNA with low error rates compared to other...from A. victoria the functionalized nanobodies described here can be used to target any protein fused with...

... of the major CRISPR lingo and will be able to describe the functions of the various CRISPR/Cas9 components...Bind to a guide RNA The guide RNA, which will be described in more detail below, enables Cas9 to cut a specific...arrays as found in the bacterial genomes are transcribed into pre-crRNAs containing both the spacer region...

...plasmids containing the indicated gene, and you can subscribe to these pages to learn when Addgene receives ... articles reporting chromatin-related research describe the production of plasmids that might be useful...colleagues and guest bloggers have written posts describing new methods and tools for studying chromatin ...

...New work from Konermann et al. and Yan et al. describes new Cas13d enzymes that average only 2.8 kb in...Like other Cas13 enzymes, the Cas13d orthologs described in these papers can independently process their... high targeting efficiency, both of which are described below. Yan et al.: WYL-domain-containing accessory...

...adoption of the technology. In 2012, Saito et al. described the first Nano-lantern, a fusion of a mutagenized...hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki et al. improve the brightness of previous...substrate furimazine. In addition to the previously described applications, the enhanced signal of eNL allows...

...27478040. The lung cancer mutant collection is described in this paper. Systematic Functional Interrogation...the genetic pathway reference collections are described in this paper Systematic morphological profiling...  The genetic pathway reference collection is described in this publication We also wrote an extensive...

...prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA... R-loops are particularly enriched in highly transcribed regions and repetitive regions, such as telomeres... and repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular...