...recombination to turn on or off the expression of shRNA. This system requires less work than using established...technical issues associated with high levels of shRNA expression. The capability to turn RUSH and CRUSH...the induction of both DsRed fluorescence and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre...

...sequence targeting the gene is cloned into a viral shRNA expression vector and the vector particles are delivered...

... GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular function, this ...

.... This system could be used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13...

... Choose from: Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “What does...

...and are especially abundant in neuronal synapses. shRNA silencing of one circular RNA they have identified...

...matching your search terms with gene and guide RNA / shRNA inserts Resistance Marker: This indicates the selectable...

...variety of applications including cDNA expression, shRNA-mediated knockdown, Tet and Cre-regulated expression...

...Although REPAIRv1 knockdown is more precise than shRNA knockdown, it still displays substantial off-target...

...paper, the authors used lentivirus to introduce an shRNA to downregulate expression of miR124a, a regulatory...

... were reported as up to 85%, broadly similar to shRNA and Cas13 systems in a range of mammalian cell lines...

...identified essential genes while previous Cas9 and shRNA library screens only identified  60% of the essential...

...substituting with knockdown approaches using siRNA or shRNA. If this alternative is not possible due to a lack...

...such as microRNAs (miRNAs), short hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger...

...lentiviral vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors...

...expressing GFP, tetracycline response elements, and shRNAs many of which are available at Addgene. Since GMAP...