...promoter is included in the pSico plasmid to drive shRNA expression. Other features that can be included ... lentiviral transfer plasmids normally used for shRNA expression? Yes, it is feasible, but first the promoter...within the transfer plasmid must be modified. Most shRNA‐expressing lentiviral plasmids, such as pLKO.1, ... to drive RNA pol III-directed transcription of shRNAs. cDNA expression requires RNA pol II, and thus ...

...libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains a unique...

...transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is flanked by...

...Polymerase III also transcribes small RNAs, such as shRNAs and gRNAs. Resources Addgene's blog , including...

...frequently accomplished through expression of an shRNA targeting the mRNA of the gene of interest. These...