...insert. pLKO.1 – scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid...Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B.1 Determine the optimal 21-mer... Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2...which The RNAi Consortium has built a library of shRNAs directed against 15,000 human and 15,000 mouse ...genes. Addgene is working with the TRC to make this shRNA cloning vector available to the scientific community...lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct...selection. Figure 1 : Map of pLKO.1 containing an shRNA insert. The original pLKO.1-TRC cloning vector has...

...MCS) Adding short tags - ex: HA or Flag Cloning shRNAs Background For the purposes of this tutorial we...can be used just as easily to add short tags or shRNAs to any vector (the procedure will simply differ...