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  1. Mammalian RNAi Tools

    Type
    Collection
    ...Kissler 12247 pLVTHM Expresses shRNA under the H1 promoter; shRNA or a H1-shRNA cassette from another vector...browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...for more information on shRNA design and delivery. Constitutive Lentiviral shRNA Lentiviral vectors for...constitutive shRNA expression ID Plasmid Description PI 10878 pLKO.1 ‐ TRC cloning vector shRNA expression...1864 pLKO.1 ‐ scrambled shRNA Negative control vector containing scrambled shRNA David Sabatini 11795 pLL3.7...Cre-regulated Lentiviral shRNA Lentiviral vectors for conditional (Cre-lox) shRNA expression ID Plasmid ...activating shRNA expression Tyler Jacks 11579 pSicoR Cre addition causes both EGFP and shRNA to be recombined...

  2. Lentivirus Plasmids

    Type
    Collection
    ...expression of shRNAs; addition of Cre turns on shRNA expression. See article for more cre-lox shRNA expression...the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. See plasmid ...10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning...cloning Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker. See plasmid 14749 for Thy1.1...21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid 21916 for Neo ...Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression ... shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression ...Retroviral shRNA expression pSico PGK GFP - Conditional shRNA expression through... different genetic element or want to express shRNA. Choose by: Species-specific expression Epitope ...markers Viral expression and packaging Reporters, shRNA expression, transgenics and genome modification...Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pLenti CMV Neo DEST - ...expression pMKO.1 puro - Retroviral shRNA expression pLenti CMV Puro DEST - Lentiviral...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...pAAV-Ptet-RFP-shR-rtTA AAV; shRNA cloning vector; for evaluation of shRNA efficacy using fluorescence;... lentiviral plasmid for inducible expression of shRNA; neomycin selection; plasmid 21915 has puromycin...vector for inducible expression of transgene or shRNA; see article for detailed information about cloning...Lentiviral vector for inducible expression of a miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see...On Fraser 44012 pInducer20 Lentiviral vector for shRNA expression; see article for additional tookit plasmids...

  5. Cre-lox system

    Type
    Collection
    ...Cre-dependent shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs... excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs,...Mammalian Sofroniew 25997 LV-Cre pLKO.1 Cre and shRNA coexpression CMV Lentiviral Fuchs 26646 pCAG-Cre-IRES2... expression and tetracyclin-dependent transgene/shRNA expression ApoE.HCR.hAAT Mammalian Ehmer 85578 pTC-ApoE-Tet... expression and tetracyclin-dependent transgene/shRNA expression ApoE.HCR.hAAT Mammalian Ehmer 85797 pSkipFlox...constructs, Cre expression promotes shRNA expression. Gene Switch: These constructs contain two genes of interest... Expression System PI 11578 pSico Cre activates shRNA expression, removal of EGFP; See also similar plasmids...

  6. Retrovirus Plasmids

    Type
    Collection
    ...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro ...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional...

  7. Plasmid Collections

    Type
    Collection
    ...all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set...

  8. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...tdTomato from the EF1 promoter pLL3.7-TNFa-shRNA3 72598 Expresses shRNA against mouse TNF-alpha mRNA from the...

  9. Plasmids for Stem Cell Research

    Type
    Collection
    ... of human Sox2, KLF4, L-Myc, Lin28, OCT3/4, and shRNA against p53 in different gene/insert combinations...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...leukoencephalopathy David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335...Josef Kittler 163858 VPS35 shRNA1 VPS35 Parkinson's Lei Lu 163859 VPS35 shRNA2 VPS35 Parkinson's Lei Lu ...
Showing: 1 - 10 of 10 results