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We narrowed to 10 results for: shRNA

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Showing: 1 - 10 of 10 results

  1. Mammalian RNAi Tools

    Type
    Collection
    ...browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more....transcribed, producing the shRNA. Dicer, an RNase III enzyme, binds the shRNA and cleaves it to produce...information on shRNA design and delivery, see resources below. Figure 1: Overview of shRNA-mediated RNA ...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids... Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources RNA interference (RNAi) is an... target sequence. The dsRNA can be delivered as shRNA (short hairpin RNA) or siRNA (short interfering ...via transfection or viral delivery of a plasmid. shRNAs are often delivered using viral vectors. Lentiviral...

  2. Lentivirus Plasmids

    Type
    Collection
    ...(Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See Ventura et al. ...the gene of interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — ...Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puromycin selection. See plasmid...10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid. Includes a stuffer for easy cloning...cloning. David Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker. See plasmid 14749 for...21915 Tet-pLKO-puro 3rd Inducible expression of shRNA with puromycin selection. See plasmid 21916 for ...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...basal activity shRNA/RNAi Gene silencing Browse RNAi empty vectors for cloning in shRNAs, including plasmids...Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP...a different genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope ...delivery, genome modification, reporter assays, shRNA expression, transgenics, and more. See the next ...retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones made by Addgene ...uses such as genome modification, reporter assays, shRNA expression, transgenics, and more. Element Details...Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pnCasPA-BEC - A cytidine deaminase-mediated...

  4. Cre-Lox and Other Site-Specific Recombinases

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    Collection
    ...deleted. shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs... excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs,...constructs, Cre expression promotes shRNA expression. Gene Switch: These constructs contain two genes of interest...

  5. Retrovirus Plasmids

    Type
    Collection
    ...Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — ...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression with GFP expression. See plasmid 8452...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA. Expresses rtTA. See Zuber et al. (2011) for additional...

  6. Tetracycline Inducible Expression

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    Collection
    ... pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for...plasmid for easier cloning and design of inducible shRNA expression, with puromycin selection. See Plasmid... al., 2011 ) for additional tookit plasmids for shRNA or gene expression. rtTA-Advanced H1-2O2 Stephen...vector for inducible expression of transgene and/or shRNA. See article ( Szulc et al., 2006 ) for Tet-Off ...Brugge Return to Top Additional Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids...

  7. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...tdTomato from the EF1 promoter pLL3.7-TNFa-shRNA3 72598 Expresses shRNA against mouse TNF-alpha mRNA from the...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...promoters, enhancers and untranslated regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing...

  9. Plasmids for Stem Cell Research

    Type
    Collection
    ... of human Sox2, KLF4, L-Myc, Lin28, OCT3/4, and shRNA against p53 in different gene/insert combinations...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335...pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1) NR4A2 GFP CMV Parkinson's Glenn Schafe 233274 pAAV-CMV-GFP-pA U6 shRNA (Rat ...ALS George Church 222870 pLKO.1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan 223563 pmCherry-Synaptojanin...Josef Kittler 163858 VPS35 shRNA1 VPS35 Parkinson's Lei Lu 163859 VPS35 shRNA2 VPS35 Parkinson's Lei Lu ...
Showing: 1 - 10 of 10 results