...ll prevent binding to any target protein that is still on the inside of the cell. However, assuming you...hidden epitopes. However, keep in mind that you will still contend with many of the same drawbacks to each ...early steps don’t even involve antibodies, they are still critical to the outcome of your IF experiment and...

...the bound protein fluorophores and quenchers are still relatively bulky. One of the advantages of oligonucleotides...stable and brighter version of the aptamer, but it still had limitations in vivo. Using a combination of ... a lower magnesium dependence. Though initially still unstable in eukaryotes, the Jaffrey lab has since...

...alter my intro to reduce the number of potentially hostile posters (this advice included leaving out the term...career path development--but the science trainees still have to lead on their own development. Please see...your own professional development etc. database. Still have questions? I am easy to reach and happy to ...

...2019) If you’ve taken the time to do this and are still struggling with idea generation, try this thought...to the same problem. If your lab/company/team is still working towards a culture of encouraging dissent...easier to work with than nothing at all - they’re still ideas, after all, and that is the goal of this exercise...

...the way we apprehend molecular complexes and is still a very useful tool.    Like a guitar hero (that ...SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and most used pairs to measure FRET... faster measurement systems, this technique has still many good days and good “gigs” ahead...No offense...

...circuit is sufficient to induce reinforcement (instills a sense of “reward”), then the animal will repeatedly...type of behavior, certain technical considerations still apply. Testing stimulation First, In lieu of electrophysiology...prove inhibition of neuronal activation, but it may still be possible to use c-fos expression to get the evidence...

...mutations, we still weren’t entirely happy with our editing outcomes and in many cases still didn’t get ... be screening a total of a few hundred clones - Still better than 10.000, right? (Kwart, Paquet, et al...

...the amount of research conducted on DMD, there is still no good treatment. The dystrophin gene is very large...approach. Since shorter forms of dystrophin can still be functional, exon skipping is a good option for...CRISPR therapy closer to the clinic, much work is still needed. First, CRISPR delivery must be optimized...

...track position 5 years after receiving their PhDs, still strive to become academic researchers themselves...universities to hire a glut of trainees at low wages will still be there without restructuring. Positive Points ...highlighting some of the important questions that still need to be answered about the makeup and welfare...

...was fascinated by CRISPR and rushed to test it. I still remember the day, March 16, 2014, that I got my ...transmission of the transgene, this technique is still in need of improvement. Also when working with CRISPR... discovered in an obscure bacterium and that we still have much to learn from biology in all its forms...

...themselves are not pathogenic, precautions should still be taken when using them. There are suspected cases... useful for a wide variety of experiments, care still needs to be taken when selecting which particular... other viruses, multiple freeze thaw cycles can still reduce AAV titer. For long term storage, make > ...

... can be used to aid design. These databases are still young and will require a collaborative effort from...arbitrary PAM definitions. These newer tools may still prove somewhat useful for the latest NTT targeting...protein, Cpf1. Separately, on-target efficacy is still a concept that only a few tools are trying to solve...

...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...of ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA...

...curriculum development team at eScience Labs. She still gets to write curriculum, but has taken on other...responsibilities.“I’d say maybe 25% of my day is still what I did as an SME, and then 75% of the time I...

...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other...

...and transformation. While restriction cloning is still routinely performed by many labs, a variety of other... of cloning has its advantages, most scientists still encounter several struggles with these techniques...

...analytical thinking, time management, information distillation, etc) are transferable to many different fields...thinking about preparing yourself for a career while still in graduate school, you’ll be well suited to follow...

... NpHR, can even be expressed simultaneously and still be individually activated with different lights ...immobilized in agar at the head with their tails still free, and different regions of the spinal cord were...

...Here are just 4 of the CRISPR-Cas9 FAQs. If you still have more questions after reading through these,...expression, though it is possible for the plasmid to still express without the G. Because the G is only one...

...cleaved off from the fusion protein while it is still bound to the glutathione resin, eliminating the ... proteins in mammals. Mammalian purification is still possible but requires optimization and often more...