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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 219 results for: ttl

Showing: 21 - 40 of 219 results

  1. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ... the Addgene lab. In this post, I’ll tell you a little bit about what I learned from a New England Biolabs...CosMCPrep system for this, but thought we could learn a little bit more about high throughput DNA prepping by ...

  2. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...reasoning,” the catchall term for a range of neat little tricks our minds perform to avoid cognitive dissonance...10.1073/pnas.1704882114 Kahan, D., Peters, E., Wittlin, M., Slovic, P., Ouellette, L. L., Braman, D., ...

  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed MitoTimer plasmids... can be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer reporter ... to orange) protein. Image courtesy of Roberta Gottlieb. Laker et al., J Biol Chem 2014 Apr 25;289...

  4. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ... Possible Adenovirus Remains Episomal (Little Risk of Insertional Mutagenesis) High Gene Expression...High Immunogenecity AAV Remains Episomal (Little Risk of Mutagenesis) Very Low Immunogenicity Infects...

  6. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...vector and Murine LoxP vector.   Turn it off for a little while Transient suppression of expression is faster... Lenti expression vector. Turn it on for just a little while Yes, there’s a CRISPR for that, too! CRISPR...

  7. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...social media, not from their institution), is how little agency they have in their salaries and benefits...suffice it to say, salaries are showing surprisingly little correlation with cost of living at the location...

  9. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...The PAM… that sneaky little bit of sequence that you hope is present next to the "perfect" guide sequence...make a precise amino acid change, there is very little flexibility in the location of the cut and subsequent...

  10. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...excited for this next step. But wait, now you feel a little panicked. You’ve never had an industry interview...day.  This whole process may seem foreign and a little overwhelming, but here is what I’ve found helpful...

  12. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ... in 1947 in Uganda, the Zika virus had received little attention and, for the most part, had been associated...databases to the production of validated sensors in as little as one week (Pardee et al, 2016). Using toehold...

  13. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...the protease cleavage sequence. The tag is then shuttled to the proteasome due to the presence of the degron...tagged protein. In this event, the degron domain shuttles the entire tagged protein to the proteasome for...

  15. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ..., creating a presentation for 8th graders was a little intimidating. Too simplistic and I would insult...section was arguably the most important, and it was a little worrisome for me since bioethics is an inherently...

  16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...feeling optimistic for the next tournament). Minus a little rain towards the end, it was another successful.... I’m aware that  “organizing the fun” sounds a little neurotic, but hey- sometimes fun needs to be had...

  17. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...accordingly in case you have questions or need a little assistance at some point during the procedure. ...with questions and concerns is invaluable. With a little thought you can build meaningful relationships ...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...access to reagents and equipment has become a bottleneck. In the last few years, scientists have utilized... work rapidly, providing reliable results in as little as an hour. Find the SHERLOCK plasmids at Addgene...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...Leaving a tight-knit team like that can and will be a little gut-wrenching, but you can get that feeling of ...editor, etc.--so everyone’s experience will be a little different. Some of these changes you may enjoy ...
Showing: 21 - 40 of 219 results