We narrowed to 740 results for: NSI

...resistant to antibacterial drugs. Resistance can be intrinsic, meaning a basic characteristic that naturally...resistant somehow overtime. One fantastic example of intrinsic resistance would be Mycoplasma species’ resistance...possess cell walls. As a result, Mycoplasma are intrinsically resistant to this class of antibiotic. Acquired...be unharmed by the compound it produces (think intrinsic resistance), but surrounding microbes would be...prescribed to them This may seem counter-intuitive considering the other three scenarios, but a short treatment...

...sure your cells have first reached an appropriate density. Cells in the exponential growth phase are alive... prevent the culture from reaching the desired density within a reasonable time-frame. For these cases... growth media, or if your plasmid is low-copy, consider reducing the antibiotic concentration. Growth ...accomplished by growing your culture to the desired density at 37°C and reducing the temperature or moving ...TB or 2XYT is optimal because of the high cell-density they support; however, minimal media supplemented...

...concentration of Taq polymerase, 2) increased PCR extension time, 3) increased concentration of MgCl2, 4) ...vector with Gibson assembly. While this is a comprehensive mutagenesis method, a drawback to using Gibson...scanning libraries. PFunkel: Of prior existing comprehensive mutagenesis methods, PFunkel offers the best...PCR-based approach for generating a customizable comprehensive mutagenesis library that’s ready to be tested...Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined Mutagenesis.” Ed. D. Dafydd Jones...

...antibodies, increasing the fluorescence signal intensity. However, you need to be careful when selecting...interest is not located on the cell membrane but inside the cell. Due to their chemical nature, antibodies...nucleus intact. If markers of interest are located inside the nucleus, a different fix/perm kit is required...that can enter cells freely are typically used. Inside the cell, the ester group is cleaved off by enzymes... yielding a stepwise decrease in fluorescence intensity in a (histogram) flow plot (Figure 4b). Through...

...metabolic engineering.  The E. coli genome has an extensive research history and characterization. Many proteins...cardiovascular disease, specifically stroke and hypertension (Iannaccone et al., 2009). Rodents have also...amphibians in science with early research providing insight into some of the most significant and fundamental... plant model organisms in the world. With its extensive scientific history, highly characterized genome...Genome sequence of the Brown Norway rat yields insights into mammalian evolution. Nature 428, 493–521 ...

...gene of interest) and prevent incorporation of extensive sequences used for cloning into the final translated...Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium... integrity, expression level, and aggregation propensity in four expression systems: E. coli, wheat germ...frequencies across timepoints or populations. This kit consists of a 48 plasmid adapter library, with each plasmid...Morgan’s blog post on the PUF Assembly Kit for some insights on its potential from its creators. We hope you...

...included inducible Cre, such as the tamoxifen-responsive CreERT, the coupling of Cre-lox with FLP-FRT ...limited due to low resolution or were very time-intensive, necessitating the development of a new technique...This system differs from Brainbow-1.0 in that transient Cre recombination is required to prevent constant...tissue was challenging due to low fluorescence intensity, caused partially by fluorophore photoinstability... the Brainbow technique will provide important insights into the complicated physical organization of ...

...prokaryotic minions that carried out the labor-intensive process of replicating your new plasmid: the Escherichia...certain restriction enzymes that are methylation sensitive. dnaJ Mutation in a chaperonin gene Increases...strains are all based on E. coli K-12 and are considered BSL-1. Table 2: Lab strains of E. coli Strain...Dam or Dcm methylated, allows for methylation sensitive restriction enzymes to cut the plasmid after preparation...tables are by no means exhaustive! For a more comprehensive list and additional information, visit OpenWetWare's...

...FRCaMP, FRCaMPi had better dynamic range and sensitivity, in part because its binding affinity is much... Zhu, Q., Eom, M., Fang, S., et al. (2025). A sensitive soma-localized red fluorescent calcium indicator...great feats of materials engineering as well as extensive validation of the cell and gene therapy, so please... this limitation by developing CRISPRkit, an inexpensive system for CRISPR experiments in classroom settings...Figure 6). This approach eliminates the need for expensive reagents and equipment like incubators, micropipettes...

...the Moloney Murine leukemia virus (MoMLV) and consisted of two plasmids: the packaging plasmid containing...vectors Although retroviruses are still in use, considerable efforts have been devoted to develop efficient...generation. The 1st generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector... from one batch to another. For experimental consistency it’s a good idea to purify and titer your virus...sucrose to the supernatant to create a gradient of density that favours virus precipitation. My tip for this...