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We narrowed to 885 results for: ANT;

Showing: 571 - 600 of 885 results

  1. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...identify what your plasmid contains and other important information. Tip: A lowercase "p" is often used...simple piece of information can often convey many important details. Once you know the backbone a plasmid ... on, you can usually derive: a) the bacterial antibiotic resistance, b) the promoter that drives the insert...position 295 was mutated to an Alanine, Q295A.  Mutant version: pBACKBONE-Flag-hGene(Q295A)-EGFP  Wildtype...

  2. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... do not express Cre or Flp. Here at Addgene we quantify the amount of transgene recombination for every...fibrillary acidic protein (GFAP) for astrocytes it is important to test it in the brain region of interest, especially...Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids... Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral... the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When Using AAVs Three Key Considerations...

  3. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...component of vaccines and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended... Genome Portal at the University of California, Santa Cruz, offers sequence data on five strains of Ebola...outbreak and earlier ones, along with links to the relevant scientific literature.  -In light of the current...Medicine have made their complete collection of relevant papers freely available here. -PLoS and The New...

  4. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...libraries also include 2,400 gRNAs without any significant hit across the E. coli genome as a negative control...PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE) base editors Keith Joung’... in human cells. Thus, they engineered two CBE variants with mutations in the rat APOBEC1 that decreases... fold and >3,800 fold in human cells with no significant reduction in editing efficiency. Read the paper...

  5. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    Published
    Nov. 29, 2018, 1:53 p.m.
    ...are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the...many others are still unknown. Identifying Cas14 variants In this quest to discover new and maybe more efficient...for a new genome-editing tool but it could be a fantastic add-on to a diagnostic toolkit, called DETECTR...

  6. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...typically grown in liquid culture without antibiotics to give the antibiotic resistance gene time to be expressed...being performed at Addgene, this adds up to a significant time savings. You can also save yourself time...homemade competent cells to ensure good efficiency.  Want even more tips for transforming your cells? Check...

  7. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...a request and order if approved.  I want to stay updated!  We want to keep you updated! Subscribe to the...will be available for request in three different quantities: 10x 100 µL (1 mL), 25x 100 µL (2.5 mL), or 50x...process. This process will take 1–2 days, and we anticipate approving the vast majority of requests.  If ...

  8. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...ability to label live cells make this system truly advantageous. This new technique, developed in Thoru Pederson... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and... live cell imaging of genomic loci. Ma et al. anticipate that the technique will be applicable to studies...shattering (chromothripsis). As this study joins the pantheon of useful CRISPR/Cas9 techniques, we at Addgene...

  9. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ... be able to store your sample without having substantial degradation and which experiments you can use...preventing the activity of DNase. While this is very important to preserve the stability of your DNA, it will...elution of plasmid DNA through their kit. The advantage of using a Tris buffer is that it allows better...that elution buffer onto your DNA. Knowing how you want to use the plasmid DNA can help you choose the best...

  10. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression...CRISPR is an incredibly fast-moving field, and we want to make it easy for you to keep up with new developments...editing plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3...

  11. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...is reflected in the biology, too. CRISPR is an elegant bacterial immune system and an efficient gene editing...sequence. The spacer sequence is the RNA guide, like a wanted poster that Cas9 uses to track down its suspect—the... repeat section of the crRNA, forming the repeat:antirepeat duplex. tracrRNAs also contain multiple stem...have a blog post on the structural biology of Cas9. Want a simpler guide to keep at your desk? We’ve got ...

  12. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...that had never been done at Addgene before. This meant I was responsible for establishing a new report ...certification on social media data, and I was given a fantastic spreadsheet template to build out a report on....more people who need it!  After a few months, we wanted to share these, and other, findings with the larger...to-dos, I also found time to go out to sushi restaurants for lunch with coworkers and visit the office...

  13. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ... effective tools but neglect some biologically relevant situations. Tissue-specific genes – the genes...its functions can be observed. Functionally redundant genes – multiple genes which function in overlapping...to generate a collection of monitored genes and mutants - viral integration, transposon, and many more!...10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020. DOI: 10.1105/tpc.12.7.1007...

  14. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...or personnel to constantly prepare them for distribution. One of the major advantages to depositing materials...depositor’s dedication to scientific sharing, we wanted to celebrate our depositing PI’s who have the most...Given the increasing popularity of CRISPR in the plant community, this has saved us hours of work responding...

  15. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic...one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the gene toxic...choice of ori depends on how many plasmid copies you want to maintain, which host or hosts you intend to use...amount of aeration, temperature, culture volume, antibiotic, and medium can all affect copy number. Some ...

  16. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ... drudgery to some scientists, MTAs are important—they guarantee certain rights and protections for depositor...another blog post: Why Do I Need an MTA Anyway?) Don't want to wait? Talk with your TTO Has a pending legal ...request MTAs and deposit agreements to basically instantaneous in many cases.  Suggest that they get an Addgene...

  17. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ... discipline and reduces the dissemination of unwarranted claims or unacceptable interpretations. A good...journal.  As a postdoctoral researcher studying plant development and abiotic stress tolerance, I have...peer-reviewer you wish for The last thing that I wanted to mention about the peer-review, is that as a ...ratio in Arabidopsis, (2) expression patterns in plants with enhanced sodium accumulation in their roots...

  18. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    Published
    Nov. 8, 2018, 3:47 p.m.
    ...Professor at the Lab for Innovation Science at Harvard, wanted to see how new tools drive innovation in synthetic...was moved exactly as is from one organism to a distantly related organism, the gene may not express well...showed a growing trend of sourcing genes from distantly related organisms. In 2006, less than 1% of the...it do? Those are the sets of questions one would want to have answered with a biosurveillance tool,” Kunjapur...

  19. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...minutes (incubating the full 30 minutes will not significantly improve the transformation efficiency).  5. ...primers; you need that free hydroxyl group! You may want to include extra extension time after the last cycle...mixing the PCR product with the TOPO vector, you may want to add extra salt to your reaction: Topoisomerase...next step before proceeding. Pre-warming your antibiotic-containing plate prior to plating your transformation...

  20. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...labs. We’re overjoyed to provide you with these fantastic educational resources and will be sure to keep...us at [email protected]. Thank you again for a fantastic year and happy reading! Top 10 blog posts of 2015...Addgenie Julian Taylor-Parker explains why you might want to use a specialized E. coli strain for protein ...Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and tired of having...

  21. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...University of Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter ...apparent in metabolic engineering. The authors elegantly demonstrated the potential of this to control ...metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory ON/OFF master switches...

  22. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...on the marketing or business background of the applicant. However, although this position did look for ... interviews Studying for interviews is really important. For my Postdoc position, I tried to read at least... current sales position interview was just as important. The problem was I knew I had a knowledge gap....teaches you about selling products which is an important part of the business and allows you to work with...

  23. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...enable? Boyden: There are many examples where you want to activate two sets of neurons and see how different...quarter of a million people have some kind of neural implant already. If we can use light to more specifically...many of them very popular. Why has it been so important to you to distribute these tools as freely as ... There are lots of neuroscientists out there; we want them to use these tools, so we can focus on the ...

  24. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...of a cell - cell free extracts Typically, if you want to make a protein – say green fluorescent protein...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and... hydrogels that they can touch. With BioBits, we want students to make the connection between the facts...Learn how the Arabidopsis research center brings plant science to life Resources on Addgene.org Browse...

  25. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...locked down, a South African company wanted to test if a type of antibodies from an Andean mammal called alpaca...diseases, so we were left with no choice but to quarantine in our houses, feeling helpless about the situation... more efficient, open, and collaborative. We now want to use this potential to make a difference not just...

  26. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...collaboration - a sense of dialogue and community among participants, clarification - new and useful information,...our all-company meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting...Meetings are for talking, not reading: Send around relevant documents, like proposals to review or project...discussions can happen “off-line” with just one or two relevant people. How often do you need to meet? Use trial...

  27. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...into my role, I realized that actually, I didn’t want to go to grad school. But, I didn’t know what else...great fit for my skillset and interests. As such, I want to be able to share more about this nontraditional...order to keep everything running smoothly, you’ll want to be really organized. Often, product managers ...behind a common vision. Use your network to your advantage  As the profession gains more traction in the...

  28. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...have that you really want, please let us know! And if you study chromatin and want to start sharing your...daily basis, the lists below provide a snapshot of relevant resources and a good jumping off point. The following...that histone genes frequently have many different variant names, not all of which can be listed here. If ...

  29. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...and indicate why other neuroscience newbies might want to get into the field. Find Optogenetics Plasmids...There were, of course, many companies selling antibodies, microscopes, and dissection tools, but there...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ...neuroscientist looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene...

  30. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...commonly a plasmid and can infer genetic advantages such as antibiotic resistance. Bacterial conjugation...primary mechanism by which bacteria have gained antibiotic resistance and virulence. Scientists have studied...integration site. The integrated phage remains dormant until it is triggered to enter the lytic cycle....
Showing: 571 - 600 of 885 results