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We narrowed to 834 results for: Eras

Showing: 571 - 600 of 834 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...biological tools, and with the right safety considerations, they aren't dangerous for controlled lab usage...and episomal expression will last ~2-7 days at moderate levels. With regard to target cells or organism...Transduce on! Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling...

  2. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...find some proteins of interest for which you have several antibodies in stock. Why are there so many options...let’s think about induced conformation changes. Several types of assays, such as ELISAs and Westerns, frequently...complex if you’re looking for proteins that are interacting with one another, or if you’re looking for a ...

  3. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...highlights from the past couple months including several pooled libraries: Cas13X and Cas13Y, two compact...It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied...the new viral preps from recent months: Second generation GRAB dopamine sensors from Yulong Li’s lab are...

  6. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...testing at Addgene meaning they come ready made to accelerate your research.   Why distribute virus? Plasmid...This service will further enable scientists and accelerate research." What viral preps are available...expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo- and...

  7. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...unfold, absorbing the stochasticity of molecular interactions and embedding individual diversity to get from...than what fits for applied research. I focus in general terms on a couple of very broad topic domains, ...networks, as much as with technical knowledge. I leverage a breadth of knowledge to put diverse aspects ...

  8. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...translate to a desire to do the research itself. Several years of exclusively writing lab reports and empirical...busy point of the first semester in which I had several projects nearing deadline (an animated video depicting...chance to carry out a comprehensive review of the literature informing the notion of “heading” in soccer as...

  9. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ... due to a few limiting factors.  To start, the average health science startup requires a great deal more...those ideas into commercial products and next generation companies.  We launched in the early spring of...(CFO).  As CEO, I am responsible for internal operations and building external strategic partnerships ...

  10. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...enter cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry...cells! After verifying the specificity of their luciferase assay, they concluded that components in the ...lethality and were sorted into single wells to generate clonal populations. After iTOP treatment, both...

  11. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...However, careers outside of the lab highlight “transferable” skills such as leadership, management, and ...impact your work? What design variables need consideration? Enrich your knowledge and skills outside of...tools such as the Individual Development Plan to generate a list of potential professions Google potential...

  12. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...in a cell or animal model. AAV plasmids contain several important elements, but among the most important...occurring.  How do I validate my ITR sequences?  Next-generation sequencing and Sanger sequencing might fail to...vector stability and sequencing results. Next-generation sequencing is also helpful in quantifying ITR...

  13. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... need a second GFP antibody?  Similarities:  Generated against full length GFP and converted to recombinant... also withstands denaturing conditions, high temperatures, and other chaotropic conditions better than...fluorescence in high molarity GdnHCl, at high temperatures, and in the presence of oxidizing agents. Adapted...

  14. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...reading, you can calculate the DNA concentration. Generally, A260 of 1.0 is equivalent to 50 ug/ml pure dsDNA...samples and are often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, .... This method is often used preceding next-generation sequencing or microarray studies and is not used...

  16. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...-rings are, in fact, permeable to CO2 at low temperatures (-80°C) and once in the tube, the CO2 can alter... storage, we then thawed the samples at room temperature and used the color of the liquid solution and...not all O-rings) were permeable to CO2 at low temperatures. We tested two different types of tubes: the...

  17. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...minor variation in experimental conditions will derail a research program (think a subtle difference in...to help your fellow researchers advance science overall. Communication is the issue Much of the problem...President. Her current responsibilities encompass overall leadership of the Cell Press editorial department...

  18. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ..., why it affects our emotions, and even what therapeutic properties it has. As with other works of Dr....of characters from five different families over several millennia as their paths cross with each other ...Nicholson Baker’s The Anthologist. The book ended up literally changing my life. Though I have an undergraduate...

  19. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...landmark Cell paper showing that it was possible to generate induced pluripotent stem cells (iPSCs) capable...has spent years studying how that one protein interacts with that one promoter can tell you, it can be...clinical trial patients with advanced macular degeneration; Laurenz Studer discussed his work with Parkinson's...

  20. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ... with a simple question: how do Cas9 and sgRNA interact with and dissociate from a DNA target? The surprising...et al., 2016. We next wondered if we could leverage this model (built from in vitro studies) to boost.... Figure 2: Single-stranded donor DNA interaction with the Cas9-DNA duplex. Image from Richardson...

  21. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...closely associated and function as one unit: Gβγ. In general, GPCRs show a propensity to associate with certain...receptor, which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed ...Chemogenetics uses genetically engineered receptors to interact with small molecules to elicit a response in a...

  22. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...talk at the Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and Beyond meeting earlier...III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed until the base editor...to an uninfected culture which results in less overall dilution and less stringent phage selection. ...

  23. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...out of these interactions, ask yourself how every piece of feedback you receive generalizes.  If someone...skillset rather than a permanent fix. Treat every interaction in your work as an opportunity to practice communicating...

  24. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ... how stretches of DNA on different chromosomes interact with each other. Chromatin regulation is complex...in the right direction! CAPTURE-ing Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation... An Improved Method for Studying Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR...

  25. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly There are a few important points... when choosing the vector you’d like to use to generate your new fly line: 1. Where would you like the...  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut. Cell...

  26. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... the “Analyze Sequence” button to pull up an interactive plasmid map powered by SnapGene and gain access...the static maps discussed above, but also have interactive functionalities. You can now hover over any feature...maps, the linear sequences hosted here are now interactive and have similar hover over features. As with...

  27. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...protein is based on the properties found in the literature or tabulated data that summarizes properties ...). However, the theoretical brightness ignores several important experimental conditions that are related...detector sensitivity) and the biological system (temperature, prokaryote versus eukaryote, background fluorescence...

  28. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...Transfection is the process by which foreign DNA is deliberately introduced into a eukaryotic cell through non-viral...diethylaminoehtyl (DEAE)-dextra neutralize or even impart an overall positive charge on DNA molecules so that it can.... "Chitin induces natural competence in Vibrio cholerae." Science 310.5755 (2005): 1824-1827. PubMed PMID...

  29. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...Plasmids 101 will provide you with an overview of general molecular biology knowledge and techniques. If ...with only white colonies is very suspicious! Refrigerate your plates: Placing plates at 4C for few hours...care in making your plates: X-gal is light and temperature sensitive and needs to be added to media after...

  30. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation that will permanently ablate...package cDNAs for genomic delivery and have many therapeutic advantages, such as tissue-specific targeting... to temporarily increase expression. There are several flavors of activators which can be used with this...
Showing: 571 - 600 of 834 results