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Showing: 571 - 600 of 758 results

  1. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...think of as an operating system for engineering an entire cell. Typically when people use plasmids, they ...much easier. So you could imagine engineering the entirety of a cell. That's a big long-term technical challenge...

  2. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...modeling isn’t up your alley, feel free to skip directly to the results. To start modeling our data, we...chemistry and chemical biology.       David R. Liu is Director of the Merkin Institute and Vice-Chair of the ...

  3. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...choose, you may not be able to elute the antibodies directly into your preferred buffer. If that’s the case...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

  4. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...impacted the experiments we wanted to perform. It required a great deal of time and effort to test and optimize...J. O., Outram, S., Koenig, B. A., Pereira, S., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives...

  5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...availability following cross-linking fixation; they require an additional step generally referred to as Antigen...retrieval here because it’s not quite as ubiquitously required for IF as fixing and permeabilizing.  References...

  6. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ... that if an essential gene plays a role in the desired screening phenotype, it may be missed because it's...transcriptional machinery to dCas9 and its RNA directed binding site. These methods include the fusion...

  7. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...the frontiers of fluorescence microscopy often requires more photons. For example, moving from transient...Unlike other systems, no genetically encoded tag is required as the small-molecule binding motif targets endogenous...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats...integrase-deficient lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector...

  9. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular...

  10. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...affinity purification, in 2009. However, iChIP requires insertion of recognition sequences of an exogenous...into the target region. However, it struck me that direct use of such engineered DNA-binding molecules even...

  11. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...about its limited resources. Rather than developing entire new teams in house to solve every problem (although...collaborators’ professional developments without directly contacting them. One great way to transition from...

  12. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...solution. Since this technique is intensive and requires an electron microscope, it is not routinely performed...a few ways:  Absolute quantification by qPCR requires generating a standard curve of known concentration...

  13. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...all of these platforms were developing, Google acquired Youtube and has been growing its online video ..., also known as the science videographer, is a retired botanist and oceanographer who has a blog and several...

  14. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...They enjoy the fact that each new challenge will require knowledge of new topics and that they will switch...analyses skills were applicable to my cases. I can directly use my understanding of the molecular behaviors...

  15. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...process of onboarding a new employee who's coming directly from grad school. The transition has been a bit...thought they would be working in a lab for their entire career. Yet even success in academia means you’...

  16. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...SaCas9, Zhang’s group used an approach called BLESS (direct in situ breaks labeling, enrichment on streptavidin...

  18. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...as Rep or other initiator proteins, they do not require host proteins for replication and can maintain ...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...

  19. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...someone who provides career advice and offers direction on your scientific project. Your scientific mentor...meaningful relationships in your new lab, learn the required techniques, and be ready to take on trainees one...

  20. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... with a distributor for that country. In China, direct shipments weren’t able to clear import control...., we launched the Addgene blog for topics that required a lot of nuance or detail. By the end of 2023,...

  21. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...course of infection. These binding events can be impaired by the electrostatic repulsion of the negatively...

  22. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...sequences, which often have absolute position requirements. For example, a KDEL ER retrieval sequence only...for subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University of North Carolina...

  24. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...., Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and optogenetics of Rho GTPases...Piatkevich, K.D. et al. A robotic multidimensional directed evolution approach applied to fluorescent voltage...

  25. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...electron microscopy (cryo-EM) protocols typically require high concentration and purity of the target molecule...particles (eVLPs) offers an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain no...

  26. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...forward scatter (FSC) and side scatter (SSC). To acquire these measurements, the cytometer illuminates the... “lymphocyte + single cell + live” population, acquired from the previous gating pipeline, about 53% of...

  27. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...to support your work. For example, if your work requires specialized equipment, try finding institutes ... institutes were able to provide the resources required for him to meet his goals. Therefore, he turned...

  29. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Similarly, placing UAS upstream of a transgene permits directed expression of that gene in cells that also express...systems, allowing for more complex control over desired gene expression. Take a look at all of the options...

  30. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...one since the formation of mEosFP’s chromophore requires a temperature below 30°C and this is not ideal...fluorescence localization data. However, this technique requires harsh fixation conditions to preserve cellular...
Showing: 571 - 600 of 758 results