We narrowed to 575 results for: BASE;
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TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....Listed - a CRISPR Screen Tool Green Listed is a web-based software tool freely accessible though http://greenlisted.cmm.ki.se...: PMC4486245. 4. Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-...
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Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....non-enveloped viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should be used...interested in using chemogenetic and optogenetic AAV-based tools to study neural control. Surprisingly, little... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....For example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural circuitry behind the...recent paper, de Jong and colleagues used rAAV FLEx-based vectors to answer this question, mapping and characterizing... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m....Transposable elements are split into two classes based on their mechanism of transposition: Class I TEs...Guide RNA-Assisted Targeting) system. INTEGRATE is based on a naturally occurring Tn7 transposon found in... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog PostPublishedMarch 26, 2019, 1:40 p.m.... on to learn more! Calcium sensor AAV The GFP-based calcium sensors are single-wavelength sensors with... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....write a few blog posts. This first post will be based on recycling! With the popularity of single-use ... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m...., etc). The cloning method is ultimately chosen based on the plasmid you want to clone into. Regardless... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....newest GRAB sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution required... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog PostPublishedOct. 18, 2017, 1 p.m.... creating the outline of a college level course based on their research interests. “So for me, I researched... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m....repair (HDR) are echoed in the amount of interest in base editors, which alter genomic DNA without the need... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m.... an AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids have two ITR sequences. The... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m....possible specific neurons responsible for the response based on the overlap between groups. They found that ... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m.... genetically-encoded voltage indicators (GEVIs) based on the ASAP sensors. The resultant JEDI-2P sensors... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....audience can understand the conflict. A science-based conflict gives you an easy framework with which ... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....lab and into the clinic as well. The first sdAb based therapeutic was approved in 2018, with many others... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....Kagiampaki et al., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m.... cellular phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171(2022).DOI... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m....Transformation CRISPR 101: CRISPR-Mediated Plant Base Editors... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m.... search term, i.e. “biology + masters.” For lab-based roles, look for “research associate,” “associate... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m....about six months, I learned there was a Genzyme-based running team called Running for Rare Disease that...