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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 611 results for: CaS

Showing: 381 - 400 of 611 results

  1. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...large (1-10) and small (11) fragments. In several cases an FP(1-10) fragment may be compatible with multiple...Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015...

  2. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...conjugated substrate is added to the wells. In the case where the detection antibody is biotin-conjugated... protein within a mixed sample. However, in this case your protein of interest is pre-incubated with a...

  4. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...the heading "Addgene Partial NGS Result." In some cases, there will be more information about our sequencing..., Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  5. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...the paternal for Prader-Willi syndrome). In both cases, the other copy of the chromosome is normally imprinted... exact genes, being improperly silenced in both cases. In both diseases, genes from both copies of chromosome...

  6. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ... providing feedback is needed for that. In many cases reports are not aware of issues until you bring ...what to do. Unfortunately this is not always the case or you may not feel comfortable reaching out to ...

  7. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...your intellectual freedom and integrity. (Not the case, as I’ve discovered.) As someone who has now seen...academia, as many of us know, this is not always the case. There are “difficult personalities” in every work...

  8. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...hits come from these known impurities, as was the case for clean sample AAV-44362. In addition to these...containing the suspected contaminating sequence. In most cases, the source of the contamination can be identified...

  10. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...potentially costly set-ups. However, there are many cases where the specific viral particles you need are ... viruses are too high for your budget. In those cases, you’ll need to produce your viruses in-house. ...

  11. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...can be faster and easier to work with. In either case, it can be useful to run a restriction digest of...   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  12. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...fluorophore is present in the sample (APC in the case of Figure 1). In Figure 1A, the population needs...shifted, consider your perpendicular lines. In this case, the population on the left is a horizontal line...

  13. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...that the emission wavelength can change. In a few cases, the initial state of the protein is non-fluorescent... yield of the protein, divided by 1000. In many cases the brightness is compared to that of EGFP which...

  14. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...the sample to allow for even expansion.  In some cases, labels are added after this stage.  Step 4: Expand...alleviated by maximizing label density. In some cases, nanobodies have been found to help improve label...

  15. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...responsible for each figure. Others still are more casual with a single presenter summarizing the paper’s...could be solicited via multiple avenues including casual request via email, in person, or instant messaging...

  16. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...activation sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless... system has been devised (8). References 1. Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J....

  17. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... adults alike enjoyed some friendly -or in some cases, serious-  competition with our corn toss boards...word of mouth - be sure to bring up your event in casual conversation to get people excited!    Laura ...

  18. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...other tools in conjunction with the ELN, in some cases the integrations provide a direct boost to reproducibility...restrictions based on permissions, however, in some cases it would be possible to grant those wanting to understand...

  19. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...relies on dimerization for activation, only in this case ligand binding would bring the relevant ends together...RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian cells...

  20. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...research being published on our own topic. Only on occasion do we then have time to read about research from...video abstracts in science journals: An overview and case study." Journal of Librarianship and Scholarly Communication...
Showing: 381 - 400 of 611 results