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We narrowed to 709 results for: LIC

Showing: 381 - 400 of 709 results

  1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...study its function is one of the most popular applications of fluorescent proteins. These fusion proteins...linker sequence short In the early days of GFP applications, many were concerned with steric hindrance by...between secondary structure (beta-sheets or alpha helices). An additional requirement is that the site of...

  2. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...strand. While this pathway may not be immediately applicable in DSB-induced genome engineering, it has biological...that can be harnessed by researchers for many applications. Your favorite Cas enzyme, a guide RNA to your...Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014)...

  3. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...comparison to a control sample. Southern blotting applications range from confirming the results of cloning...sequence thousands of reads at the same time. Other applications of sequencing, such as confirming the results...replaced these, these techniques still have useful applications and have informed many current techniques. For...

  4. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms...break your experiments!  First, closely read the publication, including the supplemental data. You’re most... both of which have extensive collections of publications and information on hardware, optogenetics tools...

  5. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...source of piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...technology that may be used for a variety of research applications, including making transgenic cell lines and ...

  6. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...vivo animal model should consider the possible elicited immune response from viral infection. Animal safety...rate limiting step and can immediately undergo replication and transcription. However, with scAAV the effective...only can we compare these methods in specific applications, we may also be able to use these comparisons...

  7. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...genetic code of living organisms and its societal implications even before the discovery of genes themselves... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...us to always consider the ethics and broader implications of our research.  Want the Genome Editing Trading...

  8. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...editing typically needs to be optimized for each application, and the efficacy varies widely — it still almost...editing variants Four years after their initial publication, the Liu lab returned to the reverse transcriptase...Image from Doman et al., 2023, under a CC BY 4.0 license. epegRNAs: Structured RNAs with improved ...

  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...that the antigen being detected in the intended application — here, flow cytometry — is the desired target...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi.org/10.7554...of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. eLife...

  10. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

  11. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...trick. Just flip, and press! Lab tip #2: Tube flick trick When combining reagents in the lab, it is ...the various reagents to a single tube and simply flick the tube several times. This will help ensure ensure...

  12. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...from Bruce Wetzel and Harry Schaefer, NCI, NIH | License   I’m updating it regularly as people send me suggestions...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...

  13. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...

  14. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...minipreps? If so, we’d love to have you write for us! Click Here to Contribute to the Addgene Blog Possible ...molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development...

  15. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

  16. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...in their own research. All posts from July 2016 Click on the links below to read all of our excellent ...

  18. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...will simply load an extra ladder or technical replicate (ie, leftover sample) to fill the relevant wells...using an antibody validated for your protein and application of interest or validating an antibody yourself...lysis Add sonication step to protein lysis Splice variants Check literature Posttranslational...

  20. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ... lend them many other functions for use in RNA splicing, imaging, and more. Abil says she is currently...says the new tool might even find therapeutic application in addition to their use in basic biology, for...
Showing: 381 - 400 of 709 results