We narrowed to 714 results for: Mak

...Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and what can they...excitation at 493 nm and max emission at 509 nm), making it useful in multicolor labeling particularly with...

...was awed by the teamwork and initiative it took to make all this happen with 80% of the team working remotely...Protection Program loan and received enough funds to make most of our payroll for the spring and early summer...

...whirlwind of skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative ...misinformation in the wake of a global pandemic can make for a sore reminder of how far we have yet to go...

...science communication research.   This summer, we’re making time to talk about my favorite thing - science ...but the foundation of all good communication is making sure everyone’s on the same page. So for our first...

...stop for refreshment along the way? What ecosystem makes your heart sing with delight? From developmental...other scientists, or science journalists, or policy makers. The term editor covers a wide variety of careers...

...extra extension time after the last cycle of PCR to make sure that the "A" gets added to all PCR products... the standard ligation reaction is fairly quick, make sure you stay organized and prepare everything you...

...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...

...from the Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward...that with this award from Addgene, we’ll be able to make big progress in elucidating association between ...

...?” and "What type of science careers would would make a good fit for me?" Whatever would I do next and...for sales and marketing jobs Like most choices we make, my job choices were mostly by chance. Luckily, ...

...tools out there to visualize genomic loci, what makes CRISPR-Sirius unique? Unlike fluorescent in situ...predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic loci...

...changes) the original identifier loses its meaning, making it difficult or impossible for researchers to track...verified and identifiable to save researchers time and make their work more reproducible. While Addgene plasmid...

...working in our laboratory! Don’t worry though, we make sure that everyone gets proper equipment and safety...engineer yeast to do similar things. The focus is to make yeast engineering accessible to everyone but these...

...Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-...restriction enzyme digests to DNA assembly. What makes it so forgiving, is the incorporation of a 3’-5’...

...published in the same journals as those that did make deposits to Addgene.  Insomuch as different journals... argument that Addgene is achieving its goal of making plasmid-based science more accessible…and accruing...

...Sequence- and ligation-independent cloning: A SLIC makeover In 2007, LIC received an important update, courtesy...for multi-fragment DNA assembly, and its low cost makes it ideal for researchers doing large amounts of ...

...control for a specific marker in your experiment. To make an FMO sample, you take a subset of your experimental... is prepared very similarly to a FMO sample: you make a sample from a combined subset of your experimental...

...terminal repeats (ITRs). ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV ...and disruptive mutations and truncations that can make vector propagation and sequencing a headache. How...

...then inserted into the host's genome and is used to make more virus. The most studied (and used in the lab...also transduce dividing and non-dividing cells, making it conducive to targeting all cell types. Altogether...

... Thus, the adjustable nature of viral libraries makes them the ideal vector for the job. Therapeutic ...immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...different constructs for the reporter to work and to make the desired genome edit:  A donor plasmid that ...