We narrowed to 716 results for: Met

...CASANOVA plasmids here! Bubeck F et al., Nature Methods 2018. PubMed PMID: 30377362. Studying neuronal...Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular Fluorescence Complementation (rBiFC...2in1 plasmid kits at Addgene! Mehlhorn DG et al, Methods Mol Biol, 2018. PubMed PMID: 29043675.Grefen C ...

...is useful for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression...or can interfere with the precision needed in metabolic engineering or other synthetic biology circuits...

...enzymes that you are using are not blocked by methylation like XbaI, ClaI, etc. If you are doing multiple...to be strategy specific, there are few general parameters that you can adjust: temperature and time of ...molar ratio of 1:2 (plasmid:insert), but this parameter has to be optimized according to the strategy ...

...pharmacological or genetic approaches, but only a few such methods exist and they only work for a subset of kinases...kinases. The Problem:  There is no common method for creating optically controlled kinases. The Solution:...KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating other signaling pathways...

...efficiency was similar to that of a comparable FISH method. Importantly, they did not observe labeling when...with both intronic and exonic gRNAs. In fact, the method is specific and sensitive enough to detect gene...genome editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed...

...been “working on new experimental and theoretical methods to push the scale of genetic engineering, with ...carry CRISPR circuitry, it can slow their growth. Something has to be done about that.  The second problem...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...

...coaching plans that are financially accessible. Sometimes only a few hours of coaching time can make an ...Coaching. One nice thing about a group is that sometimes this offers the option of matching with the best...a good match as a coach for many scientists.  Sometimes it can be helpful having a coach that can offer...

...controls, safety systems that are built into how something functions, were designed for this purpose. One...understand what safety precautions to take, and if something does happen, ensure you can receive appropriate... before you start an experiment, just in case something does happen.   Finally, the best way to be safe...

... this blog post that highlights five different methods to use CRISPR to detect SARS-CoV-2. Other protocols...Andrea Pauli and Julius Brennecke. They developed a method that uses loop-mediated isothermal amplification... after reverse transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes...

...minute! Fun fact! Electroporation is one popular method scientists use to promote transformation, but it...offer resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive ...inducible expression systems that allow tuning of metabolic pathways. Fertility (F) plasmids: microbial sharing...

...there are for off-target edits to occur. Although methods to switch on Cas activity using light or drugs ... Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical Development 9:390–401 . https://doi....natural brakes for CRISPR-Cas technologies. Nat Methods 17:471–479 . https://doi.org/10.1038/s41592-020...

... week): The AdEasyTM system is the most popular method for creating adenoviral vector constructs. It consists...required if rAdV is to be used in vivo. The standard method for purification of rAdV uses cesium chloride (...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

...combination of these methods provides unique opportunities to develop computational methods to better understand...UAS system for Caenorhabditis elegans." Nature methods 14.2 (2017): 145. PubMed PMID: 27992408. PubMed...

..., author names and affiliations, introduction, methods, results, conclusions, and acknowledgements sections...equally-sized columns, where the introduction, methods, conclusions, and acknowledgements sections are...The other poster sections like the introduction, methods, and figures flank the central region, and a scannable...

...relies on Gibson and other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken...which a precursor molecule is made, modified (sometimes many times), and isolated afterwards. To engineer...imagine, there is an array of distinct goals within metabolic engineering, synthetic product production, and...

...or poor cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem by optimizing...-relevant lines such as CAR-T cells. Plus, this method is fast! These editing frequencies can be achieved...fusion and membrane-targeting applications. Nature Methods, 1–9. https://doi.org/10.1038/s41592-023-02085-...

...concentrating a viral stock. Different titering methods can reveal differences in physical versus functional...the result of successful viral transduction. This method may not work if the virus does not have the proper... tropism to infect the packaging cells. Another method of ensuring viral production is to perform PCR ...

...after a few washes, binding is detected using a colorimetric reaction. After the ELISA step, the narrowed-down...remarkable 12 working days. While the lab used this method for COVID-19 research, this protocol holds promise...System for Characterization of Enzyme Variants. In: Methods in Molecular Biology. Humana Press, pp 133–148....

...decision a little easier if an industry postdoc is something that you’re considering. Like anything, there ...can’t afford to spend too much time working on something extremely risky or exploratory. Getting a job ...specific web sites for postdoc listings, so the method of posting varies between companies. Like most ...

...secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split...3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second  Edition), 2012...Culture Cloning Mammalian Cells with the Agarose Method Plasmids 101: Mammalian Vectors Resources on Addgene.org...