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We narrowed to 711 results for: Met

Showing: 381 - 400 of 711 results

  1. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...pharmacological or genetic approaches, but only a few such methods exist and they only work for a subset of kinases...kinases. The Problem:  There is no common method for creating optically controlled kinases. The Solution:...KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating other signaling pathways...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...efficiency was similar to that of a comparable FISH method. Importantly, they did not observe labeling when...with both intronic and exonic gRNAs. In fact, the method is specific and sensitive enough to detect gene...genome editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed...

  3. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...been “working on new experimental and theoretical methods to push the scale of genetic engineering, with ...carry CRISPR circuitry, it can slow their growth. Something has to be done about that.  The second problem...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...

  4. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...coaching plans that are financially accessible. Sometimes only a few hours of coaching time can make an ...Coaching. One nice thing about a group is that sometimes this offers the option of matching with the best...a good match as a coach for many scientists.  Sometimes it can be helpful having a coach that can offer...

  5. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ... this blog post that highlights five different methods to use CRISPR to detect SARS-CoV-2. Other protocols...Andrea Pauli and Julius Brennecke. They developed a method that uses loop-mediated isothermal amplification... after reverse transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes...

  6. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...there are for off-target edits to occur. Although methods to switch on Cas activity using light or drugs ... Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical Development 9:390–401 . https://doi....natural brakes for CRISPR-Cas technologies. Nat Methods 17:471–479 . https://doi.org/10.1038/s41592-020...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...or poor cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem by optimizing...-relevant lines such as CAR-T cells. Plus, this method is fast! These editing frequencies can be achieved...fusion and membrane-targeting applications. Nature Methods, 1–9. https://doi.org/10.1038/s41592-023-02085-...

  8. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...controls, safety systems that are built into how something functions, were designed for this purpose. One...understand what safety precautions to take, and if something does happen, ensure you can receive appropriate... before you start an experiment, just in case something does happen.   Finally, the best way to be safe...

  9. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...minute! Fun fact! Electroporation is one popular method scientists use to promote transformation, but it...offer resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive ...inducible expression systems that allow tuning of metabolic pathways. Fertility (F) plasmids: microbial sharing...

  10. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ... week): The AdEasyTM system is the most popular method for creating adenoviral vector constructs. It consists...required if rAdV is to be used in vivo. The standard method for purification of rAdV uses cesium chloride (...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

  11. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ..., author names and affiliations, introduction, methods, results, conclusions, and acknowledgements sections...equally-sized columns, where the introduction, methods, conclusions, and acknowledgements sections are...The other poster sections like the introduction, methods, and figures flank the central region, and a scannable...

  12. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...combination of these methods provides unique opportunities to develop computational methods to better understand...UAS system for Caenorhabditis elegans." Nature methods 14.2 (2017): 145. PubMed PMID: 27992408. PubMed...

  13. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...relies on Gibson and other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken...which a precursor molecule is made, modified (sometimes many times), and isolated afterwards. To engineer...imagine, there is an array of distinct goals within metabolic engineering, synthetic product production, and...

  14. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...concentrating a viral stock. Different titering methods can reveal differences in physical versus functional...the result of successful viral transduction. This method may not work if the virus does not have the proper... tropism to infect the packaging cells. Another method of ensuring viral production is to perform PCR ...

  15. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...after a few washes, binding is detected using a colorimetric reaction. After the ELISA step, the narrowed-down...remarkable 12 working days. While the lab used this method for COVID-19 research, this protocol holds promise...System for Characterization of Enzyme Variants. In: Methods in Molecular Biology. Humana Press, pp 133–148....

  16. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split...3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second  Edition), 2012...Culture Cloning Mammalian Cells with the Agarose Method Plasmids 101: Mammalian Vectors Resources on Addgene.org...

  17. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...many cases fluorescent proteins have usurped old methods or complemented them in ways that make them much... now become hallmarks of ideal FP timers: 1. Ratiometric determination of expression time The ratio of...FP-timer protein or protein fusion. This one protein method simplifies cloning and makes your experimental ...

  18. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...decision a little easier if an industry postdoc is something that you’re considering. Like anything, there ...can’t afford to spend too much time working on something extremely risky or exploratory. Getting a job ...specific web sites for postdoc listings, so the method of posting varies between companies. Like most ...

  19. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...laboratory populations with that sequence2. Both methods are easy to implement and have been tested in yeast5...can't be performed with two or more confinement methods. Since molecular, ecological, and reproductive .... M. & Bier, E. The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations...

  20. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...interest, scientists have instead developed novel methods to co-express your gene along with a reporter, ...the genes expressed from the same cassette is sometimes advantageous, particularly when only a portion...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...
Showing: 381 - 400 of 711 results