We narrowed to 453 results for: Spr

1. Learning Technical Skills at a Lab Central Workshop

   Type

   Blog Post

   Published

   Feb. 20, 2018, 2:30 p.m.

   ...Lab Management Software for Biology Learn about CRISPR Pooled Libraries Resources at Addgene.org Find...

2. Summer SciComm Series: A PhD in Science Communication

   Type

   Blog Post

   Published

   Aug. 23, 2022, 1:15 p.m.

   ...fabrics and mask designs could work to slow the spread of illness. I later helped run workshops where ...

3. Plasmids 101: Gram Negative and Positive Bacteria

   Type

   Blog Post

   Published

   Aug. 2, 2022, 1:15 p.m.

   .... & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland...

4. R Bodies: Membrane-Rupturing Microscopic Tools

   Type

   Blog Post

   Published

   April 14, 2016, 2:30 p.m.

   ...from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive...

5. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted...

6. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...more posts on the various protocols and helping to spread the word as new protocols come out! References...

7. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ... In mammalian cells this would be possible with CRISPR/Cas-based genome editing. Selecting a bright fluorescent...

8. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   Published

   May 19, 2020, 1:15 p.m.

   ... therapeutics, and other treatments to stem the spread of COVID-19. That’s why we’re proud to be working...

9. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ...temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic systems ...

10. Sweating the Small Stuff: Details in the Lab

    Type

    Blog Post

    Published

    Feb. 8, 2022, 2:15 p.m.

    .... If you’ve got messy handwriting, reach for spreadsheets, printouts, and even label makers to keep things...

11. A Novice's Perspective on Neuroscience - SfN2017

    Type

    Blog Post

    Published

    Dec. 15, 2017, 2:07 p.m.

    ...interactions with Alzheimer's, I found others using CRISPR to generate new knockout mice, using adenoviral...

12. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    Published

    June 29, 2017, 1:06 p.m.

    ...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display...

13. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ...efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system...

14. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...determine if a variant is causal for a given disease. CRISPR has made genomic SDM relatively straightforward...

15. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...engineering technology. Monoculture and chemical spraying, mainstays of modern agriculture, are now synonymous...

16. All in a Twist: dsRNA

    Type

    Blog Post

    Published

    Feb. 2, 2023, 2:15 p.m.

    ...021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA Interference ...

17. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...RNA. Keep a clean work area, which may include spraying your bench down with a product to get rid of RNases...

18. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ... (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https://doi.org...

19. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even...

20. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ...Blog Read the Plasmid 101 blog posts Check out CRISPR Meets Synthetic Biology: A Conversation with MIT...