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... vectors contain two origins of replication, one each for E. coli and Agrobacterium, along with a selectable...size, they shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication ...and codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency...

...representing the specific recombination site for each enzyme, were generated to test recombination efficiency...efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent RFP expression...determine the cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested...

...-infrared range, and that’s exactly what iRFPs achieved. Those proteins allowed a signal-to-background...conventional FPs. In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher...contrast agents for two-color imaging in animals. The approach relies on ultrasound waves as opposed to light...

...contributed to 9,000 publications in 2025 alone, each representing questions answered, hypotheses tested...using Addgene-shared tools to develop new vaccine approaches against emerging viral threats, or scientists...evolution of scientific tools and computational approaches, we ensured that access to reliable plasmid information...

... of affinity tags, the proteins they work best attached to, and the species they are compatible with are...nature of the size, structure, and other qualities of each tag class, they are generally best suited for their...101: Protein tags Antibodies 101: Epitope tags To Each HIS Own ...

...meetings, when Melina, our CSO and co-founder, approached me and asked about my current projects and personal...seeing early results that suggest our content is reaching more people who need it!  After a few months, ...followed with great discussion and questions. I achieved my goal of helping everyone understand the importance...

...proximity to an endogenous gene. Each trap uses a slightly different approach (described in detail below),...something species-specific) or a selectable marker. Each insertion line can then be isolated and screened...

...and Figshare but we are working on expanding our reach. We can’t save you the hard work of reading papers...ReFigure extension and visit ReFigure.org. You can reach the ReFigure team at [email protected] Please...Akin. She envisions a world where the impact of teaching and doing science is rapid, concrete and open ...

...protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI restriction sites with different...RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows the rapid generation...Epub 2009 May 3. PubMed. 3. Singh H, et al. A new approach to gene therapy using Sleeping Beauty to genetically...

...been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that carries....g. pMT-puro). Worm vectors There are several approaches to studying YGOI's function in worms. Some of...

...others in the field where to find it. A good way to reach others in the science blogosphere is to read and... great place to find opportunities for science outreach. Here in the North Carolina Research Triangle ...He got a little money for his trouble, too. 7)   Reach out to science writers in your area or at your institution...

...recycle our discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don...protocol for making your own Taq polymerase by Outreach Scientist, Andy Baltus. Addgene founder, Melina...illustration above) and bribed the guys in the hospital machine shop with coffee and donuts to cut the pieces of...

...Addgene has over 65,000 plasmids in the repository, each verified by sequencing, which makes the repository...really fast,” Thompson says. To do this, Thompson reached out to MIT labs in the Synthetic Biology Engineering... database. Through a series of simulations and machine learning, they created a classifier that predicts...

...unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine whether...recombinant antibodies via Addgene to maximize the reach and impact of your work. Please email [email protected] scientists to easily share plasmids with each other. Together, we can transform the antibody sharing...

...RecBCD in bacteria. When two repeating elements love each other very much undergo recombination, two smaller...plasmids and testing your prep can save you the headache of troubleshooting failed experiments. Pick the...References and resources References Al-Allaf FA, Tolmachov OE, Zambetti LP, Tchetchelnitski V, Mehmet H (...

...focus on the AAV transfer plasmid and take a look at each of its parts. Figure 1: Parts of an AAV transfer...AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids have two ITR sequences. The DNA...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M (2019) Adeno-Associated Virus...

...TA-based ligation and tagmentation preparation approaches described previously require a preliminary double... sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...believe that this simplified sequencing and analysis approach can be easily incorporated into standard QC regimens...

...of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization...located near the ligand binding site. A selective approach entails the use of the enzyme biotin ligase, which... bacteria refuse to grow. Eventual success was achieved by inserting BirA into a pMal plasmid (NEB) thus...

... need to dive as deep into your research at an outreach event as you might in a scientific paper. Build...you don’t like it yourself. Be proud of your achievements and accept and acknowledge your failures. Foster... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ...

...Latin for 'it'), as “a generic term for any extrachromsomal hereditary determinant”. His proposal, however...1960s when scientists began to study other extrachromosomal particles, particularly Resistance or R-factors...PMC1209583. Watanabe, Tsutomu, Chizuko Ogata, and Sachiko Sato. “EPISOME-MEDIATED TRANSFER OF DRUG RESISTANCE...