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We narrowed to 816 results for: ache

Showing: 381 - 400 of 816 results

  1. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization...located near the ligand binding site. A selective approach entails the use of the enzyme biotin ligase, which... bacteria refuse to grow. Eventual success was achieved by inserting BirA into a pMal plasmid (NEB) thus...

  2. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ... need to dive as deep into your research at an outreach event as you might in a scientific paper. Build...you don’t like it yourself. Be proud of your achievements and accept and acknowledge your failures. Foster... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ...

  3. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine whether...recombinant antibodies via Addgene to maximize the reach and impact of your work. Please email [email protected] scientists to easily share plasmids with each other. Together, we can transform the antibody sharing...

  4. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...RecBCD in bacteria. When two repeating elements love each other very much undergo recombination, two smaller...plasmids and testing your prep can save you the headache of troubleshooting failed experiments. Pick the...References and resources References Al-Allaf FA, Tolmachov OE, Zambetti LP, Tchetchelnitski V, Mehmet H (...

  5. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...Latin for 'it'), as “a generic term for any extrachromsomal hereditary determinant”. His proposal, however...1960s when scientists began to study other extrachromosomal particles, particularly Resistance or R-factors...PMC1209583. Watanabe, Tsutomu, Chizuko Ogata, and Sachiko Sato. “EPISOME-MEDIATED TRANSFER OF DRUG RESISTANCE...

  6. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...generation of various flux imbalances. The conventional approach has typically been to either systematically delete...networks and multiplex gene control. However, these approaches are often met with the challenges of regulatory... be layered with distinct programs controlled by each dCas9 regulatory switch. By no means limited to ...

  7. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ... but also how to fix it when things go awry. To reach that goal, his group develops tools to tinker with...within them work together or perhaps compete against each other. Getting two-color stimulation has been a ...time? Boyden: There are always considerations for each species. This could be a three or four hour conversation...

  8. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill...Assess your interest in a different field Outreach activities (e.g. Volunteer at a local high school...

  9. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...millions, if not billions, of dollars, but by approaching it from a new direction, we hope to do it much...Guo, Fengbo Zhou, Paddy O’Leary (UCSF). Our approach is to discover key human proteins that can be ...imagine, it’s also been quite the administrative headache receiving constant email requests and tracking...

  10. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... In 2012, Casey Maguire and colleagues from Massachusetts General Hospital and Harvard Medical School ...behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs have improved transduction profiles...in target cells and areas that are difficult to reach, and are more resistant to inactivation through ...

  12. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...bibliographic databases available, Web of Science. For each publication, Web of Science provides basic bibliographic... lends weight to the argument that Addgene is achieving its goal of making plasmid-based science more ...Special thanks to Addgenies Pamela McA'Nulty and Zachary Tribbett for helping compile the data used in this...

  13. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...microscopy) on the material pages associated with each item. There are multiple CRISPR tools available ...and David Root as ready-to-use lentiviral preps. Each library uses nearly 80,000 gRNAs to target around...infection and at least one screening-scale experiment achieving the gRNA representation recommended in the publications...

  14. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...Illumina sequencing and took a class at NEB’s new teaching space at Lab Central to learn all about it. LabCentral...looking to try out a new technique or reagent! Racheal Komuhendo is a Lab Technician at Addgene who is... Racheal Komuhendo...

  15. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...basically, instead of having to sign individual MTAs for each request, the depositing organization signs one form...years! This has helped us develop a fast, agile approach, which we’ve updated and refined as new technology...  Rachel Leeson...

  16. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...was very popular. People kept asking if we were teaching classes at the lab, so in December 2015 we held...as a nonprofit organization in the state of Massachusetts. Because we’re conveniently located next to ...wants to share his knowledge with the community by teaching a DIY plant engineering workshop. Community projects...

  17. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...NNGRR(N)  Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) 5' TTTV AsCpf1 RR variant...below). After years of research, we now know that each direct repeat, combined with its adjacent spacer...unique, foreign DNA target sequences specific to each individual crRNA. Bottom line, the “direct repeat...

  18. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...’s manual for the optimal working conditions for each enzyme. Watch out for methylation issues. Enzymes.... Bonus: knowing how much DNA you have loaded in each well will allow you to approximate the DNA mass ...however, you may need to run the gel for longer to achieve good separation of larger DNA fragments. I hope...

  19. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ... an off hands environment? Does the group expect each other to be physically present at certain times ...sure your advisors will be good role models and teach you to be the scientist you want to be. For much...Advice for Choosing a Research Project 9 Tips to Achieve Success in Academia  Advice for Scientists Starting...

  20. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ... personnel from the industry and brainstorm with each other. The event also featured a seminar on “Tools...scientific reproducibility” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the...research.  We’ve also involved ourselves in extensive outreach programs to encourage and embolden the spirit ...
Showing: 381 - 400 of 816 results