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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 609 results for: cas

Showing: 381 - 400 of 609 results

  1. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...breaches such as the Tuskegee syphilis experiment, the case of Henrietta Lacks, the Havasupai incident, and ...schools, community events, churches or mosques. On occasion, we also get interviewed for print media, radio...

  3. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... and serve as a type of positive control. In the case of an unfavorable donor-acceptor orientation limiting...molecules, detect cellular processes and signaling cascades, quantify mechanical tension (a molecular “spring...

  4. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...the paternal for Prader-Willi syndrome). In both cases, the other copy of the chromosome is normally imprinted... exact genes, being improperly silenced in both cases. In both diseases, genes from both copies of chromosome...

  5. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...potentially costly set-ups. However, there are many cases where the specific viral particles you need are ... viruses are too high for your budget. In those cases, you’ll need to produce your viruses in-house. ...

  6. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ... providing feedback is needed for that. In many cases reports are not aware of issues until you bring ...what to do. Unfortunately this is not always the case or you may not feel comfortable reaching out to ...

  7. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...hits come from these known impurities, as was the case for clean sample AAV-44362. In addition to these...containing the suspected contaminating sequence. In most cases, the source of the contamination can be identified...

  9. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...can be faster and easier to work with. In either case, it can be useful to run a restriction digest of...   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  10. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...fluorophore is present in the sample (APC in the case of Figure 1). In Figure 1A, the population needs...shifted, consider your perpendicular lines. In this case, the population on the left is a horizontal line...

  11. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...your intellectual freedom and integrity. (Not the case, as I’ve discovered.) As someone who has now seen...academia, as many of us know, this is not always the case. There are “difficult personalities” in every work...

  12. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...that the emission wavelength can change. In a few cases, the initial state of the protein is non-fluorescent... yield of the protein, divided by 1000. In many cases the brightness is compared to that of EGFP which...

  13. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...responsible for each figure. Others still are more casual with a single presenter summarizing the paper’s...could be solicited via multiple avenues including casual request via email, in person, or instant messaging...

  14. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...the sample to allow for even expansion.  In some cases, labels are added after this stage.  Step 4: Expand...alleviated by maximizing label density. In some cases, nanobodies have been found to help improve label...

  15. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...activation sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless... system has been devised (8). References 1. Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J....

  16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... adults alike enjoyed some friendly -or in some cases, serious-  competition with our corn toss boards...word of mouth - be sure to bring up your event in casual conversation to get people excited!    Laura ...

  17. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...copy number is high (del Solar et al., 1998).  In cases of compatible plasmids, different negative regulation...replication or partitioning machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually...

  18. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...the first time. They can then plan accordingly in case you have questions or need a little assistance at...questions later – maybe even the next day. In the latter case, you can say “I usually need time to gather my thoughts...

  19. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...Addgene relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert...are highly repetitive, such as those containing cassettes of the same gene multiple times.  Bioinformatics...

  20. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...other tools in conjunction with the ELN, in some cases the integrations provide a direct boost to reproducibility...restrictions based on permissions, however, in some cases it would be possible to grant those wanting to understand...
Showing: 381 - 400 of 609 results