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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 699 results for: eed

Showing: 381 - 400 of 699 results

  1. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ... suggest our content is reaching more people who need it!  After a few months, we wanted to share these...of support and independence that allowed me to succeed. As a team, we talked through slide design, discussion...

  2. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...into this workflow? CRISPR both speeds up the process and can help weed out a lot of poor drugs before ...

  4. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... often used interchangeably with the term sgRNA. Seed sequence: The 8-10 bases on the 3’ end of an RNA...CRISPR Cheat Sheet  Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...

  5. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...cloning and expression from plasmids, due to only needing one open reading frame Ability to access unique...to producing single-domain antibodies without the need for a species that naturally produces heavy-chain...

  7. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...advisors they choose will be ones that help them succeed after the training period. While there are many...is important in both cases. As a PhD student you need to learn how to be a scientist. Your PI should be...

  9. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ..., making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the packaging...adenoviral helper plasmid which contains the proteins needed for the virus to replicate,  and the transfer plasmid...

  10. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...isotope conversion, gives users the flexibility needed for multiplexing. In the case above, you can’t ...this will not affect single-plexed experiments, it needs to be addressed when multiplexing. It is imperative...

  11. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...many microns in response to pH changes without the need for ATP or other chemical fuel sources.” In her ... how you can customize it for your experimental needs. Note: Casilio is no longer available through Addgene...

  12. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...should be stable in the cell. gDNA guides will also need to be transfected into the cells of interest. In...Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it seems that ...

  13. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...DNA Cloning: A Personal View after 40 Years.”Proceedings of the National Academy of Sciences of the United...Biologically Functional Bacterial Plasmids In Vitro.” Proceedings of the National Academy of Sciences of the United...

  14. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...colleagues and what I was researching, but I decided I needed a new challenge. Looking for sales and marketing...opportunities to expand their skill sets?", “Have they succeeded in what they have done?” and “Would their academic...

  15. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...YOUR PLASMID BUILD YOUR PLASMID is a game where you need to apply basic molecular biology, cell biology, ...and one “Transfer Plasmid” game board. Once they agreed on each element to use, they had to place the selected...

  16. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...misfolding and recombination during virus production needed to deliver CRISPR-Sirius into cells. They named...labeling of chromosomal loci in human cells.” Proceedings of the National Academy of Sciences of the United...

  18. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...can tailor our communications to better meet the needs of the people we are talking to. Emma Harcourt ...polarized beliefs on controversial science topics. Proceedings of the National Academy of Sciences of the United...

  19. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...another option for targeting GFP. But why might you need a second GFP antibody?  Similarities:  Generated...Stroik Microscopy techniques have been moving at the speed of light over the last decade, but the fluorescent...

  20. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...beautiful graphs! I like to look at these graphs when I need that extra push to stick with my writing routine.... The phrases in Phrasebank are generic, so they need to be edited to suit your particular field of research...
Showing: 381 - 400 of 699 results