We narrowed to 682 results for: eng
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TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....staining can be a challenge in the best of cases. But it can be particularly challenging when your only ...
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Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....Because mNeonGreen wavelengths lie between the green and yellow fluorescent protein wavelengths, it can be imaged... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog PostPublishedJan. 24, 2019, 2:24 p.m....23817127. PubMed Central PMCID: PMC3713494. Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal...reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke, Meng-Tsen, and Takeshi Imai. "Optical clearing and index... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....Regardless of excitation wavelength, Keima emits at 620 nm. The ratio of signal strength excited at 586 nm to... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....screening platform, Oliver Griesbeck’s lab set out to engineer a new FP. Their aim - to red-shift the boundaries...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....spring requiring an overnight hike in. Navigating challenges: collecting and processing environmental samples...,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable between 31-100... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m....other media outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs...the Advancement of Science. She is enjoys broad engagement through science communication with a variety ... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m....solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering While the vast majority of CRISPR plasmids in...validation process for CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....from the MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on ...the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog PostPublishedJuly 21, 2020, 1:15 p.m....different wavelengths of light to different degrees and many molecules have a specific wavelength that they... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....distance. From front to back: David Gordon, Jeff Guo, Fengbo Zhou, Paddy O’Leary (UCSF). Our approach is ...to Margaret Soucheray, Jeff Guo, Paddy O’Leary, Fengbo Zhou, Helene Foussard, and Dave Gordon) have been... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence, cell... by directed evolution. They then optimized the length of both linkers to generate a reporter that would... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....lines from becoming the new HeLa cells?), genome engineering and stem cells, and the difficulty of sharing...into a pig. In stark contrast to recent genome engineering technologies, stem cell techniques are neither... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m....starting cell culture work. 3. NEB Tools by New England BioLabs In my lab we do a lot of genetic construction...experiments. Not all of our lab enzymes are made by New England BioLabs, but the NEB Tools app comes in handy when... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m.... Complementary to the released strand, 127bp in length, With 36bp on the PAM-distal side of the break ...al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery.... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m....comments section below! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Resources... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m....scientists and technical people) is linked to engagement and access to growth opportunities, not salary...zone when you lose track of time because you are engrossed in a task. Things seem to fall into place frequently... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....expression of R-mAbs is that it can easily be engineered. Like humans, mice express multiple subclasses... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....specific behaviors. Chemogenetics uses genetically engineered receptors to interact with small molecules to...Designer Drugs (DREADDs) (Roth, 2016). DREADDs were engineered from members of the human muscarinic receptor...