We narrowed to 533 results for: erf

1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ... in gene therapy research to reduce promoter interference and/or drive high expression of a transgene ...

2. Communicating Your Science With Help From ComSciCon

   Type

   Blog Post

   Published

   July 19, 2018, 1:12 p.m.

   ...a committee that your research proposal is the perfect fit to their institutional focus and that you're...

3. Improved Plasmid Maps Powered by SnapGene

   Type

   Blog Post

   Published

   June 29, 2017, 1:06 p.m.

   ...cloning experiment early on. Beyond these simple but powerful display improvements, when you click on any plasmid...

4. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   Published

   May 25, 2017, 2:30 p.m.

   ...experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns and eNL for many...

5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ...publications), it will be possible for many others to perform additional functional assays for these mutations...

6. Addgene’s Viral Service - Why Virus? Why Now?

   Type

   Blog Post

   Published

   March 23, 2017, 2:30 p.m.

   ...The viral vector project team develops methods, performs extensive quality control, generates  educational...

7. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...How can I tell if my enzyme will cut? Whether performing a digest for cloning purposes or for diagnostics...

8. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ... Molecular Biology Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research...

9. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ...quite small—20 amino acids—so we don’t think it interferes with the function of the protein. Myers: We’ve...

10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...treatment with a BRAF inhibitor. (The test was the perfect complement for one conducted earlier by the Zhang...

11. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ...the time spent by the scientists designing and performing the experiments. This cost varies hugely based...

12. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ...days. There’s also a CRISPR for that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 ...

13. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...Antibody-based assays are complex and antibodies may perform differently in different samples and experimental...

14. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...immunogen. A different type of purification can be performed to isolate antibodies only recognizing your protein...

15. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ... to avoid contamination, take extra care when performing RNA extraction. RNA is inherently not as stable...

16. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...CreLite-mediated recombination was found in both deep and superficial tissues of zebrafish studied, such as the brain...

17. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...state of the photoswitchable proteins offers a powerful approach to control protein-protein interactions...

18. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    Published

    Jan. 14, 2019, 2:08 p.m.

    ... At Addgene we will never ever stop planning, perfecting, and learning, but there are a lot of things ...

19. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ...will use Addgene’s RESOLUTE plasmid collection to perform a functional screen in mammalian cells to identify...

20. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ...primer to 40-60 bp. It is always a good idea to perform primer optimization, especially if you are having...