We narrowed to 411 results for: genome

1. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ...simply amplify a gene of interest from the human genome, for instance, it may not express at all in E. ...

2. To Codon Optimize or Not: That is the Question

   Type

   Blog Post

   Published

   Nov. 12, 2020, 2:15 p.m.

   ... that they can effectively inhibit translation. Genome Research 23:604–615 . https://doi.org/10.1101/gr...

3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...protein homomerization in Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org/10.1101/gr.231860.117...

4. Bioinformatics at Addgene

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   Blog Post

   Published

   July 14, 2022, 1:15 p.m.

   ...had created a shared resource of pipelines for genomic data. Thanks to the flexibility inherent in Nextflow...

5. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ...(Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015)  Reference Feng...

6. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...stable integration of resistance genes in their genomes. If you need a plasmid that can go between plants...

7. Hot Plasmids: Winter 2025

   Type

   Blog Post

   Published

   March 11, 2025, 1:15 p.m.

   ...generated offspring tomato plants that inherited the genomic edit but not the editing machinery. Together, these...

8. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ... the delivery vector can be integrated into the genome to introduce the genetic modification(s) contained...

9. Antibiotic Resistance: An Old Solution but a New Problem

   Type

   Blog Post

   Published

   Nov. 10, 2016, 3:30 p.m.

   ...Resistance Genes Are Used in the Lab Probe Phage Genomes for Host Binding Proteins Learn How to Use Our ...

10. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...prey fusions can either be generated using random genomic fragments from the organism of interest, or a defined...

11. Plasmids 101: Control Plasmids

    Type

    Blog Post

    Published

    April 9, 2015, 3:29 p.m.

    ...cells. In experiments employing gene targeting or genome editing technologies, such as RNAi or CRISPR, non-targeting...

12. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...RNA, where it promotes translation of the viral genome in eukaryotic cells.1,2 Since then, a variety of...

13. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    Published

    Aug. 5, 2014, 6:51 p.m.

    .... In some cases, typically consortia involving genomic screens, the data stream is so prolific that raw...

14. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    Published

    June 20, 2024, 1:15 p.m.

    ...a mix of positive and negative regulators. The genome of E. coli is about 4.6 million base pairs long...

15. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...All About Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional...

16. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ...interpreting results.  The recent revolution in genome editing methods (most notably CRISPR/Cas mediated...

17. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...is licensed to New England Biolabs by Synthetic Genomics, Inc. ...

18. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...chemicals to precipitate out the high molecular weight genomic DNA; filter the remaining plasmid DNA through a...

19. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...

20. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    Published

    May 18, 2018, 7:32 p.m.

    ...example is our series of blog posts on the defunct genome editing technology NgAgo). In addition, there are...