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...thanks for depositing your plasmids with Addgene. Howarth: We submit all our plasmids that may well be of use...encoded way. SpyTag is nice and small, just 13 amino acids, but SpyCatcher is a protein. You can fuse SpyCatcher...

...Addgene.org Find plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent...acoustic reporters for your research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau...

...tools (and can have secondary functions as detection aids.) When a single protein is expressed, with the intent...antibodies, resins, beads, and other purification aids readily available. Choosing the best tag for your...Antibody protocols  Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags...

...steps into this field, we’ve curated microbiology plasmids from the repository onto one handy Microbiology...these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright and early on Saturday...Microbiology Resource Page Deposit Your Microbiology Plasmids with Addgene ...

... in the field, and enabling access to multiple plasmids used for various platform applications. For more... out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or ...467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L,...

...generate covalent bonds with positively-charged amino acids. Unfortunately, the location of biotin incorporation...Resources on the Addgene Blog Check out other Hot Plasmids Find Additional Posts on Mol Bio Techniques Find... Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies...

...transduce? So why use a virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...

...urgent requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits...support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating essential...-related slowdown. More scientists are sharing plasmids and other reagents every day, and this crisis ...

...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...

...protein guide Find plasmids for subcellular localization Find fluorescent protein plasmids ...translation in U2OS cells. HA is small, only 9 amino acids, thus it is translated quickly by the ribosome. ...

...been coming to the Addgene website in search of plasmids. Now, scientists are beginning to see Addgene ...Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing...Search for a plasmid Find Addgene's most popular plasmids ...

....115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other strategies...10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable and...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

...fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson Fluorescent Protein...

...whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally at 260 nm. Proteins...spectrophotometric methods that measure all nucleic acids). These methods are more sensitive than UV absorbance...Addgene.org Visit the Molecular Biology Reference Find plasmids for your research Browse protocols for molecular...

...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...

...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that... Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library  ...

...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR..., give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor...

...rough approximations of the total number of CRISPR plasmids for that particular organism available at Addgene...their fast adoption. However, in many cases the plasmids themselves are only the starting point for the...that you can use along with lentiviral packaging plasmids to create your own lentivirus stocks. Our research...

... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...

...how they’ll make it easier for you to find the plasmids you need. We'll be monitoring and improving the...this blog post for tips on using BLAST to verify plasmids. Analyzer guide A quick guide to all of ...browse the repository and quickly identify the plasmids and features you need for your next experiment...