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We narrowed to 678 results for: ids

Showing: 381 - 400 of 678 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...transduce? So why use a virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...

  2. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...generate covalent bonds with positively-charged amino acids. Unfortunately, the location of biotin incorporation...Resources on the Addgene Blog Check out other Hot Plasmids Find Additional Posts on Mol Bio Techniques Find... Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies...

  3. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...

  5. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...urgent requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits...support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating essential...-related slowdown. More scientists are sharing plasmids and other reagents every day, and this crisis ...

  7. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ....115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other strategies...10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable and...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

  8. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...rough approximations of the total number of CRISPR plasmids for that particular organism available at Addgene...their fast adoption. However, in many cases the plasmids themselves are only the starting point for the...that you can use along with lentiviral packaging plasmids to create your own lentivirus stocks. Our research...

  9. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...gene of interest in a cell or animal model. AAV plasmids contain several important elements, but among ...containing the AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019... 101: Pseudotyping Viral Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide...

  10. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...

  11. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that... Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library  ...

  13. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally at 260 nm. Proteins...spectrophotometric methods that measure all nucleic acids). These methods are more sensitive than UV absorbance...Addgene.org Visit the Molecular Biology Reference Find plasmids for your research Browse protocols for molecular...

  14. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR..., give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...

  16. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...how they’ll make it easier for you to find the plasmids you need. We'll be monitoring and improving the...this blog post for tips on using BLAST to verify plasmids. Analyzer guide A quick guide to all of ...browse the repository and quickly identify the plasmids and features you need for your next experiment...

  17. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website,...mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists ...Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

  18. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins at the beginning.... Now, we've turned some of the most requested plasmids into lentiviral preps to speed up COVID-19 research... has now expanded to offer many of our control plasmids in new serotypes, as well as a new Cre dependent...

  19. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...mutagenesis to generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...

  20. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...antibodies, such as inclusion of non-canonical amino acids or peptide tags. 5) Strong protein-protein interactions...ConjugatesAntibodies 101: The Basics of Western BlottingPlasmids 101: Biotinylation Resources on Addgene.org...Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene...
Showing: 381 - 400 of 678 results