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  2. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...with repeat elements, such as viral plasmids with inverted terminal repeats. We use NEB Stable to transform...

  3. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...

  9. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...

  11. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...blastn or blastp, due to the additional searches involved in translating the nucleotide sequence into all...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...easily accessible organ that can be monitored non-invasively over time. Importantly, it is an immune privileged...

  19. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ...
Showing: 381 - 400 of 455 results