We narrowed to 465 results for: invs
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  TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m.... actually infect cells. These assays typically involve infecting a target cell line with your virus and...
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Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function... - 
  
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....and it may also be aligned to plasmids in our inventory containing the suspected contaminating sequence... - 
  
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....two 48-well plates of clones) which enables investigators to identify specific clones with desirable properties... - 
  
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....essentially are messed up chromosomes with multiple inversions. They are unable to participate in genetic recombination... - 
  
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m.... methods add an additional viral passage. This involves taking your first round of virus, called P1 (or... - 
  
Hot Plasmids: Summer 2025
TypeBlog PostPublishedAug. 28, 2025, 1:15 p.m....titration. Can't wait? Browse our In-Stock AAV inventory or use our Packaged on Request service for thousands... - 
  
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....probably best to avoid AID tags. If your project also involves imaging at some stage, then choosing a HaloTag... - 
  
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic... - 
  
Transferable Skills Guide: Managing a Team
TypeBlog PostPublishedJan. 30, 2018, 2:42 p.m.... so plan accordingly - but think of it as an investment: once the person is trained you will have more... - 
  
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....and of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However... - 
  
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",... - 
  
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m.... yeast (109-1010 members). This selection step involves using a commercial kit for in-vitro translation... - 
  
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....design and analyze batches of CRISPR samples. We invite you to jump in and try it out, or take a look at... - 
  
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....with repeat elements, such as viral plasmids with inverted terminal repeats. We use NEB Stable to transform... - 
  
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The... - 
  
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....characterized in terms of CRISPR use, you will want to invest some time in optimizing and testing the efficiency... - 
  
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....that will provide a similar resource for those involved in monocot engineering projects. The MoClo tools... - 
  
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....blastn or blastp, due to the additional searches involved in translating the nucleotide sequence into all... - 
  
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m.... that even though these early steps don’t even involve antibodies, they are still critical to the outcome...