We narrowed to 462 results for: invs
-
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....probably best to avoid AID tags. If your project also involves imaging at some stage, then choosing a HaloTag...
-
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....and it may also be aligned to plasmids in our inventory containing the suspected contaminating sequence... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....two 48-well plates of clones) which enables investigators to identify specific clones with desirable properties... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....essentially are messed up chromosomes with multiple inversions. They are unable to participate in genetic recombination... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m.... yeast (109-1010 members). This selection step involves using a commercial kit for in-vitro translation... -
Transferable Skills Guide: Managing a Team
TypeBlog PostPublishedJan. 30, 2018, 2:42 p.m.... so plan accordingly - but think of it as an investment: once the person is trained you will have more... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....design and analyze batches of CRISPR samples. We invite you to jump in and try it out, or take a look at... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....and of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....with repeat elements, such as viral plasmids with inverted terminal repeats. We use NEB Stable to transform... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....characterized in terms of CRISPR use, you will want to invest some time in optimizing and testing the efficiency... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....that will provide a similar resource for those involved in monocot engineering projects. The MoClo tools... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....blastn or blastp, due to the additional searches involved in translating the nucleotide sequence into all... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....easily accessible organ that can be monitored non-invasively over time. Importantly, it is an immune privileged... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....interactive. If you can think of a good analogy that involves student participation, make it happen! How should...