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  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers developed viral...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...

  2. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...colony PCR primers The first and perhaps most important step to colony PCR is designing primers. There...for cloning. This type of control is especially important for insert-specific primers. If your negative ...

  3. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...the type II Cas9-based system, require a single-component nuclease to mediate cleavage rather than the multi-subunit...After sequencing and searching for cellular RNAs important for Cpf1 function, they found that mature crRNAs...

  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...solvent. This tightly-packed structure explains the importance of the entire GFP protein, which is almost completely...chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts the wavelength...

  5. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ... care, but they need to know that you have an important life too. They will respect you if you show respect...You can handle that, right? Big tip: this is an important topic to discuss and agree on before you get enmeshed...

  6. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...implemented, impacted the entire company. It is important to note that the person who submitted the idea...making something the best it can be that it seems impossible to start at all. This is why perfectionists tend...

  7. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...select for your vector. Some antibiotics block important cellular pathways shared by bacteria and eukaryotes...Finally, in some cases a researcher may choose not impose selection on cells and instead to use a vector ...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...virus’s most abundant proteins and a critical component for viral genome packaging. Addgene recommends...including in human retinal organoids and live mice. Importantly, REVeRT avoids the introduction of extraneous...

  10. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...toxin-antitoxin roles. Restriction modification systems are composed of restriction endonucleases and methyltransferases...described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin that...

  11. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...fluorescent proteins (smFPs). smFPs are literally composed of FPs fused to multiple epitope tags. Indeed,...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... passive mode, the stronger is the depressive component of the animal behavior. With the availability ...of viral vectors to fine tune gene expression temporally and spatially, exciting times are ahead for behavioral...

  14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... example all C’s will edit to T’s. Thus, it’s important that there are no “bystander bases” if you choose... particular gene isn’t feasible. For example, temporary repression or knockdown allow you to study essential...

  15. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ... in a particular organism. Why is codon usage important? Translation and protein synthesis relies on transfer...(Mauro and Chappell 2014).  Ultimately, it is important to take a close look at your experiment and gene...

  16. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Collection aka ATCC where I work. It is virtually impossible to work in cell culture, microbiology, or virology...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

  17. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...fusion based on your experimental needs. Equally important, before cloning you must consider whether and ...subcellular environments, including the organelles that compose the secretory pathway, endocytic vesicles, mitochondria...

  18. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... theory, is an easy technique, but it is very important to follow some simple rules to avoid common pitfalls...purpose of your studies. These parameters are less important when designing a biosensor, as the distance between...

  19. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...should be supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in...Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat...

  20. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...generations of scFvs against two antigens that are impossible to generate scFvs against in vivo: antigens found...intensity of a tagged protein. SunTag has two components: 1) a protein of interest that’s tagged with ...
Showing: 381 - 400 of 650 results