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We narrowed to 668 results for: mpo

Showing: 381 - 400 of 668 results
  1. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... increasingly politicized. It would be nearly impossible for a career scientist such as Zimmer to introduce...self-promotion, and personality is playing an increasingly important role in science. The science produced by the quieter...
  2. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...toward saying that host-virus interactions are an important part of the biology of adenoviruses (AdVs). AdVs... Although host-virus interactions likely seem important for any virus, we will see that they are very ...
  3. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Because it is made at the same time as the other components of the vector particle, some of the RT protein...In both cases, the lipid membrane is the only component that is not dependent on viral genes. It is derived...
  4. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...will link biomolecules to the gel and add gel components to polymerize. Step 3: digest the sample to allow...isotropic expansion depends on how well the gel components penetrate the sample, how well the gel polymerizes...
  5. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...interaction with other proteins.   It is important to note that research activities in a CURE must...opportunity to make new discoveries. Provost notes the importance of acknowledging student apprehensions about ...
  6. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...higher HR frequencies than quiescent cells. It is important to know that HR is often mutated in cancer cells...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious...
  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...experiments didn’t provide any information on other important factors such as what is the best promoter to use...successfully replicate published works. Of particular importance to these efforts is the open sharing of negative...
  8. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...gene-expression sequences, and more. By combining these components, one can generate constructs to visualize cell...facilitate the construction of plasmids with many components. The creators of the MXS-chaining method have...
  9. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers developed viral...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...
  10. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...colony PCR primers The first and perhaps most important step to colony PCR is designing primers. There...for cloning. This type of control is especially important for insert-specific primers. If your negative ...
  11. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...the type II Cas9-based system, require a single-component nuclease to mediate cleavage rather than the multi-subunit...After sequencing and searching for cellular RNAs important for Cpf1 function, they found that mature crRNAs...
  12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...solvent. This tightly-packed structure explains the importance of the entire GFP protein, which is almost completely...chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts the wavelength...
  13. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ... care, but they need to know that you have an important life too. They will respect you if you show respect...You can handle that, right? Big tip: this is an important topic to discuss and agree on before you get enmeshed...
  14. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...depositor-provided sequences to confirm the correct plasmid components. For more information on this process, see our...After a run on our Illumina MiSeq, the data is imported into our assembly pipeline that runs with Seqera...
  15. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ... perfect Our first tip, and arguably the most important, is to take some time to sit down, plan your cloning... plates of frozen glycerol stocks. It is very important that these stay frozen at -80 °C, since multiple...
  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...toxin-antitoxin roles. Restriction modification systems are composed of restriction endonucleases and methyltransferases...described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin that...
  17. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...fluorescent proteins (smFPs). smFPs are literally composed of FPs fused to multiple epitope tags. Indeed,...
  18. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...select for your vector. Some antibiotics block important cellular pathways shared by bacteria and eukaryotes...Finally, in some cases a researcher may choose not impose selection on cells and instead to use a vector ...
Showing: 381 - 400 of 668 results