We narrowed to 662 results for: mpo

... step, you’ll want to ensure that your buffer components are compatible with your conjugate and conjugation...immunohistochemistry, and immunocytochemistry. It’s important to note that some antibodies are better suited...

...conditions when compared to FRET. However, it’s important to note that BiFC is irreversible — FP fragments...interact or not? — FRET is preferable when the temporal dynamics of PPIs are the focus of study. Many...

... an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13 enzyme fused to an RNA adenosine... Abudayyeh, Omar O., et al. "C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector...

... increasingly politicized. It would be nearly impossible for a career scientist such as Zimmer to introduce...self-promotion, and personality is playing an increasingly important role in science. The science produced by the quieter...

...experiments didn’t provide any information on other important factors such as what is the best promoter to use...successfully replicate published works. Of particular importance to these efforts is the open sharing of negative...

...that can be monitored non-invasively over time. Importantly, it is an immune privileged compartment, meaning...underway and reported results are encouraging. Importantly, a significant amount of basic research is being...

...skills. Why are transferable skills so important? It is important to identify which transferable skills...

...gene-expression sequences, and more. By combining these components, one can generate constructs to visualize cell...facilitate the construction of plasmids with many components. The creators of the MXS-chaining method have...

...lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers developed viral...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...

...colony PCR primers The first and perhaps most important step to colony PCR is designing primers. There...for cloning. This type of control is especially important for insert-specific primers. If your negative ...

...the type II Cas9-based system, require a single-component nuclease to mediate cleavage rather than the multi-subunit...After sequencing and searching for cellular RNAs important for Cpf1 function, they found that mature crRNAs...

...solvent. This tightly-packed structure explains the importance of the entire GFP protein, which is almost completely...chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts the wavelength...

... care, but they need to know that you have an important life too. They will respect you if you show respect...You can handle that, right? Big tip: this is an important topic to discuss and agree on before you get enmeshed...

...virus’s most abundant proteins and a critical component for viral genome packaging. Addgene recommends...including in human retinal organoids and live mice. Importantly, REVeRT avoids the introduction of extraneous...

... passive mode, the stronger is the depressive component of the animal behavior. With the availability ...of viral vectors to fine tune gene expression temporally and spatially, exciting times are ahead for behavioral...

...recent lineage tracing approaches that led to important insights in developmental biology. Microscopy-...PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2 and application...

... in a particular organism. Why is codon usage important? Translation and protein synthesis relies on transfer...(Mauro and Chappell 2014).  Ultimately, it is important to take a close look at your experiment and gene...

...implemented, impacted the entire company. It is important to note that the person who submitted the idea...making something the best it can be that it seems impossible to start at all. This is why perfectionists tend...

...timetable for the Singletons in their bins.  The next component of Binning Singletons is when we bring all the...never went away. While he has frequent bouts with Impostor Syndrome, Joe has found his people through meetings...

...these reagents in both plant and animal systems. Importantly, they allow transient delivery, enabling transgene-free...annealing of the two oligos. Figure 1: Components of TnpB, schematic of pKb-TnpB1 and pKb-TnpB2...