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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: rel

Showing: 381 - 400 of 628 results

  1. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...particularly useful for understanding a specific effect related to an experimental manipulation. Common inbred ...Mice are fed or injected with tamoxifen, which relieves natural inhibition of the Esr1 domain that blocks...

  2. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...socioeconomic or educational background, cultural or religious affiliation, and ethnic or personal identity. ...symposia, or courses in social, health or policy-related topics, as time permits. Also seek out opportunities...

  4. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate. Eastman et...human glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library at ...

  5. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...expand already-existing sets. Note that this tool relies on a stochastic search algorithm rather than pre-designed....8b00333 Pryor, J. M., Potapov, V., Bilotti, K., Pokhrel, N., & Lohman, G. J. S. (2022). Rapid 40 kb Genome...

  6. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ... choose primary antibodies from more distantly related species and/or use narrowly specific secondary ... antibodies raised in different, and distantly related, species, and secondary antibodies raised in the...

  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... from Ashley et al., 2021.   The AID system relies on a transport inhibitor response 1 (TIR1) expressed...induce DSBs within minutes. New CRISPR plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab...

  8. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...restriction enzyme digest and compare it to some reliable controls. 2. Viral stocks may be sensitive to ...reducing the culture volume into which the virus is released, a more concentrated virus solution can be obtained...

  10. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...research goals in focus. Developing these skills relies upon biologists and computer scientists learning...neuron knockouts so that we can perturb factors related to worm motion. Working on the Cat Microbiome Project...

  11. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...are much better at remembering these things on a relaxed phone call than when first confronted on a stage... are for presentations, not discussions. I will rarely work with a panel if slides are allowed. Don't ...

  12. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...their academic science careers in order to head to Ireland and take a chance as entrepreneurs. To make things...that the learning curve is pretty steep. But we can rely on one another to celebrate good news or share a...

  14. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...Fusing EGFP to the self-labeling FlavinTag made a reliable one-component magnetoresponsive system that could...Chemical amplification of magnetic field effects relevant to avian magnetoreception. Nature Chemistry, 8...

  15. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...serotype, as some rAAV serotypes are abundantly released into the culture media, while others remain cell-associated...Wilson, J. M. (2010). Efficient serotype-dependent release of functional vector into the culture medium during...

  16. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...individually count 500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency... anywhere from 10 ng to 1 μg of DNA. It often correlates with the size of the library, but generally needs...

  17. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...heartbeat – a muscle contraction – is directly related to the electrical impulse of the cardiac cells....and this voltage is particularly physiologically relevant in neurons, myocytes, and endocrine cells. It ...

  19. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... require careful selection or design to ensure reliable readout and avoid biases. The lab of Alejo Rodriguez-Fraticelli...cell membrane while AF, fused to a payload, is released into the synapse. Once AF binds to the postsynaptic...

  20. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...recombinantly append their proteins of interest with unrelated peptides that already had high quality antibodies...within that region. You’ll notice that “generate a reliable antibody for protein tagging approaches” was not...
Showing: 381 - 400 of 628 results