We narrowed to 631 results for: rel

...cloning efforts. Golden Gate cloning technology relies on Type IIS restriction enzymes, first discovered... destination vector + insert may spontaneously religate, this transient construct retains functional Type...

...attention to the keywords used and think of ways to relate the proposal and deliverables to the aims requested... technologies! If data from a publication or preliminary data suggests the feasibility of the statement...

...socioeconomic or educational background, cultural or religious affiliation, and ethnic or personal identity. ...symposia, or courses in social, health or policy-related topics, as time permits. Also seek out opportunities...

...gene expression and functions of key regeneration-related genes in four inexpensive and commercially available... was first discovered and characterized in an unrelated worm (C. elegans) [2]. Delivering dsRNA matching...

...particularly useful for understanding a specific effect related to an experimental manipulation. Common inbred ...Mice are fed or injected with tamoxifen, which relieves natural inhibition of the Esr1 domain that blocks...

...expand already-existing sets. Note that this tool relies on a stochastic search algorithm rather than pre-designed....8b00333 Pryor, J. M., Potapov, V., Bilotti, K., Pokhrel, N., & Lohman, G. J. S. (2022). Rapid 40 kb Genome...

... choose primary antibodies from more distantly related species and/or use narrowly specific secondary ... antibodies raised in different, and distantly related, species, and secondary antibodies raised in the...

...cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate. Eastman et...human glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library at ...

... from Ashley et al., 2021.   The AID system relies on a transport inhibitor response 1 (TIR1) expressed...induce DSBs within minutes. New CRISPR plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab...

...restriction enzyme digest and compare it to some reliable controls. 2. Viral stocks may be sensitive to ...reducing the culture volume into which the virus is released, a more concentrated virus solution can be obtained...

...more productive and makes it easier to build relationships. Nonetheless, the committees would not function..., I had to learn how to develop new working relationships with these individuals who often had different...

...research goals in focus. Developing these skills relies upon biologists and computer scientists learning...neuron knockouts so that we can perturb factors related to worm motion. Working on the Cat Microbiome Project...

...are much better at remembering these things on a relaxed phone call than when first confronted on a stage... are for presentations, not discussions. I will rarely work with a panel if slides are allowed. Don't ...

...their academic science careers in order to head to Ireland and take a chance as entrepreneurs. To make things...that the learning curve is pretty steep. But we can rely on one another to celebrate good news or share a...

... require careful selection or design to ensure reliable readout and avoid biases. The lab of Alejo Rodriguez-Fraticelli...cell membrane while AF, fused to a payload, is released into the synapse. Once AF binds to the postsynaptic...

...Fusing EGFP to the self-labeling FlavinTag made a reliable one-component magnetoresponsive system that could...Chemical amplification of magnetic field effects relevant to avian magnetoreception. Nature Chemistry, 8...

...serotype, as some rAAV serotypes are abundantly released into the culture media, while others remain cell-associated...Wilson, J. M. (2010). Efficient serotype-dependent release of functional vector into the culture medium during...

...individually count 500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency... anywhere from 10 ng to 1 μg of DNA. It often correlates with the size of the library, but generally needs...

...surrounded by lab members who are all working on related topics, scientific discussion is often part of ...resources on the Addgene blog Check out all careers related blog posts here Find 9 tips for a successful postdoctoral...

...practices for assemblies of more than 10 modules often rely on two-step hierarchical approaches using different...pre-determined manner. If the original insert religates into its original vector, it will subsequently...