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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 718 results for: tat

Showing: 381 - 400 of 718 results

  1. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...inflammation, which can cause mesothelial cells to mutate and ultimately form cancerous tumors.  This process...For example, multiple studies show that certain mutations of the BAP1 gene play a role in susceptibility...individuals, asbestos has not been banned in the United States and many other countries, and it is still imported...

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...at the same time increasing interdisciplinary citations in their own work. Of course not all research ...ImpactStory, a website that helps researchers go beyond citation rates to measure the impact of their contribution...Archambault. "The decline in the concentration of citations, 1900–2007." Journal of the American Society for...

  3. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...maintaining their friendships. I put “neutral” in quotations here because I would not have considered myself...handy: Empathy: If you often find yourself in confrontational situations with others because you simply cannot...competing, or accommodating, if you find yourself gravitating most frequently towards these approaches, you...

  4. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...training and experience, better align trainee expectations with job outcomes, and increase diversity in...need to know in order to even assess the current state of research became more clear throughout the various...information publicly available may realign trainee expectations with actual job outcomes and/or disincentivize...

  5. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...research is dependent on a pathology microtome/cryostat, then making a careful decision on the condition...for suggestions to meet each others’ needs and limitations. Everything is negotiable. Try asking for discounts...research, then it’s worth working within their limitations. 3. Always provide value to others This is a ...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...across the genome) has been implicated in disease states, and targeting the epigenome may make certain processes... spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves methylation kinetics such that cytosine...transduction. Lysine-specific Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9...

  7. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...have intricacies that aren’t always mentioned in static written protocols. It is therefore a bit ridiculous...experiments with too few replicates leading to suspect statistical significance (you can read more about this problem...experimental design practices including talking to statisticians when necessary and encouraging interlab peer...

  8. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...of hands-on lab work for a dissertation. I chose to carry out my dissertation research in a lab in California...outcome for a specific combination of genetic mutations. This is a particular challenge in pancreatic ...

  9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...Kishor Jadhav. Mycoplasmas can remain in the dry state for a longer time. When they come in contact with... is required in this process because result interpretation might be difficult. The low density of mycoplasmas...by these bacteria can hinder correct result interpretation. Also, the signals from the nuclear area make...

  10. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...biochemist, a geneticist, a microscopist, a biostatistician, and that one golden-handed technician who ...Andrew, the team’s founder, to create a mission statement in the first few meetings and we’ve volunteered...like kudzu. Think of a soccer player who gets a reputation for taking risky shots at the goal rather than...

  11. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...Location, location, location! What do buying real estate and using AAVs have in common? The three most important... location, location! Just like this common real estate mantra, the choice of serotype (which determines...identified with AAV2 (5), AAV6 (6) and AAV5 at excitatory connections (3). These facts beg the question...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... most often consist of an enzyme that has been mutated to block its catalytic activity and instead become... later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent binding of the ...work in certain applications — the most common limitation is that certain probes are not very good at crossing...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... to assemble several DNA parts in a specific orientation using Type IIS restriction enzymes (Weber et ... researchers to expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified...acssynbio.3c00423. Pérez-González, Ana, et al. “Adaptation of the GoldenBraid Modular Cloning System and...

  14. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...previously-designed tyr gRNA, which causes hypopigmentation, thus marking cells that have undergone genomic...recombineering, with 96-99% efficiency for point mutations and 66% efficiency for small insertions. Multiplexing...degradation tag (LVA tag) to achieve specific point mutations in E. coli. gRNAs are delivered on a different...

  15. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...Although it was a huge innovation, there was a limitation to this approach. CBEs and ABEs are base transition...repair (BER), the cellular process that catches mutated U’s in DNA and ideally converts them back to Cs... Cas9s that recognize different PAMs. Their computational tool for predicting CGBE editing outcomes and...

  16. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ... had the lowest background activity in the dark state and highest fold induction at 31X. By using a slit...by blue light (470nm), paCas9-1 induced indel mutations via NHEJ (frequency of 20.5%) and induced modifications...significantly alter the system’s efficiency at generating mutations when activated with light and lowered background...

  17. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...sequence cells to verify excision didn’t introduce mutations Adenovirus Non-integrating Low Medium Generates...and/or oncogenes, potentially leading to the presentation of a cancer-like phenotype in infected cells...stringency test for mouse iPSCs, tetraploid complementation, involves injecting tetraploid blastocysts ...

  19. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...Hub in this blog post. Alongside our efforts to facilitate the sharing of reagents and data, we’re also ... first paper came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to ...

  20. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    Published
    May 24, 2016, 2:30 p.m.
    ...Breeding, Genetics, and Biotechnology at Michigan State University with the backing of a prestigious Fulbright... : Kingdom's biotechnology research at Michigan State University 9:36-10:29 : Kingdom's transition back...
Showing: 381 - 400 of 718 results