We narrowed to 460 results for: try

1. Plasmids 101: Gram Negative and Positive Bacteria

   Type

   Blog Post

   Published

   Aug. 2, 2022, 1:15 p.m.

   ... to help visualize the bleached bacteria. The chemistry behind Gram’s crystal violet stain and the ion...

2. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

   Type

   Blog Post

   Published

   June 4, 2019, 1:02 p.m.

   ...variants to eGFP followed by an internal ribosomal entry site and mCherry. If a variant has wild-type levels...

3. Reagent Repositories Are Speeding up Science During the Pandemic

   Type

   Blog Post

   Published

   Aug. 4, 2020, 1:15 p.m.

   ...Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat...

4. Pushing the Limits of DNA Assembly

   Type

   Blog Post

   Published

   Sept. 6, 2017, 1:01 p.m.

   ... led to advances in biofuel generation, green chemistry and understanding the minimal genome. Many of ...

5. Addgene Depositors Get More Citations

   Type

   Blog Post

   Published

   April 20, 2017, 2:30 p.m.

   ...Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology, and Biochemical Research...

6. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   Published

   April 19, 2017, 3:25 p.m.

   ...range of input concentrations (via proprietary chemistry). This turns out to be critically important for...

7. Viral Vectors 101: The AAV Retrograde Serotype

   Type

   Blog Post

   Published

   April 27, 2023, 1:15 p.m.

   ...reagents, AAV particles require receptor-mediated entry into the cell. This means that not all neurons and...

8. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   Published

   May 19, 2020, 1:15 p.m.

   ...Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins. In:...

9. Communicating Your Science With Help From ComSciCon

   Type

   Blog Post

   Published

   July 19, 2018, 1:12 p.m.

   ...works as a data scientist in the entertainment industry.       Additional resources on the Addgene Blog...

10. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...87% mCherry protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR array processing ...

11. Antibodies 101: Monoclonal Antibodies

    Type

    Blog Post

    Published

    June 29, 2021, 1:15 p.m.

    ...Ligand-induced Epitope Masking. Journal of Biological Chemistry 291:20993–21007. https://doi.org/10.1074/jbc.m116.736942...

12. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    Published

    April 5, 2022, 1:20 p.m.

    ...functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method was chosen because ...

13. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    Published

    May 11, 2017, 2:30 p.m.

    ...Pathway Reference Set. These Gateway-compatible entry clones are the product of many years of work and...

14. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...Genetics and Molecular Biology, the Dept. of Biochemistry and Biophysics and the Lineberger Comprehensive...

15. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    Published

    July 10, 2014, 7:17 p.m.

    ...is. While communication styles in academia and industry vary drastically, he reassures that one can learn...

16. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ... multiple copies are required. yes   TRP1 L-tryptophan yes - Grow with 5-FAA. S. cerevisiae no  no TRP1...

17. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ...potluck dinner. Invite guests from a lab, career or industry of interest – learn about their career paths and...

18. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    Published

    May 30, 2023, 1:15 p.m.

    ...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...

19. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...that it is driven by academics, with support of industry partners, not the other way around. Sometimes,...

20. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    Published

    Jan. 14, 2019, 2:08 p.m.

    ...request from us but also by high school students and industry scientists as a universal resource for information...