We narrowed to 842 results for: Eras

...interrogate immune cells to improve immunotherapy in glioblastoma, and generate programmable screening platforms...vectors: The Rubik’s cube of human gene therapy. Molecular Therapy, 30(12), 3515–3541. https://doi.org/10.1016... vector for gene therapy of human diseases. Signal Transduction and Targeted Therapy, 9(1). https://doi.org... methods — in vivo, in vitro, and ex vivo. The general process of these screens is the same: Deliver ...interest (positive or negative selection). Next-generation sequencing (NGS) to identify genes of interest...your library for each experiment. Additional considerations One of the downsides to using AAV is the limited...Cas12a (Cpf1). Even more creative options involve generating strains of mice that stably express Cas effectors...

... Adenovirus Vectors for Gene Therapy, Vaccination and Cancer Gene Therapy. Curr Gene Ther, 13(6), 421–... anecdote goes toward saying that host-virus interactions are an important part of the biology of adenoviruses... the commercial market). Although host-virus interactions likely seem important for any virus, we will...initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies...oncolytic virus in addition to standard uses in gene therapy and in basic research, like other viral vectors...PMID: 31749409. Harrach, B. (2014). Adenoviruses: General Features. In Reference Module in Biomedical Sciences...novo viral gene expression: Implications for gene therapy. Proc Natl Acad Sci U S A, 95(19), 11377. https...

...sequencing (HTS) data we generate and maintain. It became clear that while several people across teams had...enjoy is discussion of current new and exciting literature. As I transitioned out of academia and away from...researchers of all varieties must stay current with literature and emerging technologies to remain competitive...Microbial Pathogenesis that occurred once a week for several months at a time during the spring and fall semesters...club to begin! Figure 1: Discussing next-generation sequencing at the Addgene bioinformatics journal...teams or to stay abreast of current trends in the literature, sampling a breadth of work from a field (perhaps...wandering monologue?  Assuming the feedback is generally positive and the meeting seemed to be of use, ...

...droplet quality. Using a droplet generator, the sample and the droplet generation oil are moved through small...within the expected range. We have also found that generating the standard curve for qPCR can be challenging...Pipette slowly If you are using a manual droplet generator, you will have to be careful about transferring...transferring your droplets from the droplet generator to the PCR plate. Maintaining a high droplet count is important...sample. Even if you are using an automated droplet generator, you should avoid pipetting too quickly when making...rate of 2C/s is recommended to ensure an even temperature change among all of the droplets, but you can...recombinant adeno-associated viral vectors." Human Gene Therapy ja (2019). PubMed PMID: 31140327. PubMed Central...

...disease. This work will hopefully accelerate the development of targeted therapy for peritoneal mesothelioma...learn how plasmids from Addgene will be used to accelerate rare disease research and provide hope to patients...which disruption of the smn1 gene results in the degeneration of spinal cord motor neurons. This disease is...using C. elegans as a model to identify SMN1 interacting partners and perform whole animal screens for...for small molecules that block the neurodegenerative effects of smn1 disruption. As part of his work in...will use Addgene plasmids to characterize smn1 interactions with other genes. He will use fluorescent proteins...of the molecular biology behind SMN1 and its interaction partners, Dr. Di Schiavi will be able to better...

...markers all tagged with mEmerald. Plasmids to monitor protein-protein interactions through multicolor labeling...scale: Look at the mEmerald variants first, followed by the color variants. The mEmerald plasmids offer a...biology, seeing what interacts with what, what affects what. It's like fixing a Maserati. If we know how all...Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many more! Baird has a helpful tip...the Davidson lab always tagged new proteins with mEmerald first, Baird says. From F-Actin to mitochondria... memerald...

...and discovery The first generation of CRISPR could cut DNA, the second generation could do base editing,...specificity: when purified, it could be programmed to generate specific cuts on the RNA target. In addition, ...most common RNA targeting system, Cas13, the "collateral" activity of the enzyme can lead to off targets...RNA editing tools.   Fig. 1 Schematic of luciferase RNA knockdown assays using Cas7-11   Use ...editing, and with the third generation came Prime editing — the ability to now make any small changes, including...small deletions and small insertions, leaving a several kilobase-sized gap in the gene editing field. The...precision into a genome, can be considered the fourth generation of CRISPR, one which drops itself neatly into...

... Choosing a Cas9 for your knockout experiment Generating a no-fuss genetic knockout  Knock-out cells or...loads of sequences to select from - in many cases several exons’ worth of DNA. Any ∼20 nucleotides of DNA...targeting opposite strands must be available in moderate proximity to each other.  If having two sgRNAs...requirements, giving it more flexibility. A consideration with this tool is that the desired edit will...strand break.   Prime editors are the second generation of base modifiers available. This system utilizes...transcriptional activation domain - VP64.  Later generations of activators include the SunTag system which... The Synergistic Activation Mediator complex, generally the strongest activator, consists of a dCas9-VP64...

...and the moderators were on top of any comments that were at all trollish. Kudos to the moderators for keeping...lists of forums on particular topics. Volunteer moderators set the rules for these forums and try to keep...questions! 2. The r/Science subreddit is well moderated. One of our original worries about sharing science...trolls (urban dictionary for trolling: The art of deliberately, cleverly, and secretly pissing people off, ...dialogue). Luckily r/Science has a great team of moderators who facilitated scheduling, filtered out inflammatory... sales-y in posts which falls in line with our general mission to share- as opposed to sell- science. ...expect. Fortunately, Reddit Science is heavily moderated. I started watching the questions and comments...

...practiced or required by many other journals and several funding agencies (e.g. Wellcome Trust, NIH Data...information (or links to information) for assessment of veracity of the conclusions drawn. The main difference ...publication. Who is to say that the initial data-generating lab has efficiently trawled their own data? A...much time they had. It could be argued that data generators may, in light of the policy, hold onto data longer... available to everyone at once, including the generators. This practice doesn’t appear to harm the originating... as in an actual publication and not require interaction with the data authors. In some types of research...multiple animals on different occasions via video, generating huge amounts of data. When published, this data...