We narrowed to 526 results for: SHE

...in opsin dense regions. To overcome this issue, Shemesh et. al. created a soma-targeted derivative of the...the soCoChR-expressing system. Taken together, Shemesh et. al. describe a novel system for the precise...or fluorescent tags are available at Addgene. Shemesh OA, et. al. Nature Neuroscience. 2017. PubMed PMID...

...easy for reviewers to spot when an application is rushed (whether the idea isn’t properly researched / developed...insight into an area - not just working within the established network of your peers. Furthermore, using new...of the work was. Ideally this would be results published in a paper, but positive outcomes can include ...

...Originally published Dec 7, 2017 and updated Jul 2, 2020. Promoters may be the star of gene regulation...Elements) method. The original CAPTURE method was published in 2017 and addressed many of the drawbacks of...where they are physically located. DNA is then sheared into pieces small enough to be pulled down via ...

...Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll SA. Analyzing Copy Number Variation...10.1007/978-1-4939-7778-9_9. PMID: 29717442. Arya M, Shergill IS, Williamson M, Gommersall L, Arya N, Patel ... Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014 Jan 21. doi:10.1002/0471142905.hg0721s80 ...

...techniques. More and more CRISPR tools are being published each month, making CRISPR a great choice for your...to consult the literature to see if anyone has published a protocol that would work for your system. Addgene... are a few plasmids at Addgene that have been published as CRISPR testing tools: Traffic Light Reporter...

...This post was contributed by Max W. Shen from MIT, Alvin Hsu Harvard University, and David R. Liu from...uncertainty. Many thanks to our guest bloggers Max W. Shen from MIT, Alvin Hsu Harvard University, and David... the Broad Institute and Harvard University. Max Shen is a Ph.D. candidate at MIT. His research uses applied...

...removed to leave you with DNA. This DNA is then washed, and either eluted from a fixed surface or precipitated...glass particles are pelleted by centrifugation and washed before DNA is eluted with water or TE. The Bishop...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

...spectrophotometer. Thermo Fisher Scientific Tech Note (2017).  Extinction Coefficients. Thermo Fisher Scientific Tech...Using the NanoDrop One Spectrophotometer. Thermo Fisher Scientific Tech Note (2016).  Loughrey S and Matlock...

...can be eluted once the other materials have been washed away (Figure 1).      Figure 1: The steps...while the media is aspirated. The beads are then washed, followed by antibody elution. Whatever method ... strong ones, consider increasing the number of washes performed during your harvest. Validation Recombinant...

...its various derivatives, biological samples are enmeshed in a gel of a highly absorbent material that is...full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity can...20210110. https://doi.org/10.1098/rsta.2021.0110  Sherry DM, Stiles MA (2022) Improved fluorescent signal...