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We narrowed to 885 results for: ANT;

Showing: 601 - 630 of 885 results

  1. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...including hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades.  So ... cell membrane. Heterotrimeric G proteins are important molecular switches in signal transduction pathways...in peroxidase-catalyzed proximity labeling to quantifiably measure GPCR agonist response in vivo (Paek ...learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic studies ...

  2. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...commonly a plasmid and can infer genetic advantages such as antibiotic resistance. Bacterial conjugation...primary mechanism by which bacteria have gained antibiotic resistance and virulence. Scientists have studied...integration site. The integrated phage remains dormant until it is triggered to enter the lytic cycle....

  3. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...enlarge) As we look ahead to the things to come, we wanted to take a look back at some of the contributions...expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting... from the Trono lab contains the rev gene. It’s meant to be used with pMDLg/pRRE and the envelope expressing...Cas9, this plasmid expresses Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n...

  4. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...have that you really want, please let us know! And if you study chromatin and want to start sharing your...daily basis, the lists below provide a snapshot of relevant resources and a good jumping off point. The following...that histone genes frequently have many different variant names, not all of which can be listed here. If ...

  5. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...and indicate why other neuroscience newbies might want to get into the field. Find Optogenetics Plasmids...There were, of course, many companies selling antibodies, microscopes, and dissection tools, but there...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ...neuroscientist looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene...

  6. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...students and teachers in the U.S. and beyond. “I want to learn more about Synthetic Biology. Can you tell...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff...preparing local (Massachusetts), economically disadvantaged students for paid summer internships. A summer...Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused on education and education...

  7. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Wyatt and Dale Ramsden, UNC at Chapel Hill. One advantage to using the CRISPR/Cas system for genome engineering...process involve repairing chromosomal DSBs. It is important to know that cells possess two major repair pathways... and how these pathways work, as this could be relevant when planning your experiment. Here, we’ll discuss...Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...

  8. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...these people you meet will review your papers or grants in the future, and don’t be surprised to know that...personally Scientists in your field will know about grants and projects that might help your work Tenure can...they have to give you theirs! Think about how you want to introduce yourself and what you do. You can probably...night poker games at Gordon Conferences). You don’t want to be so exhausted that you can’t get a lot from...

  9. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...in my more recent positions outside of the lab. Granted, I currently work on the Scientific Support Team...thesis as it is for helping your team reach an important milestone at work.   While I was in graduate school...work through it. Ask yourself if you missed any important details. Or challenge yourself to think of more...that this skillset is a part of your toolkit is important. Paired with your other skills, both technical...

  10. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...newest CRISPR plasmids available at Addgene. If you want to read more content related to CRISPR technology...making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be effective... the cellular MMR machinery is disrupted via a dominant negative mutation in MLH1 (PE4 = PE2+MLH1dn and...improve gene editing performance at therapeutically relevant gene targets and expand the application to the...

  11. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...doing interesting and relevant work in your field! However, there’s no guarantee you’ll get a response...to make plasmids that already exist, and we significantly reduce costs of producing plasmids with high-throughput...always faster to order from us!) Ask the PI If you want a plasmid for free, you can always reach out to ...

  12. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...biomedical workforce. Quotes taken directly from participants (summarized in 2) touched upon many problems...better solution would be to reduce the number of entrants into PhD programs to more closely reflect the ...Labor Market. On this topic it is clear that many important questions, areas for informed discussion, debate...Among other involvements, he helped to establish grants through the Sloan foundation for postdoc-related...

  13. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...cells are incubated and after several days the supernatant is collected, filtered, and titered. A major ...number of harvests will all impact the titer. Importantly, optimized conditions for one transfer plasmid...generally a good place to start. If you don’t anticipate that you will need large volumes of lentivirus...producing lentivirus, it is time to titer. One important consideration when titering a lentivirus is the...

  14. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...undergraduate students (1, 2), but educators face significant challenges in designing suitable projects. In...because the S. cerevisiae deletion strains are ∆ura3 mutants and cannot grow without uracil supplementation ... Students who participate in the course show significant gains in both content knowledge and research ...PMCID: PMC4012490. 4. Hoffman, C.S., Wood, V., & Fantes, P.A. An ancient yeast for young geneticists: A...

  15. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...subsequent conclusions. Being vigilant about these errors is an important part of maintaining the quality...elements, and nucleotide indels or mismatches in significant plasmid elements. Which differences are considered...This post was written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley...

  16. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...decision easier. 1. Müller, Sara M., et al. "Quantification of Förster resonance energy transfer by monitoring...monitoring sensitized emission in living plant cells." (2013). PubMed PMID: 24194740. PubMed Central PMCID...) or would perturb the endogenous state that you want to measure (overexpressing a gene or protein after...detect cellular processes and signaling cascades, quantify mechanical tension (a molecular “spring”) (Meng...

  17. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...could have wanted to know was just buried beneath someone's lunch. Many times, I’m pleasantly surprised...should not) answer your questions about an Abcam antibody. Have your catalog number ready. I've attempted...last digit: An answer should come soon, but not instantly. Addgene tries to get back to customers within...

  18. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ... transposon methylation underlies the mantled somaclonal variant of oil palm." Nature 525.7570 (2015):...study,epigenetic changes were statistically significantly associated with reduced life-span, but no changes...controlled, the reporter consistently showed significantly different FRET levels between unmethylated and...

  19. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...management skills but a longer term and more substantial experience can be achieved by volunteering for...national issues. I applied and was successfully granted a travel award to attend the annual NPA meeting...decide to remain here.  In a time when it is so important for postdocs and graduate students to think about...blogger Juliet Moncaster! Juliet Moncaster is an Assistant Professor at the Boston University School of Medicine...

  20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...expressed, they predominantly fluoresce one color but slowly mature until they predominantly fluoresce a ...FP timers can be used in any situation where one wants to understand the relationship between the age of...3. Verkhusha, Vladislav V., et al. "An enhanced mutant of red fluorescent protein DsRed for double labeling...

  21. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...repairing DNA double strand breaks – (HR). HR is the dominant homology-directed repair (HDR) pathway in mammalian...higher HR frequencies than quiescent cells. It is important to know that HR is often mutated in cancer cells...recognition sequence in the donor DNA, you can guarantee there won’t be re-targeting after HDR. If your...your cells. Enrich S-phase cells HR operates predominantly in S and G2 phase of the cell cycle, when a ...

  22. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ... popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...transduced with a lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013...contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer vectors...

  23. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...activator (1a) or a repressor (1b). Scientists took advantage of these discoveries to create a new system for...mating two single haploid yeast fusion plasmid transformants into a diploid strain for screening), or arrayed...increase the stringency of your binding. And most importantly: Independently validate identified binding partners...ensure that your setup works. The last thing you want is to run your lovely library screen only to find...

  24. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...the thousands of other scientists using pLKO.1 variants learn about those updates? This is a problem many...resources can be found in our Plasmids 101 blog series. Want to learn more about sharing your plasmids via Addgene...looking to streamline your lab management and you want the works: organizing reagent storage and inventory... plasmid features, and reference tables (i.e. antibiotic concentrations, commonly used primers). We also...

  25. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...into AAV. NmeCas9 has the added advantage that it can be turned off by anti-CRISPR proteins. The Sontheimer...genome modification, but they did so without a substantial immune response or toxicity. More AAV-based CRISPR...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

  26. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...comparable (within r2=0.96) to that of NGS data. Advantages of ICE Determine Editing Efficiency and Types...  ICE uses Sanger sequencing data to produce quantitative, NGS-quality analysis of CRISPR editing - enabling...guide design tool, guide validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please...featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility RNA Editing with Cas13 and...

  27. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ... allows access to a larger number of willing participants. While fibroblasts obtained from aged patients...hiPSCs than age-matched fibroblasts [3]. These advantages make PBMCs the ideal source of somatic cells ... vectors are based on the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA-1) system where each vector contains...OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method had a reprogramming efficiency...

  28. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ... as pre-made viral preps. If you can’t (or don’t want to) make viral preps in your lab, there are custom...Titering After generating your lentivirus, you’ll want to infect your cells with the virus–the right amount...conditions to optimize include incubation time, antibiotic conditions, and amount of polybrene. (psst…didn...have a large number of cells to transduce, you may want to consider spinfection. Spinfection is the process...

  29. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...that asbestos exposure remains the single-most important factor in developing mesothelioma. Asbestos, and...cells that can begin an immune response. This important biomarker can be used to distinguish between three...earlier, thus improving the patient’s prognosis significantly, but also to monitor those who may be at greater...be Used to Study Cancer Drug Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional...

  30. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...These differences in recombinase efficiency are important to keep in mind when deciding whether to use the...common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain (LDB...recombinase. The high concentration of FLEx vectors guarantees that most cells are transfected with all labeling...versions can be used in these experiments. This elegant combination of Cre, FLP, and inducible systems ...
Showing: 601 - 630 of 885 results