We narrowed to 842 results for: Eras

...and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly There are a few important points... when choosing the vector you’d like to use to generate your new fly line: 1. Where would you like the...  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut. Cell...

... the “Analyze Sequence” button to pull up an interactive plasmid map powered by SnapGene and gain access...the static maps discussed above, but also have interactive functionalities. You can now hover over any feature...maps, the linear sequences hosted here are now interactive and have similar hover over features. As with...

...closely associated and function as one unit: Gβγ. In general, GPCRs show a propensity to associate with certain...receptor, which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed ...Chemogenetics uses genetically engineered receptors to interact with small molecules to elicit a response in a...

... the trace; asterisk indicates a single AP. B) Averaged fluorescence responses to increasing numbers of...International license. Using OCaMP in your lab Several OCaMP constructs are now available through Addgene.../article/28248224/","type":"EXTERNAL"},"user_generated_content":false}{% endraw %}{% end_module_attribute...

...in a cell or animal model. AAV plasmids contain several important elements, but among the most important...recombination. How do I validate my ITR sequences?  Next-generation sequencing and Sanger sequencing might fail to...vector stability and sequencing results. Next-generation sequencing is also helpful in quantifying ITR...

...protein is based on the properties found in the literature or tabulated data that summarizes properties ...). However, the theoretical brightness ignores several important experimental conditions that are related...detector sensitivity) and the biological system (temperature, prokaryote versus eukaryote, background fluorescence...

...Plasmids 101 will provide you with an overview of general molecular biology knowledge and techniques. If ...with only white colonies is very suspicious! Refrigerate your plates: Placing plates at 4C for few hours...care in making your plates: X-gal is light and temperature sensitive and needs to be added to media after...

...exposed to 500 nm violet light, the Dronpa dimers interact and lock MEK1 in an “off” setting by blocking ...possible to create other photoswitchable kinases? The general design of psMEK1 also worked for creating three...all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids...

...from peer-reviewed publishing in a journal.  Accelerating scientific communication Preprints have been...Figure 1: Stand aside, CERN — it's a new age of accelerating science! Photo courtesy of Henry Mühlpfordt,...attendees to read and comment online! Want the general public (say, policymakers or community organizations...

...CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation that will permanently ablate...package cDNAs for genomic delivery and have many therapeutic advantages, such as tissue-specific targeting... to temporarily increase expression. There are several flavors of activators which can be used with this...

...the photostability measurements reported in the literature are performed in different ways. We describe ...intensity remains. The first issue is that, in several cases, the fluorescence intensity decay of a fluorophore... be identified by a head-to-head comparison of several fluorescent proteins of the same spectral class...

...implementing consensus recommendations, even when a federal mandate encourages them to do so. Immediately after...There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary and Beth Israel...salaries when all others in Boston (some of them literally across the street from these institutions) are...

... of this is gospel, but we think it's worth consideration. Everything will go wrong Whenever an order ... lab, a similar situation could arise if a lab veteran who worked with a particular sample suddenly left...easily as needed. In this case, the physical considerations of the laboratory make object composition a...

... randomly. Therefore, inserting loxP sites at deliberate locations in a DNA sequence allows for very specific...reciprocal crossover event. This event can have three general outcomes based on the location and orientation ...separate DNA molecules, a translocation event is generated at the loxP sites. How can I use Cre-lox? The ...

...and think of ways to relate the proposal and deliverables to the aims requested in the call. Step 2: ...technologies. Therefore, take time to review the literature and offer educated insight into what the desirable...each aim. A funded proposal typically lists the deliverables, explains 1-2 potential pitfalls of the intended...

... the right choice. I spoke with our Financial Operations Specialist, Lucy Hammer, about why she did not...question came from Lucy Hammer, our Financial Operations Specialist. She said, “I’ve found the most purpose...the office. Main takeaways from Meet an Addgenie Overall, the Meet an Addgenie project was one of the biggest...

... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...populations. All of these topics require additional considerations to resolve. Sequencing and assembly issues ...acquired from the MiSeq for assembly or with enough coverage over plasmid regions to confirm their presence...

...select the components they want for each module, generate the vector file, and quickly clone their vector... Step 4: Naming your assembled plasmid and generating the vector file on the Fragmid website. ...4, is to give the assembled plasmid a name and generate the vector file. Ta-da! Most (180/181) Fragmid...

...format that was stable for up to a year at room temperature (Pardee et al, 2014). As a demonstration of the...the project by initially designing and ordering several dozen versions of the toehold switches and amplification...Addgene As we report, we have embedded this next generation of toehold switch-based RNA sensors within an...

...in AT-rich genomes. Additionally, whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1...with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and 19-23...sticky end” transgene insertion mediated by Cpf1-generated overhangs. Exploring the efficiency of these two...

...and mutant loxP sites has allowed scientists to leverage this system in new, creative ways. Today’s post...potential to express the transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic...Double-floxed inverse Orientation), FLEx systems are generally considered "Cre-On" meaning that your gene of ...

...drawn to the idea of working as a team to develop therapies that could directly impact patients. And, to be...beginning of March, and the jobs are mixed into the general careers section of their web site. Each position... and is applying this towards researching neurodegenerative diseases.   Additional resources on the Addgene...

... modulators, and chimeric effectors. Nucleases generate a classic double-stranded break, while modulators...with researchers all over the world. That’s an average of 27,272 plasmids distributed per year, or approximately...it clear that it’s also a story of community, cooperation, and sharing. Sharing this story is a truly special...

...specific recombination outcome using PCR and then generate a monoclonal cell line with the floxed exon. You...B, A Limaye, & AB Kulkarni. (2009) "Overview: Generation of Gene Knockout Mice". Curr Protoc Cell Biol...Basics of Homology Directed Repair Read about Generating Knockouts with CRISPR through Non-Homologous ...

...quick tips to get things going: If your PTA has a general meeting and a means to request funding for events...and think of a way to make this section more interactive. If you can think of a good analogy that involves...but I also tried not to be an alarmist.  The overarching guideline for all of these questions, and for...

...Figure 1B-D). These other molecules can alter the overall 260 nm reading, distorting the final concentration...with dilute samples:  Take multiple readings to average out noisy measurements. Be cautious when interpreting...these long enough?  There are, of course, more considerations and other approaches for measuring DNA quantity...

...deprioritize genes that could be good targets for therapeutic development, particularly in cancers that are... ancestry which will reduce the likelihood of generating genetic dependency data with an ancestry-based...researching ancestry groups. And, of course, it’s imperative for scientists to be aware of and actively use...

...connection levels and interaction. Can bring in things to be physically interacted with. Public speaking...s much more challenging to design a hands-on interactive activity using elephants than it is to design...

...needed for EM is not something you find in the average cell and molecular biology lab, and EM is limited... is able to expand evenly in all directions to several times its original size. Depending on the protocol...depend upon the specific gel recipe used, ambient temperatures, incubation times, and the physical properties...

... Prevotella and Francisella), was annotated in several genomes and is classified as a type V CRISPR system...Again, they were in for a surprise! Whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1...with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and 19-23...