We narrowed to 827 results for: nin

...subject to mishandling?  Is the experiment you’re planning to run with the antibodies time-sensitive, using...which you may need in your experiment.  If you are planning to conjugate your antibody in the lab, you’ll ...

...you are in the design phase of your experimental planning, you need more nitty-gritty details — how can ...link to search Addgene's collection for plasmids containing that enzyme, and embedded in the References lists...

...with powerful cognitive biases such as motivated reasoning and hindsight bias, we may fool ourselves and ...discoveries Finally, there are good reasons for obtaining a different result after conducting a replication...

...Developing science communication skills, while also learning about new scientific developments and helping ...s advice to tap into the online conversations happening around a scientific conference, whether you are...

...chlorophenol red-b-D-galactopyranoside on the assay paper turning it purple, allowing for easy visual detection of...Sidhu’s group has made several hundred SH3 domain containing plasmids available through Addgene. These plasmids...

...bootstrap your science communication skills with training programs, which are increasingly available. But... draw and the perspective I want them to form? Training in science communication should prepare you to...

...tools, where light used for excitation can cause unintended activation of the optogenetic system. Biosensors... cellular physiology in your system. Optical sectioning cannot be conducted with luminescence imaging...

...pre-crRNA arrays simplifies the crRNA cloning process. For cloning, Zetsche et al used four oligos that...

...Institute have supported the AAV initiative from the beginning and have worked closely with us to functionally... isn’t the only facet of a project this big. Maintaining our excellent service and accuracy involves caring...

...command line, and so was really starting from the beginning. Luckily, a fellow graduate student and a data... entered is useful for beginners who are still learning the basics. However, these notes take up a lot...

... for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). If the reviewers...future. Disregard reviewer’s comments without explaining why. If it looks like you have ignored their ...

... of solute channels that are responsible for maintaining the nutrient and ionic balance of the cells,”...the skin making it important to study what is happening within the keratinocytes. “We hope to use CRISPR...

...Do I have to be on LinkedIn?” For anyone who is planning or might need to plan for a career outside academia...hiring managers will think it is odd. For those planning a career in academia, it may not be required, ...

... used in research have their genomes “gutted”, meaning that all viral genes except ITRs (inverted terminal...capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your gene of interest (or genes) need to be...

...target opposite DNA strands and specify a large intervening deletion. Not only can this method precisely ...induces two double-strand breaks and deletes the intervening sequence. As with other prime editing tools, ...

...membrane. The haemagglutinin (HA) tag is a small 9 amino acid epitope of the haemagglutinin of the influenza...

...of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters To keep gene expression stable...components. When your pathway needs precise fine-tuning of gene expression levels, give these stabilized...

...interactions, Ramanathan et al. designed an RNA bait containing three well conserved but not well characterized... and post-transcriptional regulation of SM1v1-containing RNAs could play a role in the severity of breast...

... additionally provides detailed information on cloning procedures used to construct the deposited plasmids...Considerations when getting the dTAG system up and running in your lab Confirm the functionality of your fusion...

... two dimerizing photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation) domains... psMEK2, psRaf1, and psCDK5. MEK2 is a serine/threonine kinase with a similar 3D structure to MEK1, but...

...coregulator-related global gene profiling datasets in to our data mining tool, Transcriptomine. We are currently nearing...2001). Nuclear receptors and lipid physiology: opening the X-files. Science 294, 1866-1870. (PubMed) Lonard...

... about a week. Taking advantage of Golden Gate cloning, the Haynes lab’s Protocols.io protocol describes...useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged CRISPR knock-in...

...anti-CRISPRs for a type II system in Neisseria meningitidis (Nme) (Pawluk et al., 2016). While Streptococcus...most used and well studied CRISPR system, N. meningitidis can also be used for genome editing. In this...

...ncRNA locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for targeting thousands...092902 Liu Y, Cao Z, Wang Y, et al. Genome-wide screening for functional long noncoding RNAs in human cells...

...with more stringent stripping methods. If you are planning to detect multiple proteins through several rounds...membrane once before any detection steps to avoid retaining residual antibodies. If you have already applied...

...differently (distracted) from the ones tested at the beginning. Variability source Standardization parameters...coordination to more complex situations such as learning and memory or emotional states, for example anxiety...

...Several variants are now circulating and plasmids containing these mutations have been deposited at Addgene...more about it on the blog. COVID-19 research is happening fast and we’re glad to join these efforts and ...

...architecture and plumbing are important. We started with learning the head is connected to the neck. Shortly after...M, Ghanem A, Conzelmann KK, Lepier A, Götz M, Berninger B. Retrograde monosynaptic tracing reveals the...

...spread out the risk for the project by initially designing and ordering several dozen versions of the toehold...genome as unique barcodes to identify samples containing the virus. Of the 48 sensors, targeting 24 regions...

...the post-transcriptional additional of multiple adenine (A) nucleotides to the tail of a messenger RNA ...Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids 101 Posts ...