Addgene

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Showing: 401 - 420 of 441 results

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Harnessing Bacterial Toxins for Allelic Exchange

Type

Blog Post

Published

Aug. 15, 2019, 12:30 p.m.

...the efficiency of this versatile technique. Many thanks to our guest blogger Jacob Lazarus from Harvard...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

Published

April 9, 2019, 1:13 p.m.

...potential negative consequences of the tag. Many thanks to our guest blogger, Joachim Goedhart! Joachim...
The Fluorescent Vegetables in Aptamer Soup

Type

Blog Post

Published

Jan. 29, 2019, 2:27 p.m.

...PubMed Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification...
Antibodies 101: Introduction to Immunofluorescence

Type

Blog Post

Published

Nov. 16, 2021, 2:15 p.m.

...all claim to target your protein of interest. Thankfully, there are a number of websites compiling antibody...
Quick Guide to Near-Infrared Fluorescent Proteins

Type

Blog Post

Published

Oct. 5, 2021, 1:15 p.m.

...choosing the perfect one for your experiment! Thank you to our guest blogger! Daria Shcherbakova is ...
Filming Science Videos in the Age of Social Distancing

Type

Blog Post

Published

May 20, 2021, 1:15 p.m.

... but we needed someone else. Design: Jennifer thankfully volunteered, and we decided to focus on a protocol...
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

Type

Blog Post

Published

May 5, 2020, 1:15 p.m.

...Addgene's COVID-19 Plasmids and Resources Many thanks to our guest bloggers Shravanti Surest from Iowa...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Type

Blog Post

Published

Sept. 5, 2017, 1:58 p.m.

...Cas-blocking mutations into the HDR repair template. Many thanks to our guest bloggers Dominik Paquet and Dylan ...
Fluorescent Proteins 101: When GFP lets you down

Type

Blog Post

Published

Aug. 23, 2018, 12:05 p.m.

...effort of numerous scientists world-wide. Many thanks to our guest blogger, Joachim Goedhart! Joachim...
Replacing Paper: Tips for Choosing an Electronic lab Notebook

Type

Blog Post

Published

July 10, 2018, 1:08 p.m.

...the lab a serious competitive advantage. Many thanks to our guest blogger, Tea Pavlek, Product Marketing...
Multiple Plasmids at a Low Price: Kits from Addgene

Type

Blog Post

Published

Sept. 14, 2016, 2:30 p.m.

...peptides including antibiotic efflux pumps and ankyrin seven-repeat proteins: Sjuts, Hanno, et al. "Molecular...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

Published

June 23, 2016, 2:30 p.m.

...phosphoproteins’ available on the Addgene website. Many thanks to guest blogger Natalie Niemi! Natalie Niemi, ...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

Published

March 1, 2016, 3:30 p.m.

...catalyze directional cloning of DNA parts that are flanked by orthogonal versions of the attB and attP sites...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

Published

Jan. 16, 2025, 3:42 p.m.

...submit your validation data after your experiment! Thanks to Addgenies Brian O’Neill and Ina Ersing for contributing...
How to Design Your gRNA for CRISPR Genome Editing

Type

Blog Post

Published

Sept. 24, 2020, 1:15 p.m.

...experimental design, execution, and analysis. Many thanks to our guest blogger John Doench! John Doench is...
Plasmids 101: Dimers and Multimers

Type

Blog Post

Published

Jan. 10, 2023, 2:15 p.m.

...kb). Image generated by SnapGene software. Thanks to increasing affordability and accessibility, ...
Antibodies 101: The Basics of Western Blotting

Type

Blog Post

Published

March 8, 2021, 2:41 p.m.

...system the sandwich is completely submerged in a tank filled with a transfer buffer formulated to conduct...
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Type

Blog Post

Published

April 5, 2018, 12:42 p.m.

...high-concentration, column-purified DNA template. Many thanks to Channabasavaiah B. Gurumurthy, Masato Ohtsuka...
Technologies Enabled by NanoLuc® Luciferase

Type

Blog Post

Published

Feb. 8, 2018, 12:17 p.m.

...to see how you utilize them in your group! Many thanks to our guest blogger Kyle Hooper. A former Technical...
Generating Mouse Models Using CRISPR/Cas9

Type

Blog Post

Published

July 12, 2016, 2:30 p.m.

...much to learn from biology in all its forms! Many thanks to our guest bloggers, Wenning Qin and Haoyi Wang...

Showing: 401 - 420 of 441 results

Results per page:

Harnessing Bacterial Toxins for Allelic Exchange

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

The Fluorescent Vegetables in Aptamer Soup

Antibodies 101: Introduction to Immunofluorescence

Quick Guide to Near-Infrared Fluorescent Proteins

Filming Science Videos in the Age of Social Distancing

SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Fluorescent Proteins 101: When GFP lets you down

Replacing Paper: Tips for Choosing an Electronic lab Notebook

Multiple Plasmids at a Low Price: Kits from Addgene

Using Phosphoserine to Study Protein Phosphorylation

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Viral Vectors 101: AAV Serotypes and Tissue Tropism

How to Design Your gRNA for CRISPR Genome Editing

Plasmids 101: Dimers and Multimers

Antibodies 101: The Basics of Western Blotting

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Technologies Enabled by NanoLuc® Luciferase

Generating Mouse Models Using CRISPR/Cas9

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