We narrowed to 557 results for: ESP
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TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m....abdomen to produce yellow-green light (Figure 2). Despite the title of this post, this reaction is not fluorescence...
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Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....normally inactive and cannot bind the tetracycline response elements (TRE) in a promoter. Tetracycline and... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....together encode the N and C terminal segments respectively of a GFP dependent Cre. The two segments come... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog PostPublishedJan. 30, 2020, 2:15 p.m....they actually are. Throwing out plates that don’t respond as expected, use of representative images, measuring... -
Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m....provide a better understanding of the key residues responsible for this protein-drug interaction. The Dueber... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog PostPublishedFeb. 19, 2019, 1:36 p.m.... he leads the Internal Audit function and is responsible for bringing a systematic and disciplined approach... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....target specific mRNA transcripts for degradation. Despite how easy these experiments are to physically set... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....services. There was an overwhelmingly positive response to the option of Addgene providing plasmids in... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....practices suggested here may seem tedious at first, especially since you’ll want to dig in to the computation... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....plasmids up to 25 kb, most cloners should be fine, especially using plasmids in the 10-12 kb range. REPLACR-mutagenesis... -
Plasmids 101: Blue-white Screening
TypeBlog PostPublishedJune 4, 2015, 1:03 p.m....Although blue-white screening is probably the most widespread way to select for plasmids containing an insert... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....of PTG1 and PTG2 were 3 and 31 times higher, respectively. In rice protoplasts, Xie et. al observed slightly... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m.... of the technology to the public in an open, responsible manner. At Revolution Bio, we are addressing ... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m....kit also has other unique aspects that make it especially well-suited for use in stem cells. (See the complete... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....gut can provide insight into how the microbiome responds to infection, dysbiosis, and antibiotic treatment... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....negative regulation by using a promoter that is responsive to a repressor protein, the increase in expression... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....is characterized by point mutations in the Iron Response Element (IRE) RNA motif of the ferritin light ... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....recent papers noted below for examples of dose-responses and time-courses (Nabet et al., Erb et al., Huang... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....cover all of the possible antibody conjugates (especially if you start to consider clinical applications... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....phosphorylated MEK1/2 levels, suggesting PP1/PP2A is responsible for this MEK1/2 negative feedback. 3. Studying...