We narrowed to 553 results for: PPL

1. Improving Transduction Efficiency with Exosome AAVs

   Type

   Blog Post

   Published

   March 14, 2019, 1:54 p.m.

   ... This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV antibodies...

2. Pushing the Limits of DNA Assembly

   Type

   Blog Post

   Published

   Sept. 6, 2017, 1:01 p.m.

   ...transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...

3. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   Published

   July 21, 2017, 12:48 p.m.

   ...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...

4. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   Published

   April 19, 2017, 3:25 p.m.

   ... we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation Technology for...

5. Reagent Repositories Are Speeding up Science During the Pandemic

   Type

   Blog Post

   Published

   Aug. 4, 2020, 1:15 p.m.

   ... Another example of reagents that have broad applications but have proven useful to the study of SARS-...

6. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

   Type

   Blog Post

   Published

   July 21, 2020, 1:15 p.m.

   ...you choose could be based on your downstream application, time, and instrument availability. UV absorbance...

7. Multiplexed Overexpression of Regulatory Factors (MORF) collection

   Type

   Blog Post

   Published

   March 30, 2023, 1:15 p.m.

   ...single TF was delivered to each cell, future applications could deliver multiple TFs to each cell, allowing...

8. Scientists Map the SARS-CoV-2-Human Interaction Network

   Type

   Blog Post

   Published

   April 9, 2020, 1:15 p.m.

   ...requests (175 and counting) that we ran out of FedEx supplies at one point and had to go begging for them from...

9. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

   Type

   Blog Post

   Published

   Nov. 27, 2018, 2 p.m.

   ...collaborations. Many thanks to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and...

10. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type

    Blog Post

    Published

    Nov. 20, 2018, 8:17 p.m.

    ...number and reagent description is lost (e.g. a supplier is bought out by another company and catalog number...

11. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...incorporate FPbase data in their own projects and applications, a REST API allows programmatic retrieval of...

12. 5 Great Apps for Lab Life

    Type

    Blog Post

    Published

    Sept. 22, 2016, 2:30 p.m.

    ...lab notebook. Lab Archives is a multipurpose application that allows you to share your data with colleagues...

13. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    Published

    March 24, 2016, 2:30 p.m.

    ...altering its function). HDR is crucial for certain applications, for example, correcting the allele that causes...

14. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type

    Blog Post

    Published

    Aug. 28, 2014, 3:34 p.m.

    ...High Fidelity versions of the endonucleases and/or supply custom buffers as means to avoid this issue. ...

15. Finding and Joining Your Dream Lab

    Type

    Blog Post

    Published

    April 8, 2014, 2:50 p.m.

    ...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...

16. Plasmids 101: Mammalian Vectors

    Type

    Blog Post

    Published

    March 25, 2014, 3:15 p.m.

    ...your cells divide. Gentamicin is often used as a supplement in mammalian cell culture to suppress bacterial...

17. Fluorescent Proteins 101: Luciferases

    Type

    Blog Post

    Published

    July 22, 2025, 1:15 p.m.

    ...can also allow multiplexing with experiments that supply both substrates and measure luciferase output at...

18. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...

19. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type

    Blog Post

    Published

    June 25, 2019, 12:54 p.m.

    ...Scientists can also use electroporation, the application of an electrical charge to cells, to increase...

20. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...